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Rneasy and dneasy kits

Manufactured by Qiagen
Sourced in United States

The RNeasy and DNeasy kits are lab equipment designed for purification of RNA and DNA, respectively. The RNeasy kit is used to extract and purify total RNA from various sample types, while the DNeasy kit is used to extract and purify genomic DNA from a wide range of biological samples. Both kits utilize a silica-membrane-based technology to efficiently capture and elute the target molecules.

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2 protocols using rneasy and dneasy kits

1

RNA and DNA Extraction Protocol

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RNeasy and DNeasy kits (Qiagen, Germantown, MD, USA) were used, according to the manufacturer’s protocol, to extract the total RNA and DNA from the midfrontal gyrus of the brains of HIV[−] and HIV[+] patients, as previously described [46 (link)]. Using the same technique, total RNA and DNA were extracted from the parietal cortex, striatum and cerebellum of SIV-infected macaques, as previously described [47 (link),48 (link)].
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2

Polycyclic Aromatic Hydrocarbons Exposure Protocol

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BaP (CAS no. 50-32-8) and DBC (CAS no.191-30-0) were purchased from Midwest Research Institute (Kansas City, MO). Coal tar extract (CTE, SRM 1597a) was purchased from the National Institute of Standards & Technology (Gaithersburg, MD). Trizol®, DNase I, EDTA, Superscript™ III, RNase H, and Quant-iT ™ Picogreen® dsDNA assay kit were purchased from Life Technologies™ (Grand Island, NY). RNeasy and DNeasy kits and SYBR® Green Fluor Mastermix were from Qiagen (Alameda, CA) 12-O-tetradecanoylphorbol-13-acetate (TPA) was purchased from Sigma-Aldrich (St. Louis, MO). Acetone and toluene, HPLC grade was from Fisher Scientific (Pittsburgh, PA).
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