From 2020 to 2021, the specimens were collected from leaves, branches, and culms. The samples were kept in plastic bags and taken back to the laboratory after being photographed with a Sony DSC-HX3 digital camera. The fungi were isolated into pure culture based on single spore isolation [25 (link)]. Glass slide specimens were prepared by free-hand slicing with double-sided blades for morphologic observation. Morphological characteristics of ascomata and sporodochia were observed using a dissecting microscope, the NVT-GG (Shanghai Advanced Photoelectric Technology Co. Ltd., Shanghai, China), and photographed with a VS-800C micro-digital camera (Shenzhen Weishen Times Technology Co. Ltd., Shenzhen, China). An Olympus BX43 compound microscope with an Olympus DP22 digital camera was used to observe and photograph the microstructure of asci, ascospores, conidiophores, and conidia. Measurements were performed using Tarosoft® Image Frame Work v.0.9.7 (Tarosoft (R), Nontha Buri, Thailand). Specimens were deposited at the Herbarium of Sichuan Agricultural University, Chengdu, China (SICAU), and pure cultures were deposited at the Culture Collection in Sichuan Agricultural University (SICAUCC).
Dp22 digital camera
Manufactured by Olympus
Sourced in Japan, Germany
The DP22 is a digital camera designed for laboratory use. It features a high-resolution image sensor and advanced image processing capabilities to capture detailed images. The camera is compatible with a variety of microscopes and imaging systems, allowing for seamless integration into various research and analysis workflows.
Automatically generated - may contain errors
Lab products found in correlation
Bx53 microscope, by Olympus (2 mentions)
Bx43 compound microscope, by Olympus (1 mentions)
Sz61 stereomicroscope, by Olympus (1 mentions)
Triglyceride reagents, by Asan Pharmaceutical (1 mentions)
Bx41 microscope, by Olympus (1 mentions)
Prism v9, by GraphPad (1 mentions)
Szx7 microscope, by Olympus (1 mentions)
Ultraview universal dab detection, by Roche (1 mentions)
Liquid dab substrate chromogen system, by Agilent Technologies (1 mentions)
Cx41 microscope, by Olympus (1 mentions)
Ultravision lp large volume detection system hrp polymer, by Thermo Fisher Scientific (1 mentions)
Anti cd68 antibody clone kp1, by Agilent Technologies (1 mentions)
Benchmark ultra automated immunohistochemical slide staining system, by Roche (1 mentions)
Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (1 mentions)
Fa2h sirna, by Santa Cruz Biotechnology (1 mentions)
Control sirna, by Santa Cruz Biotechnology (1 mentions)
Ckx41 inverted microscope, by Olympus (1 mentions)
Mayer s hematoxylin solution, by Fujifilm (1 mentions)
Paraplast plus embedding medium, by Merck Group (1 mentions)
Eclipse e800 microscope, by Nikon (1 mentions)
16 protocols using dp22 digital camera
1
Fungal Isolation and Morphological Characterization
2
Bacterial Colony Morphology Screening
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Strains were grown overnight in LB with agitation and ampicillin when required. A volume of 3.5 μL of culture was plated on Congo Red Agar (1% tryptone, 1% agar, 40 μL/mL of Congo Red Dye, 0.8 μL/mL of Coomassie Blue) and ampicillin when required. Plates were incubated at 30 °C or 37 °C for 72 h. Pictures of colonies were taken using an Olympus DP22 digital camera and its software with Olympus SZ61 stereomicroscope.
3
Hepatic Lipid and Triglyceride Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liver tissues were fixed in 10% neutral-buffered formalin for 48 h, embedded in paraffin wax and sectioned at 5 μm. For histological examination, sections were stained with hematoxylin and eosin and visualized using light microscope Olympus BX51 (Tokyo, Japan) equipped with Olympus DP22 digital camera (Tokyo, Japan). For the determination of hepatic lipid and TG levels, liver tissue was homogenized in extraction solution (chloroform: methanol = 2:1). Homogenates were mixed with 50mM NaCl and incubated at 4°C overnight, then centrifuged at 1,300g for 10 min at room temperature. The lower phase of extraction solution containing lipids was dried with nitrogen gas and weighed to determine the total lipid contents. The dried lipid pellet was dissolved in 1% triton X-100/PBS and used for TG content measurement using Triglyceride Reagents (Asan Pharmaceutical Co.).
4
Histological Examination of Mouse Kidneys
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The kidneys were removed from each mouse and placed in 10% formalin and were subsequently processed to paraffin blocks. Sections of the kidney tissue were cut at 4 µm and stained with haematoxylin and eosin (H&E) to visualize the host response, and Gomori methenamine silver (GMS) stain to identify the fungal structures. Slides were viewed on an Olympus BX41 microscope and representative images were captured at ×100 and ×200 original magnification using an Olympus DP22 digital camera attached to the microscope.
5
Quantifying Craniofacial Defects in Embryos
6
Histopathological Analysis of Oyster Immunity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Recipient oysters were collected for histopathological analysis at 0 and 72 hpi. Three individuals per family (10 families at 0 and exposed oysters at 72 hpi and 3 families for nonexposed oysters at 72 hpi) were sampled per tank (3 tanks). Following storage in 70% ethanol, whole oysters were embedded in paraffin wax before being serially sectioned to a thickness of 5 μm using a microtome. Tissue sections were collected on polylysine-coated slides and stained with Giemsa (performed by Gribbles Veterinary pathology, New Zealand), which contains a mixture of azure and eosin that variably stain the basic components of the cell pink/purple (e.g., cytoplasm, granules) and methylene blue, which stains the acidic components of the cells blue (e.g., nucleus). The presence of histopathological features was assessed in the mantle and digestive gland of oysters using a light microscope and up to ×1,000 magnification (Olympus BX53 microscope with a DP22 digital camera). Pathological features were categorized as “0” when absent and “1” when present and were converted to a percent presence of a given feature per family, keeping tank replication (n = 3).
7
Microscopic Analysis of Fungal-Infected Wasp Cadavers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At the macroscopic level, the first fungal species had a pale, powdery appearance characteristic of the white muscardine disease caused by Beauveria species [63 (link)], emerging from between segments and joints in the wasps’ cuticle (Figure 1 ). Mycosed wasp specimens infected with the other fungus species developed white to brown, filamentous synnemata, emerging from the hosts’ intersegmental membranes (Figure 2 ), similar to descriptions for hirsutelloid Ophiocordyceps species [64 (link),65 (link)].
For examination of fungal microscopic morphology, fungal samples were harvested from wasp cadavers under a dissecting microscope and mounted directly to microscope slides [66 ]. An Olympus CX41RF compound microscope with an Olympus DP22 digital camera was used to photograph and visually analyse samples.
For examination of fungal microscopic morphology, fungal samples were harvested from wasp cadavers under a dissecting microscope and mounted directly to microscope slides [66 ]. An Olympus CX41RF compound microscope with an Olympus DP22 digital camera was used to photograph and visually analyse samples.
8
Quantifying Angiogenesis in Multiple Myeloma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bone biopsy sections were incubated with mouse anti-human CD138 clone B-A38 (ready to use, Ventana Medical Systems, Tucson, AZ, USA) and the reaction was revealed with polymeric ultraView Universal DAB detection (Ventana Medical Systems) or with rabbit anti-human CD34 (1:100, Santa Cruz Biotechnology, Santa Cruz, CA, USA). After washing, sections were incubated with a secondary antibody (rat anti-Immunoglobulin G horseradish peroxidase (HRP); Millipore, Burlington, MA, USA; 1:250) and the reaction was revealed with a solution of 3,3′-diaminobenzidine tetrahydrochloride (Liquid DAB Substrate Chromogen System, DAKO, Glostrup, Denmark). MVD was evaluated as the number of CD34+ vessels/mm2 in MM patients’ bone biopsies. Images of IHC analyses were captured by a DP22 digital camera (Olympus, Hamburg, Germany) and analyzed with the OLYMPUS Stream software, adjusting tone and contrast to ensure the best image quality.
9
Microstructural Analysis of Cerebral Cavernous Malformations
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For all experiments using microCT quantification of CCM lesion volume, brains were harvested and placed in 4% PFA/PBS. Brains remained in fixative until staining with nondestructive iodine contrast and subsequent microCT imaging performed as previously described (Girard et al., 2016 (link)).
Experiments where brains had observable hemorrhage (e.g., Krit1BECKO; ADAMTS5BEC-GOF) were subject to further analysis. For each mouse, the total blood volume (lesional + extra-lesional) was determine by microCT. Since extra-lesional bleeding cannot be distinguished from blood within caverns on microCT, histological analysis was used. With PECAM immunostaining, ECs were delineated on the largest sections of index lesions identified on microCT. Bleeding was identified on the respective histological images, and the area of extra-lesional blood (not confined by endothelium lining) and the lesional area (including any blood confined within endothelium lined caverns) were measured using the polygon area function of an Olympus DP2-SAL standalone connection kit attached to an Olympus DP22 digital camera mounted on top of an Olympus CX41 microscope.
Tissue processing, imaging, and volumetric quantification were performed in a blinded manner by investigators at the University of Chicago without prior knowledge of genotype or experimental details.
Experiments where brains had observable hemorrhage (e.g., Krit1BECKO; ADAMTS5BEC-GOF) were subject to further analysis. For each mouse, the total blood volume (lesional + extra-lesional) was determine by microCT. Since extra-lesional bleeding cannot be distinguished from blood within caverns on microCT, histological analysis was used. With PECAM immunostaining, ECs were delineated on the largest sections of index lesions identified on microCT. Bleeding was identified on the respective histological images, and the area of extra-lesional blood (not confined by endothelium lining) and the lesional area (including any blood confined within endothelium lined caverns) were measured using the polygon area function of an Olympus DP2-SAL standalone connection kit attached to an Olympus DP22 digital camera mounted on top of an Olympus CX41 microscope.
Tissue processing, imaging, and volumetric quantification were performed in a blinded manner by investigators at the University of Chicago without prior knowledge of genotype or experimental details.
10
Quantification of Immune Markers in Bone Biopsies
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Patient bone biopsies were fixed in formalin at 10%. Once fixed, the samples were embedded in paraffin, so as to allow the cut to the microtome thin sections (3–6 μm). Bone biopsy sections were incubated with polyclonal rabbit Abs against CD38 (1:1.500) (code n. HPA022132, Sigma, Saint Louis, MO) or CD39 (1:70) (code n. 14211-1-AP, Proteintech, Manchester, UK), CD73 (1:1.000), CD31 (1:50), for 60 min at room temperature. Antibodies anti-CD31, CD73 and CD203a were produced in the Lab of one of the authors (FM). The staining was visualized using the UltraVision LP Large Volume Detection System HRP polymer (Thermo Scientific, Erembodegem, Belgium) according to the manifacture's specifications. A sample was considered positive if the target antigen was detected at least in the 50% of cells. Images were captured by DP22 digital camera (Olympus; Hamburg, Germany) and analyzed with the OLYMPUS Stream software, adjusting tone and contrast to ensure the best image quality.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!