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K2edta vacutainer blood collection tubes

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K2EDTA vacutainer blood collection tubes are used for the collection, transportation, and storage of venous blood samples. The tubes contain the anticoagulant K2EDTA, which prevents the blood from clotting during the collection and handling process.

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Lab products found in correlation

Erythrocyte depletion kit, by Miltenyi Biotec (1 mentions) Macsxpress whole blood neutrophil isolation kit, by Miltenyi Biotec (1 mentions)

Close spelling variants of this product

BD Vacutainer (1960 citations) Vacutainer tubes (1123 citations) K2EDTA (197 citations) K2EDTA vacutainers (70 citations) K2EDTA tubes (66 citations) Blood collection tubes (47 citations) K2EDTA collection tubes (2 citations) K2EDTA blood tubes (2 citations)

5 protocols using k2edta vacutainer blood collection tubes

1

Isolation and Characterization of PBMCs from Whole Blood

Cited 2 times
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As previously published20 (link),21 (link),69 ,123 , whole blood was collected in K2EDTA vacutainer blood collection tubes (BD, 366643) and kept at room temperature for up to 2 h prior to PBMC isolation. Briefly, blood from healthy volunteers and PD patients was overlaid in Leucosep tubes (Table 2) for PBMC isolation, centrifuged for 20 min at 400 g with brakes turned off and acceleration set to minimum. PBMCs were collected from the interphase of Ficoll and PBS, transferred to a fresh 15 mL conical tube, resuspended in 8 mL sterile PBS and centrifuged for 10 min at 100 × g, and repeated twice more. Cells were counted with a hemacytometer using trypan blue exclusion of dead cells, and density adjusted with PBS for flow cytometry staining.
Gopinath A., Mackie P., Hashimi B., Buchanan A.M., Smith A.R., Bouchard R., Shaw G., Badov M., Saadatpour L., Gittis A., Ramirez-Zamora A., Okun M.S., Streit W.J., Hashemi P, & Khoshbouei H. (2022). DAT and TH expression marks human Parkinson’s disease in peripheral immune cells. NPJ Parkinson's Disease, 8, 72.
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2

Isolation and Characterization of PBMCs

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Whole blood was collected in K2EDTA vacutainer blood collection tubes (BD, cat. 366643) and held at room temperature for up to 2 hours prior to PBMC isolation. Primary antibodies were used as listed in Table 2. In brief, primary antibodies MAB369 and AB152 (Table 2) were used after fixation and permeabilization to detect DAT and TH respectively. Fluorochrome-conjugated anti-CD14 (MP19, BD) was used to detect CD14. Whole blood from healthy volunteers was overlaid in Leucosep tubes (Table 3) for PBMC isolation; standard 15mL polypropylene conical tubes were used for isolation from enriched leucocytes. Specific centrifuges used are listed in Table 1.
Gopinath A., Doty A., Mackie P.M., Hashimi B., Francis M., Saadatpour L., Saha K., Shaw G., Ramirez-Zamora A., Okun M.S., Streit W.J, & Khoshbouei H. (2019). A novel approach to study markers of dopamine signaling in peripheral immune cells. Journal of immunological methods, 476, 112686.
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3

Isolation of Primary Human Neutrophils

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Primary human blood samples were obtained with informed written consent from healthy human adult donors in accordance with Pfizer Inc. Global Occupational Health and Wellness Research Support Program (protocol GOHW RDP-01) approved by the Schulman Institutional Review Board. Peripheral blood was obtained by venipuncture and drawn into K2-EDTA vacutainer blood collection tubes (BD Biosciences, Franklin Lakes, NJ) prior to experimentation. Neutrophils were isolated using the MACSxpress Whole Blood Neutrophil Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) following the manufacturer’s instructions. For neutrophil purification, red blood cells were depleted using the Erythrocyte Depletion Kit (Miltenyi Biotec) following the manufacturer’s instructions. Isolated neutrophils were washed 3x with the respective assay buffer to completely remove serum and EDTA.
Su Z., Brooks J., Pelker J., Andreyeva T., Sobon H., Gifford R., Powers M., Wang J., Dower C., Hegen M., Messing D., Sheehan A.D, & Brennan J.J. (2023). Studies with neutralizing antibodies suggest CXCL8-mediated neutrophil activation is independent of C-C motif chemokine receptor-like 2 (CCRL2) ligand binding function. PLOS ONE, 18(1), e0280590.
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4

Bovine Genotyping and Phenotyping Protocol

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DNA was extracted from blood samples which were collected in 9 ml K2EDTA Vacutainer blood collection tubes (BD Diagnostics) by jugular venepuncture. These samples were inverted to mix and prevent clotting, immediately placed in isothermic boxes, and transferred to the laboratory. During sampling animals were handled by qualified professionals who observed the procedure of well-being and safety of all animals.
A high-density SNP panel (BovineHD BeadChip assay 700k, Illumina Inc., San Diego, CA) was used for genotyping. SNP markers with minor allele frequency (MAF) and call rate less than 2% and 98%, respectively, were excluded. Also, samples with a call rate less than 90% were not considered in analyses. After genomic data quality control, 437,197 SNPs from 761 animals were available, of which 721 had phenotypes for LMA, 669 for BF, and 718 for RF trait (Table 1).
Medeiros de Oliveira Silva R., Bonvino Stafuzza N., de Oliveira Fragomeni B., Miguel Ferreira de Camargo G., Matos Ceacero T., Noely dos Santos Gonçalves Cyrillo J., Baldi F., Augusti Boligon A., Zerlotti Mercadante M.E., Lino Lourenco D., Misztal I, & Galvão de Albuquerque L. (2017). Genome-Wide Association Study for Carcass Traits in an Experimental Nelore Cattle Population. PLoS ONE, 12(1), e0169860.
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5

PBMC Isolation from Whole Blood

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PBMCs express TH 43, (link)51 (link) . As previously published 51 (link) , whole blood was collected in K2EDTA vacutainer blood collection tubes (BD, 366643) and held at room temperature for up to 2 hours prior to PBMC isolation. Briefly, blood from healthy volunteers and PD patients was overlaid in Leucosep tubes (Table 2) for PBMC isolation, centrifuged for 20 minutes at 400g with brakes turned off and acceleration set to minimum. PBMCs were collected from the interphase of Ficoll and PBS, transferred to a fresh 15mL conical tube, resuspended in 8mL sterile PBS and centrifuged for 10 minutes at 100g, and repeated twice more. Cells were counted with a hemacytometer using trypan blue exclusion of dead cells, and density-adjusted for downstream applications.
Gopinath A., Badova M., Francisa M., Shaw G., Collins A., Miller D.R., Hansen C.A., Mackie P., Tansey M.G., Dagra A., Madorsky I., Ramírez-Zamora A., Okun M.S., Streit W.J., & Khoshbouei H. (2021). TNFα increases Tyrosine Hydroxylase expression in human monocytes.
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