Vicryl 2 0 suture

The donor mouse was euthanised by cervical dislocation, and tumour was excised. Subsequently, it was cut into 2–3-mm pieces and maintained in Hanks’ Balanced Salt solution (HBSS, Thermofisher) on ice until implantation. The randomly selected recipient animals were anaesthetised with Ketamine and Xylazine (i.p., Ketamine 0.1 μl/g body weight; Xylazine 0.1 μl/g body weight) and placed on a heating pad. The right dorsolateral side was shaved and disinfected with 70% ethanol. Approximately a 5-mm long cut was made through all layers of the skin and separately through the peritoneum. The ovary was gently exteriorised outside of the body cavity and stabilised with a bulldog clamp holding the fat pad. Next, a small incision was made in the fat pad adjacent to the ovary. Surgical glue was applied onto the dissected tumour fragment, which was subsequently pressed into the incision. Alternatively, at this stage, the ES-2 cells were injected into the ovarian bursa, or ID8 cells were injected 12 days before mounting the imaging window. The ovary with the xenograft was gently pushed back inside the body cavity. The peritoneal membrane and the skin were stitched separately with absorbable coated Vicryl 2–0 suture and non-absorbable cotton suture (Ethicon, J&J), respectively. After surgery, animals were placed in the heated recovery unit (Termoplast, Italy).