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5 protocols using zinc chloride

1

Isolation and Characterization of Chinese Fir Tannins

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Chinese fir (Cunninghamia lanceolate), sourcing from Fujian, China, was used as a raw material. Phosphoric acid, phenol, hydrochloric acid, hexamethyleneteramine, formaldehyde, sodium thiosulfate, disodium hydrogen phosphate, monopotassium phosphate, copper sulfate pentahydrate and zinc chloride were purchased from Beijing Chemical Works (Beijing, China). Soluble starch was purchased from Xilong Chemical Co. Ltd. (Shantou, China). Potassium iodide and iodine was purchase from Guangdong Guanghua Sc-tech Co. Ltd. (Shantou, China). Methylene blue was purchase from Tianjin Jinke Fine Chemicals Co. Ltd. (Tianjin, China) All the chemicals were of reagent grade and used without further purification.
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2

Preparation of Protein-Cellulose Nanocomposites

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SPI with 95% protein content was provided by Yuwang Ecological Food Industry Co., Ltd. (Shandong, China). Microcrystalline cellulose [MCC, (C6H10O5)n] was provided by Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). Copper sulfate anhydrous and zinc chloride purchased from Beijing Chemical Works (Beijing, China) were used to prepare Cu NC and Zn NC solutions. Glycerol with 99% purity and sodium hydroxide of analytical grade were purchased from Beijing Chemical Reagents Co., Ltd. (Beijing, China). All solutions were prepared with deionized water.
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3

Synthesis of ZnCl2-EtOH Mixtures

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Ethanol (EtOH, C2H5OH, >99%) and zinc chloride (ZnCl2, >99.9%) were purchased from Beijing Chemical Plant (Beijing, China) and used without further purification. Different compositions of ZnCl2-EtOH mixtures were prepared by weighing for ATR-FTIR analysis. The molar ratios of ZnCl2:EtOH are from 1:14 to 1:3, corresponding to ZnCl2 concentrations from ~6.7 to 25 mol%.
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4

Synthesis and Evaluation of Novel Heterocyclic Compounds

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Terephthalaldehyde (A.R.
Aladdin), acetic anhydride (A.R. Beijing Chemical Works), zinc chloride
(A.R. Beijing Chemical Works), 4-methylpyridine (A.R. Aladdin), 1-bromine
octane (A.R. Aladdin), 1-bromododecane (A.R. Aladdin), 1-bromohexadecane
(A.R. Aladdin), glycoluril (A.R. Aladdin), methanal (A.R. Beijing
Chemical Works), hydrochloric acid (A.R. Beijing Chemical Works),
sulfuric acid (A.R. Beijing Chemical Works), acetone, and ethanol
(A.R. Beijing Chemical Works) were obtained.
MCF-7 (human breast
cancer cell line), HepG2 (human liver cancer cell carcinoma), and
HeLa (human cervical cancer cell line) were bought from the Shanghai
ATCC cell bank issued with the permission of the Human Research Ethics
Committee of the country for manipulation of human’s cells.
The WST-1 kit, culture media (DMEM), and fetal bovine serum (FBS)
were obtained from JIBCO. Phosphate-buffered-saline solution (PBS,
pH = 7.4) was obtained from Beijing Chemical Reagent Company.
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5

Characterization of Candida rugosa Lipase

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Lipase from Candida rugosa (Type VII) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Adenosine 5′-monophosphate (AMP) disodium salt, cytidine 5′-monophosphate (CMP) disodium salt, guanosine 5′-monophosphate (GMP) disodium salt, FeCl3·6H2O, FeCl2·4H2O, N-2-hydroxyethylpiperazine-N′-2-ethyl sulfonic acid (HEPES) and p-nitrophenylpalmitate (p-NPP) were purchased from Aladdin (Shanghai, China). Zinc chloride and NH3·H2O were from Beijing Chemical Works (Beijing, China). Bovine serum albumin (BSA) was from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Milli-Q water was used to prepare all the buffers and solutions. All other reagents and solvents were of analytical grade.
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