B6 cg tg cag dsred mst 1nagy j

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The B6.Cg-Tg(CAG-DsRed*MST)1Nagy/J is a transgenic mouse line that expresses the DsRed fluorescent protein under the control of the CAG promoter. This line can be used to visualize DsRed-expressing cells and tissues.

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Close spelling variants of this product

Tg(CAG-DsRed*MST)1Nagy/J (7 citations) B6.Cg-Tg(CAG-DsRed*MST)1Nagy/J mice (6 citations) CAG-DsRed (2 citations)

8 protocols using b6 cg tg cag dsred mst 1nagy j

Transgenic Mouse Models Evaluation

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C57Bl/6, OT1 Transgenic (C57Bl/6-Tg(TcraTcrb)1100Mjb/J),DsRed (B6.Cg-Tg(CAG-DsRed*MST)1Nagy/J), and TLR7−/− (B6.129S1-Tlr7tm1Flv/J) mice were purchased from Jackson Laboratories. Animal procedures were approved by the University of Florida Institutional Animal Care and Use Committee (UFIACUC201607966).

Grippin A.J., Wummer B., Wildes T., Dyson K., Trivedi V., Yang C., Sebastian M., Mendez-Gomez H., Padala S., Grubb M., Fillingim M., Monsalve A., Sayour E.J., Dobson J, & Mitchell D.A. (2019). Dendritic Cell-Activating Magnetic Nanoparticles Enable Early Prediction of Anti-Tumor Response with Magnetic Resonance Imaging. ACS nano, 13(12), 13884-13898.

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Generating Transgenic Fluorescent Mice

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C57BL/6 Ctns^−/− mice were provided by Dr. Antignac (Inserm U983, Paris, France). DsRed-transgenic mice (B6.Cg-Tg(CAG-DsRed*MST)1Nagy/J (Jackson Laboratory) were cross-bred with C57BL/6 Ctns^−/− mice to produce transgenic DsRed Ctns^−/− mice constitutively expressing the DsRed fluorescent protein under the control of the chicken β–actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer as previously described [8 (link)]. All strains were bred continuously at University of California, San Diego (UCSD) including wild-type C57BL/6 mice and eGFP-transgenic mice (C57BL/6-Tg(ACTB-EGFP)1Osb/J, Jackson Laboratory). All protocols were approved by the AAALAC-Accredited Institutional Animal Care and Use Committee of UCSD.

Naphade S., Sharma J., Chevronnay H.P., Shook M.A., Yeagy B.A., Rocca C.J., Ur S.N., Lau A.J., Courtoy P.J, & Cherqui S. (2015). Lysosomal cross-correction by hematopoietic stem cell-derived macrophages via tunneling nanotubes. Stem cells (Dayton, Ohio), 33(1), 301-309.

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Transgenic Mouse Model Protocols

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C57Bl/6 (MHC H2b haplotype) mice were purchased from Charles River Laboratories (Calco, Italy). Red fluorescent protein-transgenic (RFP-Tg) mice (B6.Cg-Tg(CAG-DsRed*MST)1Nagy/J) were purchased from The Jackson Laboratory (Bar Harbor, MA, USA). We used female and male mice between 5 and 8 weeks of age. Mice were bred and maintained at the Animal Facility of “IRCCS Azienda Ospedaliera Universitaria San Martino – IST Istituto Nazionale per la Ricerca sul Cancro”. All animal procedures were approved by the“IRCCS Azienda Ospedaliera Universitaria San Martino – IST Istituto Nazionale per la Ricerca sul Cancro” Ethical Committee and performed in accordance with the national current regulations regarding the protection of animals used for scientific purpose (D. Lgs. 4 marzo 2014, n. 26, legislative transposition of Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes).

Lo Sicco C., Tasso R., Reverberi D., Cilli M., Pfeffer U, & Cancedda R. (2015). Identification of a New Cell Population Constitutively Circulating in Healthy Conditions and Endowed with a Homing Ability Toward Injured Sites. Scientific Reports, 5, 16574.

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Mouse Strains for Immunological Studies

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B6.CD45.2 (C57BL/6J; Thy1.2, CD45.2), B6.CD45.1 (B6.SJL-Ptprc^a Pepc^b/BoyJ, Thy1.2, CD45.1), B6.CD11c-YFP (B6.Cg-Tg(Itgax-Venus)1Mnz/J), and DsRed (B6.Cg-Tg(CAG-DsRed*MST)1Nagy/J) were purchased from The Jackson Laboratory (Jax), B6.CD45.1 (B6.SJL-Ptprc^aPepc^b/BoyCrCrl) from Charles River. OT-I mice (C57BL/6-Tg[TcraTcrb]1100Mjb/J; CD45.2) were obtained from Jax and maintained on a Rag^−/− Thy1.1 or Rag^−/− dsRed background. F1.Act-mOVA mice were generated by breeding BALB/cJ mice with B6.Act-OVA (C57BL/6-Tg(CAG-OVAL)916Jen/J). B6.ltbr^−/− mice were obtained from Yang-Xin Fu (previously at the Univ. of Chicago) and maintained at the University of Pittsburgh animal facility. Mice were maintained under SPF conditions. Both male and female mice were used except that female recipient were avoided because of surgical constraints. All mouse work was performed in compliance with ethical regulations and was approved by the Institutional Animal Care and Use Committee of the University of Pittsburgh.

Abou-Daya K.I., Tieu R., Zhao D., Rammal R., Sacirbegovic F., Williams A.L., Shlomchik W.D., Oberbarnscheidt M.H, & Lakkis F.G. (2021). Resident memory T cells form during persistent antigen exposure leading to allograft rejection. Science immunology, 6(57), eabc8122.

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Genetic Manipulation of Mouse Immune Cell Lineages

Cited 1 time

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Clever-1 KO mice with a mixed C57BL/6N and 129SvJ background were generated as previously described (10 (link)) and used with their WT controls (both sexes in a randomized fashion) at an age between 6 and 12 weeks. DsRed mice [B6.Cg-Tg(CAG-DsRed*MST)1Nagy/J] carrying a red fluorescent reporter protein as a transgene were from the Jackson Laboratory (stock number 006051). Balb/C mice were from Janvier and Charles River, CD11c⁺-YFP⁺ mice from Jackson and Kikume mice (14 (link)) from Prof. Masayuki Miyasaka, Osaka University. All animal experiments were approved by The Finnish Act on Animal Experimentation (62/2006); animal license number 5762/04.10.07/2017 and 12537/2020).

Tadayon S., Dunkel J., Takeda A., Eichin D., Virtakoivu R., Elima K., Jalkanen S, & Hollmén M. (2021). Lymphatic Endothelial Cell Activation and Dendritic Cell Transmigration Is Modified by Genetic Deletion of Clever-1. Frontiers in Immunology, 12, 602122.

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Generation of Compound Transgenic Mice

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All mouse studies were conducted with approval by the Institutional Animal Care and Use Committee of the Children’s Hospital of Philadelphia. The CD-1 mice and CD-1 Nude mice (Crl:CD-1Foxn1^nu) were purchased from Charles River Laboratories International, Inc. (Wilmington, MA). The RFP transgenic mice that ubiquitously express red fluorescence protein (RFP) (B6.Cg-Tg(CAG-DsRed*MST)1Nagy/J) were purchased from the Jackson Laboratory (Bar Harbor, ME). The GFP transgenic mice that ubiquitously express enhanced green fluorescence protein (EGFP) (H2K-eGFP) have been established previously [23 (link)]. Scleraxis-GFP reporter (Scx-GFP) mice were kindly provided by Dr. R. Schweitzer (Oregon Health & Science University) [24 (link)]. The Scleraxis-GFP reporter mouse were mated with the RFP-mouse to generate the compound transgenic mouse (Scx-GFP;RFP).

Asai S., Otsuru S., Candela M.E., Cantley L., Uchibe K., Hofmann T.J., Zhang K., Wapner K.L., Soslowsky L.J., Horwitz E.M, & Enomoto-Iwamoto M. (2014). Tendon Progenitor Cells in Injured Tendons Have Strong Chondrogenic Potential: The CD105-Negative Subpopulation Induces Chondrogenic Degeneration. Stem cells (Dayton, Ohio), 32(12), 3266-3277.

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Transgenic Mouse Strains for Regulatory T Cell Analysis

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The B6.Foxp3^DTR/GFP (H-2K^b, CD45.2+)(9 (link)) and B6.Foxp3^GFP (H-2K^b, CD45.2+) mice were obtained from Alexander Rudensky and Tim Ley, respectively. Balb/c (H-2K^d, CD45.2+), C57BL/6 (B6; H-2K^b, CD45.1+), DsRED expressing mice (B6.Cg-Tg(CAG-DsRed*MST)1Nagy/J) and p21-/- mice (B6.129S6(Cg)-Cdkn1atm1Led/J) were purchased from the Jackson Laboratory (Bar Harbor, ME). Animal care and euthanasia procedures were approved by the Washington University School of Medicine Animal Studies Committee.

Cooper M.L., Choi J., Karpova D., Vij K., Ritchey J., Schroeder M.A, & DiPersio J.F. (2017). Azacitidine mitigates GvHD via differential effects on the proliferation of T effectors and nTregs in vivo. Journal of immunology (Baltimore, Md. : 1950), 198(9), 3746-3754.

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Generation of Chimeric Mice with RFP+ Bone Marrow

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C57Bl/6 mice were purchased from Charles River Laboratories (Calco, Italy). Red fluorescent protein-transgenic (RFP-Tg) mice (B6.Cg-Tg(CAG-DsRed*MST) 1Nagy/J) were purchased from the Jackson Laboratory (Bar Harbor, MA, USA). We used female and male mice between 6 and 8 weeks of age. Chimeric mice were generated using lethally irradiated C57Bl/6 mice that were hematopoietically reconstituted with red fluorescent protein-positive (RFP^pos) syngeneic bone marrow nucleated cells. Mice were bred and maintained at the Animal Facility of “IRCCS Ospedale Policlinico San Martino.” All animal procedures were approved by the “ IRCCS Ospedale Policlinico San Martino” Ethical Committee and performed in accordance with the national current regulations regarding the protection of animals used for scientific purpose (D. Lgs. 4 March 2014, n. 26, legislative transposition of Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes).

Lo Sicco C., Reverberi D., Villa F., Pfeffer U., Quarto R., Cancedda R, & Tasso R. (2018). Circulating healing (CH) cells expressing BST2 are functionally activated by the injury-regulated systemic factor HGFA. Stem Cell Research & Therapy, 9, 300.

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