Protein phosphatase inhibitor and protease inhibitor cocktails were purchased from Sigma-Aldrich. Antibodies for ClpX (ab168338, 1:2000), ClpP (ab124822, 1:1000), VDAC (ab34726, 1:1000), α-Synuclein (ab27766, 1:1000), α-Synuclein (ab138501, 1:3000) and α-Synuclein phosphor S129 (ab168381, 1:1000) were from Abcam. GFP (sc-9996, 1:1000), c-Myc (sc-40, 1:1000), α-Synuclein (sc-12767, 1:1000), Enolase (sc-15343, 1:1000) and HSP60 (sc-13115, 1:2000) were from Santa Cruz Biotechnology. β-Actin (A1978, 1:10000) was from Sigma-Aldrich. TH (MAB318, 1:1000) was from Millipore. ClpP (GTX115070, 1:200) was from Genetex. ClpP (NBP1–89557, 1:200) was from Novus. LonP (15440–1-AP, 1:2000), ERAL1 (11478–1-AP, 1:2000), CHCHD3 (25624–1-AP, 1:1000) and WFS1 (11558–1-AP, 1:1000) were from Proteintech. SOD2 (611580, 1:1000) was from BD bioscience. MFN1 (H00055669-M04, 1:1000) was from Abnova. Sirt3 (5490S, 1:1000) was from cell signaling technology.
α synuclein sc 12767
Manufactured by Santa Cruz Biotechnology
α-Synuclein (sc-12767) is a laboratory product offered by Santa Cruz Biotechnology. It is a protein that is commonly used in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Ab124822, by Abcam (2 mentions)
Ab168338, by Abcam (2 mentions)
Ab168381, by Abcam (2 mentions)
C myc sc 40, by Santa Cruz Biotechnology (2 mentions)
Gfp sc 9996, by Santa Cruz Biotechnology (2 mentions)
α synuclein ab27766, by Abcam (2 mentions)
α synuclein ab138501, by Abcam (2 mentions)
Enolase sc 15343, by Santa Cruz Biotechnology (2 mentions)
Hsp60 sc 13115, by Santa Cruz Biotechnology (2 mentions)
Th mab318, by Merck Group (2 mentions)
Clpp gtx115070, by GeneTex (2 mentions)
Clpp nbp1 89557, by Novus Biologicals (2 mentions)
Eral1 11478 1 ap, by Proteintech (2 mentions)
Chchd3 25624 1 ap, by Proteintech (2 mentions)
Sod2 611580, by BD (2 mentions)
Mfn1 h00055669 m04, by Abnova (2 mentions)
Protease inhibitor cocktail, by Merck Group (1 mentions)
β actin a1978, by Merck Group (1 mentions)
Wfs1 11558 1 ap, by Proteintech (1 mentions)
Gapdh sc 365062, by Santa Cruz Biotechnology (1 mentions)
3 protocols using α synuclein sc 12767
1
Protein Phosphatase and Protease Inhibitor Cocktails
2
Antibody panel for mitochondrial proteome
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Protein phosphatase inhibitor and protease inhibitor cocktails were purchased from Sigma–Aldrich. Antibodies for ClpX (ab168338, 1:2000), ClpP (ab124822, 1:1000), VDAC (ab34726, 1:1000), α-Synuclein (ab27766, 1:1000), α-Synuclein (ab138501, 1:3000) and α-Synuclein phosphor S129 (ab168381, 1:1000) were from Abcam. GFP (sc-9996, 1:1000), c-Myc (sc-40, 1:1000), α-Synuclein (sc-12767, 1:1000), Enolase (sc-15343, 1:1000) and HSP60 (sc-13115, 1:2000) were from Santa Cruz Biotechnology. β-Actin (A1978, 1:10000) was from Sigma–Aldrich. TH (MAB318, 1:1000) was from Millipore. ClpP (GTX115070, 1:200) was from Genetex. ClpP (NBP1-89557, 1:200) was from Novus. LonP (15440-1-AP, 1:2000), ERAL1 (11478-1-AP, 1:2000), CHCHD3 (25624-1-AP, 1:1000) and WFS1 (11558-1-AP, 1:1000) were from Proteintech. SOD2 (611580, 1:1000) was from BD bioscience. MFN1 (H00055669-M04, 1:1000) was from Abnova. Sirt3 (5490S, 1:1000) was from cell signaling technology.
3
Immunohistochemistry Staining Protocol
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The IHC staining was performed based on the protocol described in previous study [22 (link)]. The glyceraldehyde-3-phosphate dehydrogenase (GAPDH, sc-365062), α-synuclein (sc-12767) and MAP-2 (sc-74422) antibodies were purchased from Santa Cruz Biotech (Santa Cruz, CA).
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