Polyethylene glycol peg 20 000

Manufactured by Solarbio

Sourced in China

Polyethylene glycol (PEG) 20,000 is a high-molecular-weight polymer commonly used in various laboratory applications. It is a water-soluble, non-ionic, and chemically inert compound.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Solarbio (1 mentions) Sodium acetate, by Solarbio (1 mentions) Ribo off rrna depletion kit, by Vazyme (1 mentions) Plasmid extraction kit, by Omega Bio-Tek (1 mentions) Genomic dna extraction kit, by Omega Bio-Tek (1 mentions) Clonexpress 2 one step cloning kit, by Vazyme (1 mentions)

2 protocols using polyethylene glycol peg 20 000

Chitosan-Mediated Rhoa siRNA Delivery

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FAM-labeled Rhoa siRNA was synthesized by Sangon Biotech (Shanghai) Co., Ltd. (Shanghai, China). The sequences of negative control (NC) and Rhoa siRNA were as follows:
Rhoa siRNA sense: UGGCGGAUAUCGAGGUGGAUGGGAATT
Rhoa siRNA antisense: UUCCCAUCCACCUCGAUAUCCGCCATT
NC sense: UUCUCCGAACGUGUCACGUTT
NC antisense: ACGUGACACGUUCGGAGAATT
Chitosan (CS) was purchased from Solarbio, and its molecular weight (480~776 Da) was measured by matrix-assisted laser desorption time-of-flight mass spectrometry. 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide (EDC), N-hydroxysuccinimide (NHS), arginine (Arg), hydroxylamine, sodium acetate, dimethyl sulfoxide, polyethylene glycol (PEG) 20,000, and deuterated heavy water were from Solarbio. NaAc sodium hydroxide, was from Macklin. Lipofectamine 2000 was from Invitrogen. Monoclonal antibodies to Rhoa and actin were from Cell Signaling Technology and Sangon Biotech (Shanghai, China) Co., Ltd., respectively.

Luo J., Chen J., Liu Y., He Y, & Dong W. (2023). A Novel Form of Arginine-Chitosan as Nanoparticles Efficient for siRNA Delivery into Mouse Leukemia Cells. International Journal of Molecular Sciences, 24(2), 1040.

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Characterization of L. fermentum RC4 Strain

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L. fermentum RC4 was preserved at the China General Microbiological Culture Collection Center (CGMCC NO. 8212). De Man, Rogosa and Sharpe (MRS) agar and broth medium were purchased from Qingdao Hope Bio-Technology Co., Ltd. (Shandong, China), and the Luria-Bertani (LB) medium was purchased from Fuyuan Biotch Co., Ltd. (Shanghai, China). The pET-28a-EGFP plasmid was purchased from Fenghui Biotech Co. Ltd (Hunan, China), and the Stbl3 E. coli competent cells were purchased from TransGen Biotech Co., Ltd. (Beijing, China). Polyethylene glycol (PEG) 20000 was purchased from Solarbio Technology Co., Ltd. (Beijing, China). The genomic DNA extraction kit, plasmid extraction kit, gel recycling kit, and reverse transcription PCR kit were obtained from Omega Bio-tek, Ink. (Georgia, USA). TemplatePrepKit 1.0 kit was obtained from KAPA Biosystems (Massachusetts, USA). ClonExpress II One Step Cloning Kit, and Ribo-off rRNA Depletion kit were obtained from Nanjing Vazyme Biotech Co., Ltd. (Jiangsu, China). HiPure Bacterial RNA Kit was obtained from Magen Biotech Co., Ltd. (Shanghai, China). The nitrite reductase activity assay kit was purchased from Suzhou Comin Biotech Co., Ltd. (Jiangsu, China). The nitrite detection kit was purchased from Nanjing Jiancheng Technology Co., Ltd. (Jiangsu, China). Other reagents are analytical grade.

Fan Q., Xia C., Zeng X., Wu Z., Guo Y., Du Q., Tu M., Liu X, & Pan D. (2024). Effect and potential mechanism of nitrite reductase B on nitrite degradation by Limosilactobacillus fermentum RC4. Current Research in Food Science, 8, 100749.

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