Hlb solid phase extraction column

We weighed 0.2 g of lyophilized plant sample powder in a 50 mL centrifuge tube and added 1.8 mL of deionized water to restore the homogenized state of the plant sample; we then added 5.0 g NaCl, 20 mL acetonitrile, vortexed and mixed well. It was then ultrasonicated and extracted for 20 min, centrifuged at 10,000 rpm for 5 min. The extract was taken at 5 mL, nitrogen blown until nearly dry, and the residue was re-dissolved with 1 mL of methanol, then 9 mL of ultrapure water was added and mixed, passed through an HLB solid phase extraction column (150 mg, waters, Milford, MA, USA) pre-treated with 7 mL of methanol and 7 mL of deionized water; the extract was eluted with 7 mL of methanol, then the methanol extract was placed in a water bath at 40 °C, nitrogen blown until nearly dry, and the residue was passed through 1 mL. The residue was re-dissolved with 1 mL of 0.1% formic acid water-acetonitrile solution (1:1, v/v), passed through 0.22 μm nylon filter membrane, and put on the machine for measurement.