Immature and mature LCs were phenotyped using CD1a-APC (BD Pharmingen), langerin-PE (Novocastra), CD86-FITC (BD Pharmingen), CD80-PE (BD Pharmingen), CD83-APC (BD Pharmingen), CD4-AF488 (Biolegend), CD195 (CCR5)-PE (BD Pharmingen) and unlabeled CXCR4 (R&D systems) for which secondary detection with Goat-anti-Mouse-A488 (Invitrogen) was used. HIV-1 infection and transmission samples were stained for CD1a-APC (LC marker), CD3-PerCP (T cell marker), and p24-PE (HIV-1 envelope protein, Beckman Coulter). Immature vaginal LCs were further sorted with a FacsARIA 3 laser sorter (BD Biosciences) after staining for CD1a-APC into CD1a positive and CD1a negative fractions. Samples were analysed using FACSCanto II flow cytometers (BD Biosciences) and data analysis was carried out with FlowJo V10.
Cd3 percp
Manufactured by Beckman Coulter
Sourced in United States
The CD3 PerCP is a flow cytometry reagent used to identify and enumerate T lymphocytes. It contains an antibody conjugated to the PerCP (peridinin-chlorophyll protein) fluorescent dye, which binds to the CD3 surface antigen expressed on T cells. This reagent enables the detection and quantification of T cell populations in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Facscanto 2 flow cytometer, by BD (1 mentions)
Cd80 pe, by BD (1 mentions)
Cd86 fitc, by BD (1 mentions)
Cd4 af488, by BioLegend (1 mentions)
Cd83 apc, by BD (1 mentions)
Cd1a apc, by BD (1 mentions)
Goat anti mouse a488, by Thermo Fisher Scientific (1 mentions)
Cd11b pe, by BD (1 mentions)
Cd56 apc, by BD (1 mentions)
Cd73 pe, by BD (1 mentions)
Cd56 pe, by BD (1 mentions)
Cd14 apc, by BD (1 mentions)
Cd107a fitc, by BD (1 mentions)
Granzyme a fitc, by BD (1 mentions)
Perforin fitc, by BD (1 mentions)
Cd3 pe, by Beckman Coulter (1 mentions)
Cd44 fitc, by Agilent Technologies (1 mentions)
Cd3 percp, by BD (1 mentions)
Cd69 pe, by BD (1 mentions)
Lsrfortessa, by BD (1 mentions)
3 protocols using cd3 percp
1
Phenotypic Analysis of Langerhans Cells
2
Multiparametric Flow Cytometry Analysis
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The anti-human mAbs have been used: CD3 PE (from Beckman Coulter), CD3 PErCP, CD11b PE, CD14 APC, CD56 PE, CD56 APC, CD73 PE, CD107a FITC, Granzyme A FITC, Perforin FITC, pJNK PE (from BD Biosciences), HLA-DR FITC (Dako), CD44 FITC, CD105 FITC, Granzyme B PE, Granzyme K Alexa647 (Immunotools), CD90 APC, COX2 FITC (Cayman chemicals). Each flow cytometric analysis was controlled with isotype-matched mAbs. All flow cytometry-based experiments were performed on FACS Calibur using Cell-Quest Pro Software. Offline data analysis was done on Summit 5.1 software.
3
NK Cell Phenotyping Post-Transplant
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Frozen PBMC and BAL samples were thawed rapidly and the viability determined by trypan blue exclusion. Antibodies used in this study included the following: CD56 Brilliant Violet (BV) 421, CD3 PerCP, KIR3DL1-APC, CD57-FITC (all from Becton Dickinson Biosciences, NJ, USA), NKG2A-PE, NKG2C-AlexaFluor 700 (both from R&D systems), KIR2DL1/2DS1 PECy7, KIR2DL2/2DL3/2DS2 PECy5.5 (all from Beckman Coulter), and KIR3DL2-biotin (courtesy of K.J. Malmberg, Karolinska University Hospital, Stockholm, Sweden) followed by Streptavidin BV 605. There were additional PBMC and BAL samples available for one high-risk recipient at 9 months posttransplant, and further phenotyping was performed by antibodies to CD2-FITC, CD69-PE, HLA-DR PerCP Cy5.5, Ki67 PECy7, CD16 BV 605, and CD103 BV711 (all from Becton Dickinson Biosciences, NJ, USA), ILT2 APC (Beckman Coulter), as well as antibodies to identify NKG2C + NK cells as above (CD3 PerCP, CD56 BV 421, and NKG2C-AlexaFluor 700). All samples also included a viability dye (Live/Dead Fixable Near-IR, Thermofisher). Flow cytometry analysis was performed using a Becton Dickinson LSRFortessa (NJ, USA). Samples were gated on single, live lymphocytes, followed by gating on CD3 -CD56 + and analyzed using FlowJo software (Treestar, San Carlos, USA).
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