3 aminobenzoic acid

Chloroplatinic acid hexahydrate, 3-aminobenzoic acid (ABA), lead(II) acetate trihydrate, iron(II) sulfate heptahydrate, potassium dioxide, dimethyl sulfoxide (DMSO), silver wire (250 μm in diameter), and platinum wire (76 μm in diameter) were purchased from Sigma (St. Louis, MO, USA). Tungsten wire (100 μm in diameter) was purchased from Alfa Aesar (Ward Hill, MA, USA). Calcium chloride and silver conducting epoxy paste were products of Junsei (Tokyo, Japan) and ASAHI (Tokyo, Japan), respectively. Methyltrimethoxysilane (MTMOS) and (Heptadecafluoro-1,1,2,2-tetrahydrodecyl) trimethoxysilane (17FTMS) were purchased from Fluka (Buchs, Switzerland) and Gelest (Tullytown, PA), respectively. Nitric oxide (99.99% and 87,500 ppm) and nitrogen (99.99%) gases were purchased from Dong-A Scientific (Seoul, Korea). Other solvents and chemicals were analytical-reagent grade. Distilled water of 18.2 MΩ cm resistivity was used for preparing all aqueous solutions.

2, 2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), 2,6-dimethoxyphenol (DMP), guaiacol, toluidine, 3-aminobenzoic acid and phenyldiamine were purchased from Sigma-Aldrich (St. Louis, MO), respectively. All other chemicals and reagents were of analytical grade and were purchased from commercial sources, unless otherwise stated. Reagents for polymerase chain reaction (PCR), Ex-Taq DNA polymerase, the genomic DNA extraction kit, and the pGEM-T easy vector were purchased from Promega (Madison, WI). T4 DNA ligase and restriction enzymes were obtained from New England Biolabs (Beverly, MA). Plasmid pPICZαA and Zeocin were purchased from Invitrogen (Carlsbad, CA).