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Mir675 3p mimics

Manufactured by RiboBio
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Sourced in China

MiR675-3p-mimics is a laboratory product used for research purposes. It is a synthetic RNA molecule designed to mimic the function of the natural microRNA, miR-675-3p, which is involved in various biological processes. The core function of MiR675-3p-mimics is to serve as a tool for investigating the role and effects of miR-675-3p in experimental settings.

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Lab products found in correlation

Synthetic rna oligonucleotides targeting h19, by RiboBio (1 mentions) Pcdna3.1 h19, by GenePharma (1 mentions) Si h19, by GenePharma (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Opti mem medium, by Thermo Fisher Scientific (1 mentions)

2 protocols using mir675 3p mimics

1

Investigating the Role of H19 and miR675-3p in Cell Lines

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Synthetic RNA oligonucleotides targeting H19 were obtained from RiboBio (Guangzhou, China). The siRNA target sequence was GCGGGTCTGTTTCTTTACT. pcDNA3.1-H19 was purchased from GenePharma (Shanghai, China). miR675-3p-mimics and miR675-3p-inhibitor were obtained from RiboBio (Guangzhou, China). HEK293T cells were transfected with si-H19, pcDNA3.1-H19, miR675-3p-mimics, and miR675-3p-inhibitor for 48 h, respectively. Control cells were transfected with nonspecific, scrambled siRNA.
Hao J., Li C., Lin C., Hao Y., Yu X., Xia Y., Gao F., Jiang Z, & Wang D. (2020). Targeted point mutations of the m6A modification in miR675 using RNA-guided base editing induce cell apoptosis. Bioscience Reports, 40(5), BSR20192933.
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2

Transfection of CRC cells with miRNA and siRNA

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MiR-675-3p mimics, miR-675-3p inhibitors, and miRNA-negative control (miR-NC) were purchased from RiboBio (Guangzhou, China). Specific small interfering RNAs (siRNAs) targeting H19 (si-H19) and negative control (si-NC) were designed and synthesized by Genepharm (Shanghai, China). The CRC cells were transfected with equal concentrations of oligo fragments diluted in Opti-MEM medium (Gibco) by using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) at approximately 80% confluence, according to the manufacturer's instructions.
Weng X., Ma T., Chen Q., Chen B.W., Shan J., Chen W, & Zhi X. (2024). Decreased expression of H19/miR-675 ameliorates hypoxia-induced oxaliplatin resistance in colorectal cancer. Heliyon, 10(5), e27027.
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