Ppk gal4

Manufactured by Bloomington Drosophila Stock Center

Sourced in United States

Ppk-Gal4 is a genetic tool used in Drosophila research. It is a Gal4 driver line that expresses the Gal4 transcriptional activator under the control of the pickpocket (ppk) gene promoter, which is active in a specific subset of sensory neurons in the Drosophila nervous system.

Automatically generated - may contain errors

Lab products found in correlation

10 protocols using ppk gal4

Drosophila Cell Death Reporter Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

pUAS-HA-CD:UPRT-attB constructs (containing either a N-terminal HA-tagged S. cerevisiae S.c. CD:UPRT fusion gene or a Drosophila codon-optimized CD:UPRT fusion gene) were used to generate second and third chromosome UAS-CD:UPRT lines for both the S.c.CD:UPRT and optimized CD:UPRT. The following Gal4 lines were obtained from the Bloomington Drosophila Stock Center: Act5C-Gal4 (#25374), GMR12B08-Gal4 (#48489), ppk-Gal4 (#32078 and #32079), MB247-Gal4 (#50742), Canton-S-iso2B (#9514), da-Gal4; da-Gal4 (#55849), GMR32C12-Gal4 (#49708) and 10XUAS-IVS-mCD8-GFP (#32185). TH-Gal4 was provided by F. Wolf.

Hida N., Aboukilila M.Y., Burow D.A., Paul R., Greenberg M.M., Fazio M., Beasley S., Spitale R.C, & Cleary M.D. (2017). EC-tagging allows cell type-specific RNA analysis. Nucleic Acids Research, 45(15), e138.

+ Open protocol

+ Expand

Drosophila Neurodegeneration Genetics

Check if the same lab product or an alternative is used in the 5 most similar protocols

All flies were maintained at 25°C and 60% humidity. The following lines were obtained from Bloomington Drosophila Stock Center (BL) (USA): w1118 (BL 3605), elav-GAL4 (BL 8760), ppk-GAL4 (32078), UAS-ATXN3tr-27Q (BL 8149), UAS-ATXN3tr-78Q (BL 8141), UAS-NFATRNAi (BL 51422), UAS-CrebBRNAi (BL 63681), UAS-CamRNAi (BL 34609), UAS-CanA-14FRNAi (BL 58249), UAS-CanBRNAi (BL 27307), UAS-CaMKIIRNAi (BL 35362), UAS-Pka-C1RNAi (BL 31599), UAS-Pkc53ERNAi (BL 55864), UAS-CalpARNAi (BL 29455), UAS-CalpBRNAi (BL 25963), 109(2)80-GAL4 (BL 8769), UAS-CBP.wt-v5 (BL 32573), UAS-CBP.F2161A-v5 (BL 32574), UAS-HDAC1RNAi (BL 33725), UAS-HDAC3RNAi (BL 34778), UAS-HDAC4RNAi (BL 34744), and UAS-RedStinger (UAS-NLS-DsRed, BL 8546). UAS-Impα3RNAi (106249KK), UAS-IP3RRNAi (106982KK), UAS-CBPRNAi (102885KK), and UAS-CaMKIRNAi (101380KK) were obtained from Vienna Drosophila RNAi Centre (VDRC) (Austria). UAS-CD4-tdGFP, ppk^1a-GAL4, Gene-Switch (GS)-ppk^1a-GAL4, UAS-Htt-152Q-eGFP, and ppk-CD4-tdTom were provided by Yuh Nung Jan (University of California, San Francisco, USA). To activate gene switch-mediated transcription of GAL4, adult flies were fed food with RU486 (100 μM RU486 dissolved in 80% ethanol), according to each experimental condition.

Cho J.H., Jo M.G., Kim E.S., Lee N.Y., Kim S.H., Chung C.G., Park J.H, & Lee S.B. (2022). CBP-Mediated Acetylation of Importin α Mediates Calcium-Dependent Nucleocytoplasmic Transport of Selective Proteins in Drosophila Neurons. Molecules and Cells, 45(11), 855-867.

+ Open protocol

+ Expand

Genetic Manipulation of Neuronal Microtubules

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following alleles and transgenic lines were used: the protein null allele nudE^39A (Wainman et al., 2009 (link)) and Df(3L)BSC673 (Bloomington Stock Center, Bloomington, IN, USA) were used to eliminate NudE; γTub23C^A15-2 and γTub23C^A14-9 (Bloomington Stock Center, Bloomington, IN, USA) were utilized to reduce γ-tubulin activity; UAS-nudE^{wild type}, UAS-nudE^ΔC, nudE^{genomic-wild-type}, nudE^genomic-ΔC and nudE^{genomic-E113A,R124A} were generated in this study (see above for details on their construction); ppk-CD4–GFP (Han et al., 2011 (link)) and ppk-EB1–GFP (this study) were used to visualize neuronal morphology and microtubule polarity, respectively; UAS-Lis1 (Bloomington Stock Center, Bloomington, IN, USA); ppk-Gal4 was used to express UAS-Nod–GFP (Bloomington Stock Center, Bloomington, IN, USA) and UAS-Cherry–nudE (this study), either alone or in combination with UAS-ManII–GFP (Ye et al., 2007 (link)); to characterize Golgi outpost localization in control and nudE⁻ neurons, UAS-ManII–GFP was expressed in combination with UAS-mCD8–GFP using Gal4⁴⁷⁷.

Arthur A.L., Yang S.Z., Abellaneda A.M, & Wildonger J. (2015). Dendrite arborization requires the dynein cofactor NudE. Journal of Cell Science, 128(11), 2191-2201.

+ Open protocol

+ Expand

Imaging Drosophila Class IV da Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fly lines were obtained from the Bloomington Drosophila Stock Center (Bloomington, IN) and through generous gifts from Damon Clark (Yale University) and Fernando Vonhoff (University of Maryland, Baltimore County). Fly stocks were maintained at 25°C in a humidity-controlled incubator (60% humidity) on standard apple agar-based food (Archon Scientific, Durham, NC) with 12-h light/dark cycles. Fly crosses were maintained in fly chambers on apple juice agar-based food (mixture of apple agar concentrate, propionic acid, phosphoric acid, and water) with a generous dollop of yeast paste at 25°C and 60% humidity. Larvae 68–72 h after egg laying were used for all imaging experiments. The following fly lines were used to image class IV da neurons: +;20XUAS-GCaMP6f ;+ (Bloomington #42747), +;ppk-Gal4;+ (Bloomington #32078), +; ;ppk-CD4-tdTomato (Bloomington #35845), +;ppk-CD4-tdTomato;+ (Bloomington #35844),+; ;ppk-CD4-tdGFP (Bloomington #35843).

Basak R., Sutradhar S, & Howard J. (2021). Focal laser stimulation of fly nociceptors activates distinct axonal and dendritic Ca2+ signals. Biophysical Journal, 120(15), 3222-3233.

+ Open protocol

+ Expand

Genetic Toolkit for Drosophila Sensory Neuron Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

Strains w¹¹¹⁸, ppk19^MI02888 (#36434), ppk19^MB05382 (#25293), ppk-gal4, and ppk19-RNAi (#58203, #25887) were sourced from the Bloomington Drosophila Stock Center, and ppk30-RNAi (#105896) from the Vienna Drosophila Resource Center. ppk1^Δ16 and ppk26^Δ11 were gifted by Yu Nung Jan (UCSF). UAS-ppk19 transgenic flies were generated by injecting UAS-ppk19 vector into phiC31 attP-bearing eggs at the Korea Drosophila Resource Center (KDRC).

Jang W., Lim J.Y., Kang S., Kim M., Hwang S.W, & Kim C. (2022). Drosophila ppk19 encodes a proton-gated and mechanosensitive ion channel. Scientific Reports, 12, 18346.

+ Open protocol

+ Expand

Drosophila Genetic Manipulation Toolkit

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fly strains were maintained on a standard diet at 25 ℃, 12 light:12 dark cycles and 70% humidity. Actin-GeneSwitch (#9381), Dilp2-Gal4 (#37516), UAS-dSec16 RNAi (#53917), Ok107-Gal4 (#854), 104y-Gal4 (#81014), Dsk-Gal4 (#51981), Dh44-Gal4 (#51987), Lk-Gal4 (#51992), OK371-Gal4 (#26160), Chat-Gal4 (#6793), 55D01-Gal4 (#39110), Ppk-Gal4 (#79278) and Pdf-Gal4 (#6899) were purchased from the Bloomington Stock Center.

Zhang R.X., Li S.S., Li A.Q., Liu Z.Y., Neely G.G, & Wang Q.P. (2022). dSec16 Acting in Insulin-like Peptide Producing Cells Controls Energy Homeostasis in Drosophila. Life, 13(1), 81.

+ Open protocol

+ Expand

Drosophila Genetic Toolbox for Mechanosensation

Check if the same lab product or an alternative is used in the 5 most similar protocols

All flies were maintained on standard medium at 23–25℃ in 12:12 light-dark cycles. 20×uas-ivs-gcamp6s (BL42746, BL42749), ppk-gal4 (BL32078, BL32079), uas-cd4-tdTom (BL35837, BL35841), ppk-cd4-tdtom (BL35845), ppk-cd4-tdgfp (BL35842, BL35843) uas-lifeact-rfp (BL58715), Ca-α1D^X10 (BL25141) and Mi{ET1}Ca-α1D^MB06807 (BL25527) were from Bloomington Drosophila Stock Center (BDSC). Gal4-19-12 was from the Jan Lab (UCSF). ppk26-gfp was from the Tracey Lab (Indiana University). Uas-mcherry-jupiter was from the Han Lab (Cornell University). Tmc-gal4, gfp-piezo, piezo^KO, uas-gfp-piezo, ppk26¹ and piezo^KO, ppk^Δ5 and were from Wei Zhang (Tsinghua University). tub-gal4 was from Bing Zhou (Tsinghua University). ctⁱ (THU1309), Ca-α1Dⁱ (THU0766) and the control strains were from Tsinghua Fly Center. Ca-α1D^AR66 was from Daniel Eberl (University of Iowa).

Liu Z., Wu M.H., Wang Q.X., Lin S.Z., Feng X.Q., Li B, & Liang X. (2022). Drosophila mechanical nociceptors preferentially sense localized poking. eLife, 11, e76574.

+ Open protocol

+ Expand

Drosophila Genetics Toolbox Utilization

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following stocks were obtained from Tsinghua RNAi Stock Center: UAS-babo-RNAi (THU5256), UAS- uba1-RNAi (THU2127).
The following stocks were obtained from Bloomington Stock Center (BSC): w1118, ok6-gal4, elav-gal4 (c155), PPK-Gal4, mCD8-GFP, UAS-EcR-RNAi (BSC9327), UAS-EcR-B1^DN (BSC9452), UAS-bsk-RNAi (BSC36643), UAS-mCherry-RNAi (BSC35785).
UAS-tubulin-GFP (Dr. Xing Liang, University of Tsinghua).

Xu W., Kong W., Gao Z., Huang E., Xie W., Wang S, & Rui M. (2023). Establishment of a novel axon pruning model of Drosophila motor neuron. Biology Open, 12(1), bio059535.

+ Open protocol

+ Expand

Drosophila Stock Center Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flies were reared on standard yeast/cornmeal agar medium at 25 °C. The ppk-GAL4 (#32078, #32079), ppk1-RNAi (#29571), TrpA1-RNAi (#31504), duox-RNAi (#32903, #38907), duox mutant line (duox^MI11825, #59037) and Ppk-td-GFP (35843) were from Bloomington Drosophila Stock Center.

Jang W., Baek M., Han Y.S, & Kim C. (2018). Duox mediates ultraviolet injury-induced nociceptive sensitization in Drosophila larvae. Molecular Brain, 11, 16.

+ Open protocol

+ Expand

Drosophila Husbandry and Genetic Manipulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Drosophila larvae and adults of mixed sexes were raised on standard cornmeal medium (Nutri-Fly “Bloomington” formulation) under a 12:12 light/dark cycle at 25 °C with 60% humidity, unless otherwise stated. Fly lines Cg-GAL4 (#7011), elav-GAL4; UAS-Dicer-2 (Dcr-2) (#25750), ppk-GAL4 (#32078), UAS-mCD8::GFP (#5137), UAS-AMPKα-K56R (UAS-AMPKα-DN, #50760), Cg-GAL4 (#7011), and Canton S were obtained from Bloomington Drosophila Stock Center (BDSC; Bloomington, IL). UAS-alc-RNAi(KK) (#109325) and the w¹¹¹⁸ (#60000) genetic background line were procured from Vienna Drosophila Resource Center (VDRC; Vienna, Austria). UAS-alc-RNAi(8057-R2) was obtained from the NIG-Fly stock center (Mishima, Shizuoka, Japan). UAS-alc was a generous gift [19 (link)]. We used a cross to w¹¹¹⁸, the isogenic genetic background of the RNAi line and the genetic background for most fly lines, as controls.

Nagy S., Maurer G.W., Hentze J.L., Rose M., Werge T.M, & Rewitz K. (2018). AMPK signaling linked to the schizophrenia-associated 1q21.1 deletion is required for neuronal and sleep maintenance. PLoS Genetics, 14(12), e1007623.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!