Flies used in actin quantification and IVEM experiments were maintained on cornmeal/agar/yeast food at room temperature except as noted below. Flies used in all other experiments were maintained on molasses/agar/yeast/malt extract/corn flour/soy flour food at room temperature except as noted below. Stocks used were y1w1 [Bloomington Drosophila Stock Center (BDSC), #1495], Oregon R (BDSC, #5), bmm1 (Grönke et al., 2003 (link)), bmm-GFP (BDSC, #94600, (Zhao et al., 2022 (link)), pxtf01000 (Harvard Exelixis Collection; Thibault et al., 2004 (link)), pxtEY03052 (BDSC, #15620), mdyQX25 (BDSC, #5095), Lsd-2KG00149 (BDSC, #13382), sqh-EYFP-ER (BDSC, #7195; LaJeunesse et al., 2004 (link)), sqh-EYFP-Golgi (BDSC, #7193; LaJeunesse et al., 2004 (link)).
Sqh eyfp golgi
Manufactured by BD
Sourced in Germany
Sqh-EYFP-Golgi is a fluorescent protein-based reagent used for visualizing the Golgi apparatus in live cells. It consists of the enhanced yellow fluorescent protein (EYFP) fused to the Squash (Sqh) protein, which is a well-established marker for the Golgi complex. This product allows researchers to monitor the dynamics and organization of the Golgi apparatus in real-time using fluorescence microscopy.
Automatically generated - may contain errors
2 protocols using sqh eyfp golgi
1
Drosophila Maintenance Protocols for Diverse Experiments
2
Drosophila Genetics and Imaging Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All flies used in experiments were reared and maintained at 25°C on cornmeal fly food with dark/light cycle. For most experiments, white pre-pupae (0 h APF) of the desired genotype were collected and staged at 25°C. Following fly lines that are described in FlyBase and were obtained from the Bloomington Drosophila stock center (BDSC), the Vienna Drosophila Resource Center (VDRC), or the Kyoto Drosophila Genetic and Genomic Resources Center (DGGR), unless noted otherwise were used: UAS-EB1-GFP (BDSC 35512), Ecad-mTomato (BDSC 58789), UAS-Tub-EOS (BDSC 51313), Arm-GFP (BDSC 8555), tubGal4 (BDSC 5138), UAS-Lifeact-GFP (BDSC 35544), FRT42DtubGal80 (II), FRT42Dsspey05252 (DGRC 114436), hsFLP, Tub-Gal4 (BDSC 5138), UAS-GFP (BDSC 5430) sqh-EYFP-Golgi (BDSC 7193), His2avD-mRFP (BDSC 23651), Patronin RNAi (VDRC108927), Ubi-beta-TubGFP (DGRC 109604), UAS-palmKate2-K7 (attP2) (III) from Stefan Luschnig (University of Münster, Germany), GFP-Patronin22H2-N (attP40) (III) (Takeda et al., 2018 (link)), ciGal4 (Croker et al., 2006 (link)), and Ubi-p63E-cnn-RFP (Conduit et al., 2010 (link)).
For clonal analysis patroniney05252 FRT42D mutant positively marked clones were generated using FLP-mediated recombination. Clones were induced by heat-shock of prepua (0 h APF) at 37°C for 10 min in a water bath.
For clonal analysis patroniney05252 FRT42D mutant positively marked clones were generated using FLP-mediated recombination. Clones were induced by heat-shock of prepua (0 h APF) at 37°C for 10 min in a water bath.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!