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Neolynx

Manufactured by Waters Corporation
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Sourced in United Kingdom, United States

Neolynx is a liquid chromatography system designed for high-performance separation and analysis of a wide range of chemical compounds. It features a modular design and advanced technology to deliver reliable and reproducible results.

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Lab products found in correlation

Masslynx, by Waters Corporation (2 mentions) Alliance 2795 lc, by Waters Corporation (2 mentions) Quattro micro, by Waters Corporation (2 mentions) Markerlynx software, by Waters Corporation (1 mentions) Simca p 11, by Sartorius (1 mentions) Xevo tq detector, by Waters Corporation (1 mentions) Tracefinder 4.1 clinical, by Thermo Fisher Scientific (1 mentions) Xcalibur 4, by Thermo Fisher Scientific (1 mentions) Q exactive plus, by Thermo Fisher Scientific (1 mentions) Neobase non derivatized msms kit, by PerkinElmer (1 mentions) Masslynx v4, by Waters Corporation (1 mentions) Quattro ultimaptesi tandem quadrupole mass spectrometer, by Waters Corporation (1 mentions)

7 protocols using neolynx

1

Metabolomic Analysis of Mucosal Tear Samples

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Lipidomics data were processed using MarkerLynx software (Waters, Milford, MA, USA), allowing deconvolution, alignment, and data reduction to give a table of mass and the relative retention time pairs with relative intensities for all the detected peaks. The minimum intensity considered (expressed as % BPI) was set to 10%. The data matrix obtained has been subjected to univariate statistical analysis visualized as a volcano plot using Metaboanalyst 3.0 software.
Metabolomics data were processed using NeoLynx software (Waters, UK), and levels of AAs and ACs were used to build a PLS-DA, using SIMCA-P + 11.0 software (Umetrics AB, Umea, Sweden). The D’Agostino and Pearson omnibus normality test, Student’s t-test, and Mann Whitney U-test were performed for comparisons between clinical groups, using GraphPad Prism (GraphPad software, Inc, La Jolla, CA 92037, USA). Bean plots were obtained by the free online BoxPlotR software http://shiny.chemgrid.org/boxplotr [44 ] The values of p < 0.05 were considered significant. The levels of significantly modulated metabolites in MuS tears were used to perform a Diagnostic power analysis by ROC curve using Metaboanalyst 3.0 software. The 95% of confidence interval was assumed for each test.
Cicalini I., Rossi C., Pieragostino D., Agnifili L., Mastropasqua L., di Ioia M., De Luca G., Onofrj M., Federici L, & Del Boccio P. (2019). Integrated Lipidomics and Metabolomics Analysis of Tears in Multiple Sclerosis: An Insight into Diagnostic Potential of Lacrimal Fluid. International Journal of Molecular Sciences, 20(6), 1265.
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2

Metabolite Profiling of Dried Blood Spots

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Small molecule metabolites of dry blood spots were extracted using underivatized amino acids, carnitine and succinylacetone assay kits (PerkinElmer, Finland) according to the manual. Concentrations of 43 types of amino acid, carnitine and succinylacetone were detected by liquid chromatography and tandem mass spectrometry (Xevo TQ Detector, Waters, Milford, United States) and then analyzed by the software Masslynx (Waters Corporation, Milford, United States) and Neolynx. (Waters Corporation, Milford, United States).
Zhang C., Dha D., Cheng Y., Ma Y., Meng Y., Tse D., Ngawang D., Dekyi P., Jiang T., Shu Y., Cui J., Li J, & Tian Y. (2022). A preliminary investigation of amino acid and acylcarnitine levels in neonates from the Tibet autonomous. Frontiers in Genetics, 13, 941938.
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3

Quantification of Acylcarnitine Species

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The raw data were processed using Neolynx (Waters Corp.). The ratio of ion intensities of acylcarnitine species and its specified deuterated IS was multiplied by the nominal concentration of the IS (5, 1, 1, 1, 2 μmol/L). Concentrations of standards are given in units of μmol/L. Glutarylcarnitine (C5-DC), with a m/z 304, was measured against octanoyl-L-carnitine-d3 (d3-C8) with m/z 305. Propionylcarnitine (C3), with m/z 232, and acetylcarnitine (C2), with m/z 218, were each compared to their own deuterated IS (d3-C2 and d3-C3).
Barzi M., Johnson C.G., Chen T., Rodriguiz R.M., Hemmingsen M., Gonzalez T.J., Rosales A., Beasley J., Peck C.K., Ma Y., Stiles A.R., Wood T.C., Maeso-Diaz R., Diehl A.M., Young S.P., Everitt J.I., Wetsel W.C., Lagor W.R., Bissig-Choisat B., Asokan A., El-Gharbawy A, & Bissig K.D. (2023). Rescue of glutaric aciduria type I in mice by liver-directed therapies. Science translational medicine, 15(692), eadf4086.
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4

Acylcarnitine Metabolite Analysis in Retina

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Acylcarnitine metabolites were extracted from WT and Vldlr−/− (P12) flash frozen retinas using ice-cold methanol. Samples were sonicated, centrifuged and the supernatant was transferred to a fresh tube for nitrogen evaporation. When dry, butanolysis was performed (butanol-HCl, 55°C for 20 min) prior to reconstitution in mobile phase (ACN:H2O 80:20, formic acid 0.05%). Samples were analyzed by liquid chromatography followed by tandem mass spectrophotometry (LC/MS/MS, Alliance 2795 LC and Quattro micro, Waters Corp). Data were recorded in positive electrospray ionization and analyzed with Neolynx (Waters Corp).
Joyal J.S., Sun Y., Gantner M.L., Shao Z., Evans L.P., Saba N., Fredrick T., Burnim S., Kim J.S., Patel G., Juan A.M., Hurst C.G., Hatton C.J., Cui Z., Pierce K.A., Bherer P., Aguilar E., Powner M.B., Vevis K., Boisvert M., Fu Z., Levy E., Fruttiger M., Packard A., Rezende F.A., Maranda B., Sapieha P., Chen J., Friedlander M., Clish C.B, & Smith L.E. (2016). Retinal lipid and glucose metabolism dictates angiogenesis through lipid sensor Ffar1. Nature medicine, 22(4), 439-445.
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5

Quantitative Proteomic Analysis Pipeline

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Data were acquired on the Q-Exactive Plus using Xcalibur 4.1 (Thermo Scientific) and the ChipMate 8.3.3 software (Advion), and processed using TraceFinder™ 4.1 Clinical (Thermo Scientific). Data were acquired on the TQD MS/MS using MassLynx (Waters) and these data were processed using Neolynx™ (Waters). Quantified data were output into excel format, and analysis and visualization were performed using R version 3.4.3 [28 ].
Ruth P., Wang G.X., Boekhoff I., May B., Pfeifer A., Penner R., Korth M., Breer H, & Hofmann F. (1993). Transfected cGMP-dependent protein kinase suppresses calcium transients by inhibition of inositol 1,4,5-trisphosphate production.. Proceedings of the National Academy of Sciences of the United States of America, 90(7), 2623-2627.
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6

Acylcarnitine Metabolite Analysis in Retina

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Acylcarnitine metabolites were extracted from WT and Vldlr−/− (P12) flash frozen retinas using ice-cold methanol. Samples were sonicated, centrifuged and the supernatant was transferred to a fresh tube for nitrogen evaporation. When dry, butanolysis was performed (butanol-HCl, 55°C for 20 min) prior to reconstitution in mobile phase (ACN:H2O 80:20, formic acid 0.05%). Samples were analyzed by liquid chromatography followed by tandem mass spectrophotometry (LC/MS/MS, Alliance 2795 LC and Quattro micro, Waters Corp). Data were recorded in positive electrospray ionization and analyzed with Neolynx (Waters Corp).
Joyal J.S., Sun Y., Gantner M.L., Shao Z., Evans L.P., Saba N., Fredrick T., Burnim S., Kim J.S., Patel G., Juan A.M., Hurst C.G., Hatton C.J., Cui Z., Pierce K.A., Bherer P., Aguilar E., Powner M.B., Vevis K., Boisvert M., Fu Z., Levy E., Fruttiger M., Packard A., Rezende F.A., Maranda B., Sapieha P., Chen J., Friedlander M., Clish C.B, & Smith L.E. (2016). Retinal lipid and glucose metabolism dictates angiogenesis through lipid sensor Ffar1. Nature medicine, 22(4), 439-445.
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7

Quantitative Analysis of AA and AcCs by LC-MS/MS

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AA and AcCs analysis was performed by LC-MS/MS as previously described.58 (link), 59 (link) Cell samples (approximately 1.5 × 106 cells) were extracted with a solution of Ethanol/Water (50 μl, 80 : 20, V:V). The samples were then sonicated and centrifuged (15  600 r.p.m. at 4 °C for 20 min), and the supernatant was recovered. The lysate (7 μl) were added to 100 μl of the stable isotope-labeled IS obtained from the NeoBase Non-derivatized MSMS Kit (Perkin Elmer Life and Analytical Sciences, Turku, Finland). The sample were analysed by direct infusion mass spectrometry using a LC-MS/MS system consisting of an Alliance HT 2795 HPLC Separation Module coupled to a Quattro Ultima Pt ESI tandem quadrupole mass spectrometer (Waters Corporation). The instrument was operated in positive electrospray ionization mode using MassLynx V4.0 Software (Waters Corporation). A detailed description of electrospray ionization mass spectrometry acquisition parameters are available in Supplementary Table S6. Auto data processing was performed using the NeoLynx (Waters Corporation) software.
Ciavardelli D., Rossi C., Barcaroli D., Volpe S., Consalvo A., Zucchelli M., De Cola A., Scavo E., Carollo R., D'Agostino D., Forlì F., D'Aguanno S., Todaro M., Stassi G., Di Ilio C., De Laurenzi V, & Urbani A. (2014). Breast cancer stem cells rely on fermentative glycolysis and are sensitive to 2-deoxyglucose treatment. Cell Death & Disease, 5(7), e1336-.
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