Agilent 500 mhz dd2 spectrometer

Specific rotations were obtained on a JASCO P-1020 digital polarimeter developed by JASCO Corporation, Tokyo, Japan. UV spectra were carried out on Waters 2487 developed by Waters Corporation, Milford, MA, USA. NMR spectra were recorded on Agilent 500 MHz DD2 spectrometers made by Agilent Technologies Inc., Santa Clara, CA, USA, using tetramethylsilane as an internal standard, and the chemical shifts were recorded in δ values. HRESIMS spectra were obtained on a LTQ Orbitrap XL mass spectrometer made by Thermo Fisher Scientific, Waltham, MA, USA. The compounds were purified by HPLC made by the Waters company, Milford, MA, USA, equipped with a 2998 PDA detector and a C18 column (YMC-Pack ODS-A, 10 × 250 mm, 5 µm, 3 mL/min). Medium-pressure preparative liquid chromatography (MPLC) was performed on a Bona-Agela CHEETAH HP100 made by Beijing Agela Technologies Co., Ltd., Beijing, China. Column chromatography (CC) was performed with silica gel (100−200 mesh, 200−300 mesh, Qingdao Marine Chemical Inc, Qingdao, China) and Sephadex LH-20 (Amersham Biosciences, San Francisco, CA, USA), respectively. LC-MS was recorded in ESI mode on an Acquity UPLC H-Class connected to a SQ Detector 2 mass spectrometer using a BEH C18 column (1.7 µm, 2.1 × 50 mm, 1 mL per minute) constructed by Waters Corporation, Milford, CT, USA.

The UV spectra were recorded on a Hitachi 5430 spectrophotometer (Hitachi Ltd., Tokyo, Japan). The ECD spectra and optical rotations were measured on a JASCO J-715 spectropolarimeter and a JASCOP-1020 digital (JASCO Corporation, Tokyo, Japan) polarimeter, respectively. IR spectra were obtained on a Bruker Tensor-27 (Bruker Corporation, Billerica, MA, USA). spectrophotometer in KBr discs. HRESIMS data were measured on a Thermo Scientific LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). NMR spectra were collected on JEOLJN M-ECP 600 (JEOL Ltd., Tokyo, Japan and Agilent 500 MHz DD2 spectrometers (Agilent Technologies, Palo Alto, CA, USA), and tetramethylsilane was used as an internal standard. Sephadex LH-20 (Amersham Biosciences, NJ, USA) and silica gel (Qingdao Marine Chemical Factory, Qingdao, China) were used as stationary phases in column chromatography. An ODS column (YMC-Pack ODS-A, 10 × 250 mm, 5 μm, 3 mL/min, YMC Co., Ltd., Kyoto, Japan) was used for HPLC.

DNA restriction enzymes were purchased from Transgen Biotech Co., LTD (Beijing, China). Polymerase chain reaction (PCR) was performed using TransStart® Fastpfu Fly DNA Polymerase (Transgen Biotech, Beijing, China). Optical rotations were measured on a P-1020 digital polarimeter (JASCO Corporation, Tokyo, Japan). ECD spectra were recorded on a JASCO J-815 spectropolarimeter (JASCO Corporation). UV spectra were recorded on Waters 2487 (Waters Corporation, Milford, MA, United States). HRESIMS and ESIMS spectra were measured on Thermo Scientific LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific). NMR spectra were recorded on Agilent 500 MHz DD2 spectrometer (Agilent Technologies Inc., Santa Clara, CA, United States). Semi-preparative HPLC was performed using a YMC Pack ODS-A column (250 × 10 mm, 5 μm, 3 ml/min, YMC Co., Ltd., Kyoto, Japan). Column chromatography was performed on silica gel (200–300 mesh, Qingdao Marine Chemical Inc., Qingdao, China) and Sephadex LH-20 (GE Healthcare, Uppsala, Sweden).