C57bl 6 and balb c mice

All mouse experiments were approved by the local ethics committee (approval 2747, Basel Stadt, Switzerland) and performed in accordance with the Swiss federal regulations. C57BL/6 and BALB/c mice were obtained from Janvier Labs (France) and bred in-house at the Department of Biomedicine, University Hospital Basel, Switzerland. The full-body Siglec-E–deficient mouse strain (EKO) was received from A. Varki (University of California, San Diego) and had been previously described (43 (link)). EKO mice were bred in-house and backcrossed to the local C57BL/6 strain in heterozygous pairings for more than nine generations. Siglece^flox/flox mice were generated by Biocytogen as previously described (32 (link)). Itgax^cre (CD11c^cre) mice were provided by D. Finke (University of Basel, Switzerland). Xcr1^cre mice were a gift from T. Kaisho (Wakayama Medical University, Japan) (44 (link)). All animals were housed under specific pathogen–free conditions.

Ten-week-old male and female mice deficient for a single FcγR (FcγRI^–/–, FcγRIIB^–/–, FcγRIII^–/–, FcγRIV^–/–), all activating FcγRs (FcRγ^–/–), or all FcγRs (FcRγ^–/–FcγRIIB^–/–) on C57BL/6 background and FcγRIIB^–/–, FcRγ^–/–, and FcRγ^–/–FcγRIIB^–/– mice on BALB/c background were used in this study. C57BL/6 and BALB/c mice were obtained from Elevage Janvier (Le Genest-Saint-Isle, France). Jeffrey Ravetch (Rockefeller University, NY, United States) provided all FcγR-deficient strains. All strains were bred in-house for several generations. Animals were maintained under specific pathogen-free conditions. The study was approved by the Ethical Committee of the District Government of Lower Franconia.