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Anti nk1.1 pe cy7

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Anti-NK1.1-PE Cy7 is a fluorochrome-conjugated antibody that binds to the NK1.1 antigen expressed on natural killer cells in mice. It can be used for the identification and enumeration of NK cells in flow cytometry applications.

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Lab products found in correlation

Anti cd4 fitc, by BioLegend (3 mentions) Anti cd19 apc, by BioLegend (3 mentions) Lsrii instrument, by BD (3 mentions) Anti fcr anti cd16 cd32 fc block, by Thermo Fisher Scientific (3 mentions) Anti cd11b pe, by BioLegend (2 mentions) Anti cd8 apc cy7, by BioLegend (2 mentions) 123count ebeads counting beads, by Thermo Fisher Scientific (2 mentions) Anti cd45 percp cy5, by BioLegend (2 mentions) Anti ly6g af700, by Thermo Fisher Scientific (2 mentions) He staining kit, by Beyotime (1 mentions) Anti β actin antibody, by Proteintech (1 mentions) Vascular endothelial growth factor (vegf), by Abcam (1 mentions) E cadherin, by Abcam (1 mentions) Smad2, by Abcam (1 mentions) Mmp 9, by Abcam (1 mentions) Tgf β1, by Abcam (1 mentions) P jnk, by Cell Signaling Technology (1 mentions) P smad2, by Abcam (1 mentions) Anti akt and anti p akt antibodies, by Santa Cruz Biotechnology (1 mentions) Anti cd11c apc, by BioLegend (1 mentions)

Close spelling variants of this product

Anti-NK1.1 (33 citations) NK1.1-PE (20 citations) NK1.1 Pe-Cy7 (5 citations) PE-Cy7-NK1.1 (4 citations) Anti-NK1.1-PE (2 citations) PE/Cy7-anti-NK1.1 mAb (clone PK136, (2 citations)

7 protocols using anti nk1.1 pe cy7

1

Comprehensive Analysis of Tumor Microenvironment

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YFTL was obtained from Changsha Central Hospital (Hunan, China). The HE staining kit was purchased from Beyotime (Jiangsu, China). Antibodies against proliferating cell nuclear antigen (PCNA), p53, MMP-2, MMP-9, E-cadherin, N-cadherin vimentin, VEGF, TGFβ1, Smad2, and p-Smad2 were purchased from Abcam (Cambridge, UK). The cytokine ELISA kits were purchased from Lianke Biotechnology Co. Ltd. (Hangzhou, China). Anti-CD3+ (FITC), anti-CD4+ (PE-Cy5), anti-CD8+ (PE) and anti-NK1.1(PE-Cy7) antibodies were provided by Bio Legend, Inc. (San Diego, CA, USA). Anti-AKT and anti-p-AKT antibodies were provided by Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against ERK1/2, p-ERK1/2, p38, p-p38, JNK, and p-JNK were from Cell Signaling Technologies (Danvers, MA, USA). Anti-β-actin antibodies were from Proteintech Biotechnology (Rocky Hill, USA). Other reagents were purchased from Sigma-Aldrich (St. Louis, Missouri, USA).
Qi Q., Hou Y., Li A., Sun Y., Li S, & Zhao Z. (2019). Yifei Tongluo, a Chinese Herbal Formula, Suppresses Tumor Growth and Metastasis and Exerts Immunomodulatory Effect in Lewis Lung Carcinoma Mice. Molecules, 24(4), 731.
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2

Multiparameter Analysis of Immune Cell Subsets

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Cells were harvested from spleen, mesenteric lymph nodes, bone marrow or thymus. Tissues were crushed into PBS through a 40 micron cell strainer using the back of a 1 mL syringe plunger. Cell preparations were subjected to hypotonic lysis to remove erythrocytes, stained and analyzed using a Fortessa (BD). CD1d(PBS57) tetramer was obtained from the NIH Tetramer Core Facility. Flow cytometry antibodies used in this study were purchased from Biolegend (anti-B220-PacificBlue [clone RA3-6B2], anti-CD107a-Fitc [1D4B], anti-CD11b-AlexaFluorophore 488 [M1/70], anti-CD11c-APC [N418], anti-CD11c-PE/Cy7 [N418], anti-CD25-AlexaFluorophore 488 [PC61], anti-CD44-Bv421 [IM7], anti-CD44-PE/Cy7 [IM7], anti-CD45-Bv711 [30-F11], anti-CD45.1-Bv711 [A20], anti-CD4-APC [RM4-5], anti-CD4-PacificBlue [RM4-5], anti-CD69-PE [H1.2F3], anti-CD8-Bv650 [53-6.7], anti-FoxP3-PE [MF-14], anti-Gr1-PE [RB6-8C5], anti-Gr1-PE/Cy7 [RB6-8C5], anti-IFNγ-Bv421 [XMG1.2], anti-LAG3-Pe [C9B7W], anti-MHC I-A/I-E-Bv510 [M5/114.15.2], anti-NK1.1-FITC [PK136], anti-NK1.1-PE/Cy7 [PK136], anti-PD-1-PE/Cy7 [29F.1A12], Tim3-APC [RMT3-23]) and Affymetrix (anti-CD19-PE [MB19-1], anti-GrzB-Pe/Cy7 [NGZB]).
Tyler P.M., Servos M.M., de Vries R.C., Klebanov B., Kashyap T., Sacham S., Landesman Y., Dougan M, & Dougan S.K. (2017). Clinical dosing regimen of selinexor maintains normal immune homeostasis and T cell effector function in mice: implications for combination with immunotherapy. Molecular cancer therapeutics, 16(3), 428-439.
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3

Immune Cell Phenotyping by Flow Cytometry

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Cells were suspended with FACS buffer (1 mM EDTA, 0.01% sodium azide, 0.1% bovine serum albumin (BSA), 0.02 M phosphate, 0.15 M NaCl, pH 7.2) and then stained for 20 min with anti-FcR/anti-CD16+CD32/Fc Block (eBioscience) and fluorophore-conjugated antibodies: anti-CD45-PerCP Cy5.5 (BioLegend), anti-Ly6G-AF700 (Thermo Fisher Scientific), anti-CD19-APC (BioLegend), anti-CD11b-PE (BioLegend), anti-NK1.1-PE Cy7 (BioLegend), anti-TCR-beta-Pac Blue (BioLegend), anti-CD4-FITC (BioLegend), and anti-CD8-APC/Cy7 (BioLegend). After staining, cells were washed and then fixed with 1% PFA for 30 min. After fixation, cells were washed three times with 200-μl FACS buffer, and then 50 μl of 123count eBeads Counting beads (Thermo Fisher Scientific) were added to allow for quantification of total number of immune cell subtypes following manufacturer’s instructions. Data were acquired on a LSRII instrument (BD Biosciences). Analysis was performed using FlowJo software, version 10.0.8.
Earls R.H., Menees K.B., Chung J., Barber J., Gutekunst C.A., Hazim M.G, & Lee J.K. (2019). Intrastriatal injection of preformed alpha-synuclein fibrils alters central and peripheral immune cell profiles in non-transgenic mice. Journal of Neuroinflammation, 16, 250.
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4

NK Cell Receptor Profiling Protocol

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Splenocytes were suspended with FACS buffer (1 mM EDTA, 0.01% sodium azide, 0.1% Bovine Serum Albumin (BSA), 0.02 M phosphate, 0.15 M NaCl, pH 7.2) and then stained for 20 min with anti-FcR/anti-CD16 + CD32/Fc Block (eBioscience) and the following fluorophore-conjugated antibodies for NK cell receptor profiling: anti-CD19-APC (BioLegend), anti-CD3-Pac Blue (BioLegend), anti-NK1.1-PE Cy7 (BioLegend), anti-NKG2A-PE (BioLegend), anti-CD11b-PerCP (BioLegend), anti-CD27-AF700 (BioLegend), anti-CX3CR1-BV510 (BioLegend), anti-CD107a-APC Cy7 (BioLegend), and anti-NKG2D-FITC (BioLegend). After staining, cells were washed three times with 200 µl FACS buffer. Data were acquired on a LSRII instrument (BD Biosciences). Analysis was performed using FlowJo software, version 10.0.8.
Menees K.B., Earls R.H., Chung J., Jernigan J., Filipov N.M., Carpenter J.M, & Lee J.K. (2021). Sex- and age‐dependent alterations of splenic immune cell profile and NK cell phenotypes and function in C57BL/6J mice. Immunity & Ageing : I & A, 18, 3.
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5

LMWH Modulation of T Cell Phenotypes

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To determine whether LMWH induces CD69 and PD-1 expression on T cells, murine spleen lymphocytes from Balb/c mice were stained with the following antibodies: anti-CD45 BV510 (BioLegend, Cat# 103137), anti-CD3 APC (BioLegend, Cat# 100236), anti-NK1.1 PE-Cy7 (BioLegend, Cat# 108922), anti-CD4 PerCP-Cy5.5 (BioLegend, Cat# 100434), anti-CD8 FITC (BioLegend, Cat# 126606), anti-CD69 BV421 (BD, Cat# 562920), and anti-PD-1 PE (BioLegend, Cat# 135206) antibodies. Nuclei were stained with DAPI (Beyotime, Cat# C1005). Cells were resuspended and blocked with CD16/32 (BioLegend, Cat# 101302) diluted in 1×PBS with 0.2% bovine serum albumin. Cells were detected by multicolor flow cytometry (BD, LSRFortessa) and analyzed by FlowJo (V.10.5.3).
Quan Y., He J., Zou Q., Zhang L., Sun Q., Huang H., Li W., Xie K, & Wei F. (2023). Low molecular weight heparin synergistically enhances the efficacy of adoptive and anti-PD-1-based immunotherapy by increasing lymphocyte infiltration in colorectal cancer. Journal for Immunotherapy of Cancer, 11(8), e007080.
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6

Flow Cytometric Analysis of Lung Cells

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Flow cytometer analysis of the cells evoked by immunization with EVs from P. brasiliensis was performed on the 7th day p.i. Bronchoalveolar cells were harvested and labeled with a panel of monoclonal antibodies (anti-CD4/FITC, anti-CD8a/PE, anti-CD3/PE-Cy5, anti-CD44/Alexa 647, and anti-NK1.1/PE-Cy7) (all from Biolegend Inc., San Diego, CA, USA). Data were acquired on a FACSCanto II (Becton Dickinson, Franklin Lakes, NJ, USA) and analyzed using FlowJo v10 software (TreeStar Inc., Ashland, OR, USA. Limits for the quadrant markers were always set based on the negative population stained with isotype controls. Results were presented as cell number per mL of BAL (×105).
Baltazar L.M., Ribeiro G.F., Freitas G.J., Queiroz-Junior C.M., Fagundes C.T., Chaves-Olórtegui C., Teixeira M.M, & Souza D.G. (2021). Protective Response in Experimental Paracoccidioidomycosis Elicited by Extracellular Vesicles Containing Antigens of Paracoccidioides brasiliensis. Cells, 10(7), 1813.
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7

Immune Cell Profiling by Flow Cytometry

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Splenocytes were suspended with FACS buffer (1 mM EDTA, 0.01% sodium azide, 0.1% Bovine Serum Albumin (BSA), 0.02 M phosphate, 0.15 M NaCl, pH 7.2) and then stained for 20 min with anti-FcR/anti-CD16 + CD32/Fc Block (eBioscience) and the following fluorophore-conjugated antibodies for immune cell profiling: anti-CD45-PerCP Cy5.5 (BioLegend), anti-Ly6G-AF700 (Thermo Fisher Scientific), anti-CD19-APC (BioLegend), anti-CD11b-PE (BioLegend), anti-NK1.1-PE Cy7 (BioLegend), anti-TCR-β-Pac Blue (BioLegend), anti-CD4-FITC (BioLegend), and anti-CD8-APC/Cy7 (BioLegend). After staining, cells were washed three times with 200 µl FACS buffer, and then 50 µl of 123count eBeads Counting beads (Thermo Fisher Scientific) were added to allow for quantification of total number of immune cell subtypes following manufacturer’s instructions. Data were acquired on a LSRII instrument (BD Biosciences). Analysis was performed using FlowJo software, version 10.0.8.
Menees K.B., Earls R.H., Chung J., Jernigan J., Filipov N.M., Carpenter J.M, & Lee J.K. (2021). Sex- and age‐dependent alterations of splenic immune cell profile and NK cell phenotypes and function in C57BL/6J mice. Immunity & Ageing : I & A, 18, 3.
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