400 mesh formvar and carbon copper grids

Transmission electron microscopy (TEM) was performed using 400-mesh formvar and carbon copper grids (ProSciTech, Townsville, Australia) as described previously [28 (link)]. Bacteriophage stocks (>10⁷ PFU/mL) were allowed to adsorb to the grid for one min before excess solution was removed using filter paper. The grids were then negatively stained three times with 2% (w/v) uranyl acetate for 20 s. Excess stain was removed by filter paper and grids were air-dried for 20 min before examination under a JEOL JEM-2100 transmission electron microscope. This was operated at an accelerating voltage of 200 kV and high-resolution digital images were recorded on a Gatan Orius SC200D 1 wide angle camera with Gatan Microscopy Suite and Digital Micrograph (Version 2.32.888.0) imaging software. Virions were measured using ImageJ software [31 (link)] (Version 1.8.0_112).

Electron microscopy was performed using 400-mesh formvar and carbon copper grids (ProSciTech, Townsville, Australia). Bacteriophage stocks (>10⁷ PFU/mL) were allowed to adsorb to the grid for one minute before excess solution was removed using filter paper. The grids were then negatively stained three times with 2% (w/v) uranyl acetate for 20 seconds. Excess stain was removed by filter paper and grids were air-dried for 20 min before examination under a JEOL JEM-2100 transmission electron microscope (TEM). This was operated at an accelerating voltage of 200 kV and high-resolution digital images were recorded on a Gatan Orius SC200D 1 wide angle camera with Gatan Microscopy Suite and Digital Micrograph (Version 2.32.888.0) imaging software. Viruses were measured using GMS3 software.

Transmission electron microscopy (TEM) was performed using 400-mesh formvar and carbon copper grids (ProSciTech, Townsville, Australia). Bacteriophage stocks (> 10⁷ PFU/ mL) were allowed to adsorb to the grid for one min before excess solution was removed using filter paper. The grids were then negatively stained three times with 2% (w/v) uranyl acetate for 20 s. Excess stain was removed by filter paper and grids were air-dried for 20 min before examination under a JEOL JEM-2100 transmission electron microscope. This was operated at an accelerating voltage of 200 kV and high-resolution digital images were recorded on a Gatan Orius SC200D 1 wide angle camera with Gatan Microscopy Suite and Digital Micrograph (Version 2.32.888.0) imaging software. Virions were measured using ImageJ software [42 (link)] (Version 1.8.0_112).