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Akt sc 5298

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Akt (sc-5298) is a primary antibody that recognizes the Akt protein, also known as Protein Kinase B (PKB). Akt is a serine/threonine-specific protein kinase that plays a key role in multiple cellular processes such as glucose metabolism, apoptosis, cell proliferation, and cell migration.

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Lab products found in correlation

Facsaria flow cytometer, by BD (1 mentions) Anti actin a5316, by Merck Group (1 mentions) Horseradish peroxidase conjugated second antibody, by Santa Cruz Biotechnology (1 mentions) Sc 365513, by Santa Cruz Biotechnology (1 mentions) Sc 81433, by Santa Cruz Biotechnology (1 mentions) Sc 47724, by Santa Cruz Biotechnology (1 mentions) Phospho akt sc 7985 r, by Santa Cruz Biotechnology (1 mentions) Taqman one step pcr master mix, by Thermo Fisher Scientific (1 mentions) β actin antibody a5441, by Merck Group (1 mentions) On targetplus short interfering si rnas, by Horizon Discovery (1 mentions)

Spelling variants of this product

Sc-5298 (15 citations) Anti-Akt (sc-5298) (3 citations)

5 protocols using akt sc 5298

1

Single-Cell Phospho-Protein Analysis

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Single-cell suspensions were fixed (4% PFA) and permeabilized (PBS, 0.5% saponin, 10% FBS). Cells were incubated with primary antibodies or respective isotype controls for 30 min at 4 °C. Unconjugated Antibodies to PDK1 (3062), P-Akt (Thr308, 9275 and 2965), Akt (2967 and 9272), pan-P-PKC (9371) from Cell Signaling and Akt (sc-5298) and PKCβ (sc-210), from Santa Cruz Biotechnologies were used. Preimmune rabbit sera or isotype matched mouse Ig were used as a control. FITC-conjugated goat anti-mouse or anti-rabbit Ig was added for 30 min at RT in the dark. Cells were acquired on a FACSAria flow cytometer (BD Biosciences) and analyzed using Cyflogic software. The protein level is quantified as the mean fluorescence intensity (MFI) after subtraction of control Ab MFI.
Picco M.E., Castro M.V., Quezada M.J., Barbero G., Villanueva M.B., Fernández N.B., Kim H, & Lopez-Bergami P. (2019). STAT3 enhances the constitutive activity of AGC kinases in melanoma by transactivating PDK1. Cell & Bioscience, 9, 3.
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2

Cellular Signaling Pathway Antibody Panel

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All chemicals were obtained from Sigma-Aldrich Company (St. Louis, MO, USA). The antibody for p16 (Sc-1661), p21 (Sc-6246), p27 (Sc-1641), MMP-2 (sc-10736), NFκB (Sc-109), IκB (Sc-847), ubiquitin (Sc-8017), Ras (Sc-32), RhoA (Sc-418), RhoB (Sc-8048), CDC42 (Sc-87), VEGF (Sc-4570), and AKT (Sc-5298) were purchased from Santa Cruz Biotech (Santa Cruz, CA, USA). The antibodies for FAK (CST#3285), PI-3K (CST#4255), phosphorylated AKT (CST#9275,), phosphorylated FAk (CST#3281), and integrin β3 (CST#4702) were obtained from Cell Signaling Technology (Beverly, MA, USA). Anti-actin (A-5316,) was purchased from Sigma-Aldrich Company.
Chen C.P., Wu Y.L., Chan K.C., Ho H.H., Wang C.J, & Hsu L.S. (2022). Mulberry polyphenols ameliorate atherogenic migration and proliferation by degradation of K-Ras and downregulation of its signals in vascular smooth muscle cell. International Journal of Medical Sciences, 19(10), 1557-1566.
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3

Western Blot Analysis of HepG2 Cells

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After treatment, HepG2 cells were washed with ice-cold PBS and cellular protein was collected by scraping the cells into 50 μL protein extraction buffer, after which western blotting was carried out as described [27 (link)]. Membranes were probed with the antibody against SREBP-1c (sc-365,513), protein kinase B (Akt, sc-5298), phosphorylated protein kinase B (p-Akts473, sc-81,433), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (sc-47,724) (all purchased from Santa Cruz Biotechnology), followed by incubation with horseradish peroxidase-conjugated second antibody. Immunoreactive bands were visualized by the enhanced chemiluminescence detection system.
Bai X.P., Dong F., Yang G.H, & Zhang L. (2019). Influences of sterol regulatory element binding protein-1c silencing on glucose production in HepG2 cells treated with free fatty acid. Lipids in Health and Disease, 18, 89.
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4

Quantification of Signaling Pathway Proteins

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Western blotting was performed as previously described [24 (link)]. The primary antibodies used in this study were rabbit anti-IGF-1R (9750s), phospho-IGF-1R (3021s), ERK (9102s), phospho-ERK (9101s) (1 : 1000 each; Cell Signaling Technology, Danvers, MA); AKT (sc-5298) and phospho-AKT (sc-7985-R) (1 : 1000 each; Santa Cruz Biotechnology); and mouse anti-γH2AX (phosphorS139; 1 : 500; Abcam, San Francisco, CA, USA).
Wang Z., Liu G., Mao J., Xie M., Zhao M., Guo X., Liang S., Li H., Li X, & Wang R. (2019). IGF-1R Inhibition Suppresses Cell Proliferation and Increases Radiosensitivity in Nasopharyngeal Carcinoma Cells. Mediators of Inflammation, 2019, 5497467.
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5

Cellular Signaling Pathway Analysis

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NGF (SC-365944), LOX (SC-373995), p85 (SC-1637), and Akt (SC-5298) antibodies were purchased from Santa Cruz (Biotechnology, CA, USA). The total mTOR (2983S) and phosphorylated forms of p85 (4228S), Akt (4060S), and mTOR (5536S) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). β-Actin (a5441) antibody was purchased from Sigma (Cambridge, MA, USA). All ON-TARGETplus short interfering (si)RNAs were obtained from Dharmacon (Lafayette, CO, USA). Quantitative polymerase chain reaction (qPCR) primers and probes, as well as Taqman® One-Step PCR Master Mix, were supplied by Applied Biosystems (Foster City, CA, USA). Recombinant human NGF was obtained from PerpoTech (Rocky Hill, NJ, USA). All other chemicals used in this study were supplied by Sigma-Aldrich (St. Louis, MO, USA).
Tzeng H.E., Lin S.L., Thadevoos L.A., Lien M.Y., Yang W.H., Ko C.Y., Lin C.Y., Huang Y.W., Liu J.F., Fong Y.C., Chen H.T, & Tang C.H. (2021). Nerve growth factor promotes lysyl oxidase-dependent chondrosarcoma cell metastasis by suppressing miR-149-5p synthesis. Cell Death & Disease, 12(12), 1101.
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