Cell culture reagents were purchased from Invitrogen unless otherwise mentioned. The human GC cell lines SGC-7901, BGC-823, MNK45, MGC-803 and HGC-27 (GeneChem, Shanghai, China) were grown in RPMI-1640 or Dulbecco’s’ Modified Eagle's Media (DMEM) medium supplemented with 10% heated inactivated foetal bovine serum (FBS), penicillin (100 U/ml) and streptomycin (100 μg/ml) and maintained in a humidified atmosphere with 5% CO2 and 95% air at 37°C.
Hgc 27
Manufactured by Genechem
Sourced in China
The HGC-27 is a high-performance gas chromatograph designed for advanced analytical applications. It features a compact and durable construction, ensuring reliable performance in laboratory environments. The HGC-27 is capable of separating and analyzing a wide range of organic compounds with high precision and sensitivity.
Automatically generated - may contain errors
Lab products found in correlation
Sgc 7901, by Genechem (11 mentions)
Mgc 803, by Genechem (10 mentions)
Ges 1, by Genechem (9 mentions)
Bgc 823, by Genechem (8 mentions)
Mkn 45, by Genechem (8 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions)
Rpmi 1640 medium, by Corning (3 mentions)
Penicillin, by Thermo Fisher Scientific (3 mentions)
Mnk45, by Genechem (2 mentions)
Cell culture reagents, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Clark Bioscience (1 mentions)
Penicillin, by Cytiva (1 mentions)
Streptomycin, by Cytiva (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Hgc 27, by BNCC (1 mentions)
Ges 1, by BNCC (1 mentions)
Baf a1, by Solarbio (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions)
Rpmi 1640 medium, by Solarbio (1 mentions)
N acetyl cysteine (nac), by Beyotime (1 mentions)
15 protocols using hgc 27
1
Culturing Human Gastric Cancer Cell Lines
2
Gastric cancer cell line culture
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The human normal gastric epithelial cell line GES-1 and the GC cell lines AGS, HGC-27, MGC-803, SGC-7901 and BGC-823 were purchased from Genechem (Shanghai, China). GC cell lines with high metastatic potential (MKN-28 M and SGC-7901 M) and corresponding cell lines with low metastatic potential (MKN-28NM and SGC-7901NM) were constructed from the human GC cell lines MKN28 and SGC-7901, respectively, as described previously [3 (link), 4 (link)]. All cells were cultured in RPMI-1640 medium (GIBCO, Carlsbad, CA, USA) supplemented with 10% FBS, 100 U/ml penicillin sodium and 100 μg/ml streptomycin at 37 °C in a 5% CO2 incubator.
3
Mycoplasma-free Gastric Cell Cultivation
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The normal gastric epithelial cell line GES-1 and the GC cell lines AGS, SGC7901, MGC803, and HGC27 were obtained from GeneChem (Shanghai, China). These cells were tested for mycoplasma every month to ensure that they are free of mycoplasma contamination. The method is as follows: the nucleus was stained with DAPI and observed under an oil microscope. Mycoplasma contamination is characterized by radiating or satellite-shaped spots around the nucleus (22 (link)). All cell lines were cultured in RPMI-1640 medium (Corning, Corning, NY, USA), supplemented with 10% fetal bovine serum (FBS) (Clark Bioscience, Richmond, VA, USA), penicillin (100 U/ml), and streptomycin (100 μg/ml) (HyClone, Logan, UT, USA) in a humidified atmosphere at 37°C with 5% CO2. RPMI-1640 medium lacking Asn was customized by Meilun Biotechnology Co. (Dalian, China). For Asn deprivation assays, the cells were plated overnight in complete RPMI-1640 and subsequently transferred into Asn-free medium after a brief wash with phosphate-buffered saline (PBS).
4
Cell Culture of Gastric Cell Lines
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The human immortalized normal gastric mucosa GES-1 and gastric carcinoma MGC803, BGC823, SGC-7901, MKN-45 and HGC27 cell lines were purchased from Shanghai GeneChem Co., Ltd and conserved in the Laboratory of China Medical University Gastrointestinal Oncopathology. The cell lines were cultured in RPMI 1640 (Hyclone, Thermo scientific, USA) supplemented with 10% fetal bovine serum (Hyclone, USA) in a humidified atmosphere containing 5% CO2 at 37 °C.
5
Gastric Cell Line Cultivation Protocol
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The gastric epithelial cell lines GES-1, MGC803, HGC27, AGS, and SGC7901 were sourced from GeneChem (Shanghai, China) and cultivated in RPMI-1640 medium (Corning Life Sciences, Corning, NY, USA) supplemented with 10% (v/v) fetal bovine serum (FBS; Clark Bioscience, Richmond, VA, USA) and 1% (w/v) penicillin plus 1% (w/v) streptomycin (HyClone Laboratories, Logan, UT, USA). All cell lines were maintained at 37 ℃ under a humidified 5% CO2 atmosphere.
6
Gastric Cancer Cell Line Culture
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The human GC cell lines HGC27, BGC823, MKN45, SNU-1 and the normal gastric epithelial cell line GES1 were purchased from GeneChem (Shanghai, China), identified by STR profiling and tested free of mycoplasma contamination. All cell lines were cultured in high glucose DMEM (HyClone, Logan, Utah, USA) supplemented with 10% fetal bovine serum (Gemini, Calabasas, CA, USA) and 1% penicillin–streptomycin (Gibco, Grand Island, NY, USA) in a humidified incubator in 5% CO2 at 37 °C.
7
Gastric Cancer Cell Line Manipulation
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A normal human gastric epithelial cell line (GES-1) and three GC cell lines (HGC-27, AGS and MKN-45) were acquired from BNCC (Beijing, China). MKN-45 and HGC-27 cells were cultured in RPIM-1640 medium, AGS cells in DMEM/F-12 medium and GES-1 cells in DMEM medium. All culture media were purchased from Gibco (United States), and were supplemented with 1% penicillin-streptomycin and 10% fetal bovine serum. Genechem (Shanghai, China) provided the green fluorescent protein-labeled miR-204-3p overexpression lentiviral vector (OE group), miR-204-3p knockdown lentiviral vector (KD group) and empty lentiviral vector (NC group), which were then transfected into HGC-27, MKN-45 and AGS cells using the tool virus user manual as a guide.
8
Gastric Cancer Cell Line Culturing
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Seven GC cell lines (AGS, BGC823, MGC803, MKN45, MKN1, SGC7901, and HGC27), a gastric mucosa cell line (GES-1), and human kidney cell line (HEK293T) were purchased from GeneChem (Shanghai, China). Cells were cultured in RPMI-1640 medium supplemented with 10% FBS at 37 °C in a humidified atmosphere with 5% CO2.
DAPI, Baf-A1, and 3-MA were obtained from Solarbio (Beijing, China). NAC was purchased from Beyotime (Shanghai, China), and 740Y-P (also known as 740YPDGFR) was obtained from MCE (Monmouth Junction, NJ, USA).
DAPI, Baf-A1, and 3-MA were obtained from Solarbio (Beijing, China). NAC was purchased from Beyotime (Shanghai, China), and 740Y-P (also known as 740YPDGFR) was obtained from MCE (Monmouth Junction, NJ, USA).
9
Gastric Cell Lines Culture Protocol
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Five GC cell lines AGS, SGC-7901, BGC-823, MNK-45, HGC-27, and human immortalized normal gastric epithelial cells GES-1 were provided by Genechem Co., Ltd (Shanghai, China). All the cells were maintained and generated in RPMI 1640 (Gibco, USA) supplemented with 10% fetal bovine serum (Hyclone, USA) and cultured at 37°C in 5% CO2 atmosphere. Cells were collected when they reached the platform stage.
10
Culturing Gastric Cell Lines
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The normal human gastric epithelial cell line GES-1 and human GC cell lines BGC823, SGC7901, MKN45, and HGC27 were purchased from Genechem Co., Ltd., Shanghai, China. These cell lines were then preserved in the Gastrointestinal Onco-Pathology Laboratory of China Medical University. Cells were maintained in RPMI1640 medium (Gibco®; Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FBS (Cellmax, Sydney, Australia), 100 units/mL of penicillin, and 100 µg/mL of streptomycin (Gibco®; Thermo Fisher Scientific, Waltham, MA, USA), and grown in a humidified atmosphere with 5% CO2 at 37°C. The culture medium was changed every other day.
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