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Ec plan neofluar 40x 1

Manufactured by Zeiss
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Sourced in Japan

The EC Plan-NEOFLUAR 40x/1.3 is a high-quality objective lens from Zeiss. It is designed for use in advanced microscopy applications and provides a magnification of 40x with a numerical aperture of 1.3. The lens is optimized for use with immersion oil and is suitable for a variety of microscopy techniques, including brightfield, phase contrast, and fluorescence imaging.

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Lab products found in correlation

Lsm 710 confocal microscope, by Zeiss (6 mentions) Plan apochromat 63x 1, by Zeiss (3 mentions) Mca2060ga, by Thermo Fisher Scientific (2 mentions) Rabbit complexin 1, by Proteintech (2 mentions) Mouse gfap, by Thermo Fisher Scientific (2 mentions) Mouse nsf 1, by Thermo Fisher Scientific (2 mentions) Guinea pig iba1, by Synaptic Systems (2 mentions) Mouse syt1, by Synaptic Systems (2 mentions) Guinea pig znt3, by Synaptic Systems (2 mentions) Lsm 510 imager z 1 microscope, by Zeiss (2 mentions) Plan apochromat 20x 0.8 objective, by Zeiss (2 mentions) Lsm 700, by Zeiss (2 mentions) Zen 2 lite software, by Zeiss (1 mentions) Axioskop 40, by Zeiss (1 mentions) Axiocam 506, by Zeiss (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Blocking one, by Nacalai Tesque (1 mentions) Fluorodish, by World Precision Instruments (1 mentions) Observer z1 microscope, by Zeiss (1 mentions)

6 protocols using ec plan neofluar 40x 1

1

Quantifying Viral Protein and Synaptic Marker Overlap

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Vibratome sections (100 μm thick) from virus-injected animals were immunolabeled with antibodies against mCherry, which was expressed from the virus, as well as antibodies against the mossy fiber marker, ZnT3. CellProfiler (Version 2.1.0) software was used to quantify the degree of overlap between mCherry and ZnT3. The average amount of overlap was 70%.
Primary antibodies: rat BrdU (AbD Serotec, MCA2060GA, 1:500), rabbit GFP (Invitrogen, A11122, 1:300), rabbit Complexin-1 (Proteintech, 10246–2–AP, 1:300), mouse GFAP (G6171, 1:500), mouse Nsf-1 (MA1–12435, 1:300) and rabbit Syn3 (OSS00018W, 1:300) from Thermo Scientific, guinea pig Iba1 (234 004, 1:500), mouse Syt1 (105 011, 1:300), guinea pig ZnT3 (197 004, 1:1000) from Synaptic Systems.
Secondary antibodies. Antibodies (all obtained from Jackson Laboratory, donkey anti-guinea pig IgG (H+L) ML, #706-225-148 and 706-175-148; donkey anti-mouse IgG (H+L) ML, #715-225-151, 715-165-151, and 715-175-151; donkey anti-rabbit IgG (H+L) ML, #711-225-152, 711-165-152, and 711-175-152 were diluted 1:400. Confocal scans were carried out using a LSM 510 Imager Z.1 microscope (Zeiss) using Plan-Apochromat 63X/1.4 and EC Plan-Neofluar 40X/1.30 oil immersion objective lenses and four excitation laser lines. Tile scans were performed using a LSM 710 confocal microscope (Zeiss) with a Plan-Apochromat 20X/0.8 objective.
Siegert S., Seo J., Kwon E.J., Rudenko A., Cho S., Wang W., Flood Z., Martorell A.J., Ericsson M., Mungenast A.E, & Tsai L.H. (2015). The schizophrenia risk gene product miR-137 alters presynaptic plasticity. Nature neuroscience, 18(7), 1008-1016.
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2

Quantifying Viral Protein and Synaptic Marker Overlap

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Vibratome sections (100 μm thick) from virus-injected animals were immunolabeled with antibodies against mCherry, which was expressed from the virus, as well as antibodies against the mossy fiber marker, ZnT3. CellProfiler (Version 2.1.0) software was used to quantify the degree of overlap between mCherry and ZnT3. The average amount of overlap was 70%.
Primary antibodies: rat BrdU (AbD Serotec, MCA2060GA, 1:500), rabbit GFP (Invitrogen, A11122, 1:300), rabbit Complexin-1 (Proteintech, 10246–2–AP, 1:300), mouse GFAP (G6171, 1:500), mouse Nsf-1 (MA1–12435, 1:300) and rabbit Syn3 (OSS00018W, 1:300) from Thermo Scientific, guinea pig Iba1 (234 004, 1:500), mouse Syt1 (105 011, 1:300), guinea pig ZnT3 (197 004, 1:1000) from Synaptic Systems.
Secondary antibodies. Antibodies (all obtained from Jackson Laboratory, donkey anti-guinea pig IgG (H+L) ML, #706-225-148 and 706-175-148; donkey anti-mouse IgG (H+L) ML, #715-225-151, 715-165-151, and 715-175-151; donkey anti-rabbit IgG (H+L) ML, #711-225-152, 711-165-152, and 711-175-152 were diluted 1:400. Confocal scans were carried out using a LSM 510 Imager Z.1 microscope (Zeiss) using Plan-Apochromat 63X/1.4 and EC Plan-Neofluar 40X/1.30 oil immersion objective lenses and four excitation laser lines. Tile scans were performed using a LSM 710 confocal microscope (Zeiss) with a Plan-Apochromat 20X/0.8 objective.
Siegert S., Seo J., Kwon E.J., Rudenko A., Cho S., Wang W., Flood Z., Martorell A.J., Ericsson M., Mungenast A.E, & Tsai L.H. (2015). The schizophrenia risk gene product miR-137 alters presynaptic plasticity. Nature neuroscience, 18(7), 1008-1016.
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3

Fluorescence Microscopy Imaging Protocol

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The cells used for morphological analysis were imaged using an Achroplan 20x/0.45 objective or an EC Plan-NEOFLUAR 40x/1.3 oil objective on a Zeiss Axioskop 40 fluorescent microscope system with the Axiocam 506 mono high resolution camera and the ZEN 2 lite software from Zeiss.
Patel S., Fok S.Y., Stefen H., Tomanić T., Parić E., Herold R., Brettle M., Djordjevic A, & Fath T. (2017). Functional characterisation of filamentous actin probe expression in neuronal cells. PLoS ONE, 12(11), e0187979.
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4

Immunofluorescence Staining of Cells and Tissues

Cited 1 time
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Immunofluorescence was performed as previously described8 (link)40 (link). Briefly, cultured cells or tissue sections were fixed with MeOH at −20 °C for 2 min, blocked on ice using Blocking One (Nacalai Tesque), and then sequentially incubated with appropriate primary and secondary antibodies at room temperature. DNA was stained with 100 ng/mL 4′-6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich), and stained samples were viewed under an IX83 fluorescence microscope with UPlan FL N 40x/0.75 and PlanApo N 60x/1.42 objective lenses (Olympus, Tokyo, Japan) or an LSM710 confocal microscope with EC Plan-NEO FLUAR 40x/1.3 and Plan APOCHROMAT 63x/1.4 objective lenses (Carl Zeiss, Jena, Germany). Composite figures were prepared using Photoshop CS5.
Kuga T., Kume H., Adachi J., Kawasaki N., Shimizu M., Hoshino I., Matsubara H., Saito Y., Nakayama Y, & Tomonaga T. (2016). Casein kinase 1 is recruited to nuclear speckles by FAM83H and SON. Scientific Reports, 6, 34472.
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5

Evaluating Cell Mechanical Properties via AFM

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Cells were grown on glass-bottomed dishes (FluoroDish, World Precision Instruments Inc.) for 24 h before the addition of 5 ng/ml TGFβ1. AFM force curves were obtained using a Bruker Nanowizard 4A instrument coupled with a Zeiss Observer.Z1 Microscope with LSM5 Exciter laser scanning confocal module and ×40 oil immersion objective lens (Zeiss, EC Plan-NeoFluar 40X/1.3). A nitride tip (Bruker, SAA_SPH-5UM) with a nitride lever was used. All the force curves were analyzed using the JPKSPM Data Processing Software.
Basu A., Paul M.K., Alioscha-Perez M., Grosberg A., Sahli H., Dubinett S.M, & Weiss S. (2022). Statistical parametrization of cell cytoskeleton reveals lung cancer cytoskeletal phenotype with partial EMT signature. Communications Biology, 5, 407.
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6

Breast Cancer Cell Antibody Staining

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Conventional antibody staining of cultured breast cancer cells was described in detail previously [29, 66] . The antibodies used for conventional antibody staining were identical to the antibodies used for the PLA and were used in the same concentration. Pictures were taken with a Zeiss LSM700 confocal laser scanning microscope, using a 40x oil immersion objective (EC Plan-Neofluar 40x/1.3; Carl Zeiss AG).
Ames S., Andring J.T., McKenna R, & Becker H.M. (2020). CAIX forms a transport metabolon with monocarboxylate transporters in human breast cancer cells. Oncogene, 39(8).
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