Bapta am b1205

Manufactured by Thermo Fisher Scientific

BAPTA-AM (B1205) is a calcium chelator and intracellular calcium indicator. It is a cell-permeable form of BAPTA, a high-affinity calcium-binding compound. BAPTA-AM can be used to investigate calcium signaling processes within cells.

Automatically generated - may contain errors

Lab products found in correlation

Flagellin, by InvivoGen (1 mentions) Nigericin, by InvivoGen (1 mentions) Cpg oligonucleotide, by InvivoGen (1 mentions) Anti phospho p38 t180 y182, by Cell Signaling Technology (1 mentions) Trizol reagent 15596018, by Thermo Fisher Scientific (1 mentions) Anti chmp4b 13683 1 ap, by Proteintech (1 mentions) Anti β actin sc 1615, by Santa Cruz Biotechnology (1 mentions) Anti caspase 1 ag 20b 0042, by Adipogen (1 mentions) Ultrapure lps, by InvivoGen (1 mentions) Anti ly6c pe, by BioLegend (1 mentions) Adenosine triphosphate (atp), by Merck Group (1 mentions) X tremegene hp dna transfection reagent, by Roche (1 mentions) Anti gapdh 2118, by Cell Signaling Technology (1 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Anti phospho eif2α ab32157, by Abcam (1 mentions) Tunicamycin t7765, by Merck Group (1 mentions) Anti iκb α sc 1643, by Santa Cruz Biotechnology (1 mentions) Anti nf κb sc 8008, by Santa Cruz Biotechnology (1 mentions)

2 protocols using bapta am b1205

Immune Cell Activation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ultrapure LPS, flagellin, nigericin, CpG oligonucleotide, and poly(dA:dT) were purchased from InvivoGen. Silica (MIN-U-SIL 15) was obtained from US Silica. ATP was from Sigma-Aldrich. Pam3Cys was from EMC Microcollections. X-tremeGENE HP DNA transfection reagent was from Roche. Transfection reagent Profect P1 was from Targeting Systems. Acridine Orange (L13159) was from Alfa Aesar. TRIzol reagent (15596018), BAPTA-AM (B1205), and Alexa Fluor 594-labeled zymosan particles (Z23374) were from Thermo Fisher Scientific. Antibodies for immunoblotting include anti-CHMP4B (13683-1-AP) from Proteintech; anti-IKKα (05-536) from Millipore; anti-Mcu (14997), anti-phospho-IkBα (S32), anti-phospho-IKKα/β (S176/180), anti-phospho-p65(S536), anti-p65, anti-phospho-ERK1/2 (T202/Y204), anti-phospho-JNK (T183/Y185), and anti-phospho-p38 (T180/Y182) from Cell Signaling Technology; anti-β-actin (sc-1615) from Santa Cruz Biotechnology; anti-NLRP3 (Cryo-2), anti-Asc (AL177), and anti-caspase-1 (AG-20B-0042) from Adipogen; and anti-IL-1β (AF-401-NA) from R&D Systems. Antibodies for the flow cytometry assay include anti-CD45-PE Cy7 (103114), anti-CD11b-FITC (101206), anti-Ly-6G-PB (127612), and anti-Ly-6C-PE (128008) from BioLegend.

Dong H., Zhao B., Chen J., Liu Z., Li X., Li L, & Wen H. (2022). Mitochondrial calcium uniporter promotes phagocytosis-dependent activation of the NLRP3 inflammasome. Proceedings of the National Academy of Sciences of the United States of America, 119(26), e2123247119.

+ Open protocol

+ Expand

Immunoblotting and Immunofluorescence Antibodies for Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti-PC2 (sc-47734; for immunoblotting), anti-NFκB (sc-8008), and anti-IκBα (sc-1643) antibodies were purchased from Santa Cruz Biotechnology. Anti-PC2 antibody for immunofluorescent imaging was a kind gift from Dr. Stefan Somlo (Yale). Mouse anti-beta-Actin (#3700), rabbit anti-beta-Actin (#4970), anti-CHOP (#2895), anti-cleaved caspase-3 (#9664), and anti-GAPDH (#2118) antibodies were purchased from Cell Signaling Technology. Anti-4-Hydroxynonenal (ab48506), anti-eIF2α (ab50733), anti-phospho-eIF2α (ab32157) antibodies, and kainic acid (ab120100) were purchased from Abcam. Anti-VDAC (PA1-954A) antibody was purchased from Thermo Fisher Scientific. Hydrogen peroxide (H325-100) and BAPTA-AM (B1205) were purchased from Thermo Fisher Scientific. Tunicamycin (T7765), and Dimethyl sulfoxide (DMSO; W387520) were purchased from Sigma-Aldrich.

Brill A.L., Fischer T.T., Walters J.M., Marlier A., Sewanan L.R., Wilson P.C., Johnson E.K., Moeckel G., Cantley L.G., Campbell S.G., Nerbonne J.M., Chung H.J., Robert M.E, & Ehrlich B.E. (2020). Polycystin 2 is increased in disease to protect against stress-induced cell death. Scientific Reports, 10, 386.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!