Model 1800

The EE of CIP in BLO was estimated using an indirect method based on the finding of free (un-entrapped) CIP [31 (link)]. The specific volume (10 mL) of CIP-BLO dispersion was centrifuged at 12,000 rpm for 20 min and the supernatant separated. Then, the supernatant was diluted with a mixture of methanol and chloroform (1:1), filtered (0.45 µm syringe filter, PTFE/polytetrafluoroethylene, HiMedia, Mumbai, India) and absorbance was analyzed by UV-visible spectrophotometer at 277 nm (Shimadzu, Japan model 1800). The EE is calculated by the given Equation (3).
$$EE \left(\%\right)=\frac{Initial weight of CIP-Weight in supernatant }{Initial weight of CIP}\times 100$$

All antioxidant activities assays were performed in a spectrophotometer UV/VIS (Shimadzu, model 1800, Kioto, JPN). The antioxidant capacity of extracts by free radical DPPH (2,2-diphenyl-1-picrylhydrazyl) was determined according to the procedure of Siripatrawan and Harte [21 ] with minor modifications. Firstly, 25 mg of each film sample was extracted in 3 ml of distilled water, for use as a film extract solution. Then, 0.1 mL of the extract solution was added to 3.9 mL of DPPH methanolic solution (60 μmol.L⁻¹), and kept under dark for 30 min (reaction) and then the absorbance was measured at 515 nm. The results were expressed as a percentage of radical scavenging.
The free radical scavenging by ABTS radical was determined according to Samarth et al. [22 ]. A volume of 88 µL of potassium persulfate (140 mmol/L) was added to 5 mL of ABTS (7 mmol/L). The mixture was stored in an amber bottle in the dark and at room temperature for 16 h. The ABTS radical solution absorbance was adjusted at 0.70 ± 0.05 at the 734 nm in a spectrophotometer. Then, 0.3 mL of film extract solution was added to 3.7 mL of de ABTS radical for 10 min in the dark. After this, the absorbance was measured at 734 nm. The results were expressed in percentage of radical scavenging.