Log In Sign up
The largest database of trusted experimental protocols

Tlr4 clone sa15 21

Manufactured by BioLegend
Visit Supplier

TLR4 (clone SA15-21) is a recombinant monoclonal antibody that binds to the Toll-like receptor 4 (TLR4) protein. TLR4 is a pattern recognition receptor that plays a crucial role in the innate immune response to pathogen-associated molecular patterns (PAMPs).

Automatically generated - may contain errors

Lab products found in correlation

Lsrii flow cytometer, by BD (1 mentions) Facscanto 2 cell analyzer, by BD (1 mentions) Fcblock anti cd16 cd32, by BioLegend (1 mentions) Facsverse, by BD (1 mentions) Cd5 clone 53 7.3, by BioLegend (1 mentions) Cd19 clone 6d5, by BioLegend (1 mentions) Cd45 clone 30 f11, by BioLegend (1 mentions)

Spelling variants of this product

TLR4 (SA15-21; (3 citations) SA15-21 (3 citations)

2 protocols using tlr4 clone sa15 21

1

Multiparametric Flow Cytometry of Myeloid Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single-cell suspensions of BMDMs or BMDCs were blocked with FcBlock (anti-CD16/CD32; BioLegend) and subsequently stained with fluorescence-labeled mAbs for CD11b, CD11c, Gr-1, Ly6C, TLR4/MD2 (clone MTS510), and TLR4 (clone SA15-21; all BioLegend). Intracellular staining of human and mouse TNF (both BioLegend) and mouse TLR3 (Imgenex) was performed in permeabilized cells according to standard procedures. Flow cytometric measurements were performed on a FACSCantoII cell analyzer and an LSRII flow cytometer (both BD). Data were analyzed with FlowJo software (version 9.8.3, Mac; version 10.0.7, PC; Tree Star).
Westphal A., Cheng W., Yu J., Grassl G., Krautkrämer M., Holst O., Föger N, & Lee K.H. (2017). Lysosomal trafficking regulator Lyst links membrane trafficking to toll-like receptor–mediated inflammatory responses. The Journal of Experimental Medicine, 214(1), 227-244.
+ Open protocol
+ Expand
2

Immune Cell Profiling in Mouse Peritoneum

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peritoneal cavity of mice was flushed with 8 ml of PBS. Cells were pelleted, washed, and re-suspended in FACS buffer (PBS, 5 mM EDTA, 2.5% FBS) for immunostaining and flow cytometric analyses. Titrated amounts of the following antibodies from BioLegend were used: CD45 (clone 30-F11), CD11b (clone M1/70), F4/80 (clone BM8), CD19 (clone 6D5), CD5 (clone 53–7.3) and TLR4 (clone SA15–21). Data were acquired using FACSVerse (BD) and analyzed using FlowJo software.
Ganeshan K., Nikkanen J., Man K., Leong Y.A., Sogawa Y., Maschek J.A., Van Ry T., Chagwedera D.N., Cox J.E, & Chawla A. (2019). Energetic trade-offs and hypometabolic states promote disease tolerance. Cell, 177(2), 399-413.e12.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!