Dr bdnf 1 sg quantitect primer assay

Standard AED diazepam (DZP) and Pentylenetetrazol (PTZ) were commercially obtained from Sigma Aldrich (USA). TRIzol^® reagent was purchased from Invitrogen, USA. The primer sets used for QuantiTect SYBR Green dye (Qiagen, Valencia, CA, USA) were as follows: NF-κB: Dr_nfkb1_2_SG QuantiTect Primer Assay (Cat no. QT02498762); BDNF: Dr_bdnf_1_SG QuantiTect Primer Assay (Cat no. QT02125326); eef1a1b: Dr_eef1a1b_2_SG QuantiTect Primer Assay (Cat no. QT02042684); c-Fos: Dr_fos_1_SG QuantiTect Primer Assay (Cat no. QT02103243).

Gene expression for NPY, BDNF and CREB1 were computed using real-time quantitative RT-PCR (Applied Biosystems) using QuantiTect SYRB Green dye (Qiagen, Valencia, CA, United States). All the primer sets were purchased by Qiagen (npy: Dr_npy_1_SG QuantiTect Primer Assay (QT02205763), bdnf: Dr_bdnf_1_SG QuantiTect Primer Assay (QT02125326), creb:Dr_crebbpa_1_SG QuantiTect Primer Assay (QT02197503). Samples were incubated at 95°C for 2 min prior to thermal cycling (40 cycles of 95°C for 5 s and 60°C for 15 s). Relative expression of these three genes were attained by normalizing threshold cycle (Ct) values against Ct value of eef1a1b (housekeeping gene) (2 ^∧ [Ct eef1a1b – Ct Gene of interest]).