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Rplpo antibody

Manufactured by Proteintech
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Sourced in United States

The RPLPO antibody is a research-use-only product developed by Proteintech. It is designed to detect the RPLPO protein, which is a component of the large ribosomal subunit. The antibody can be used in various immunoassay applications to study the expression and localization of RPLPO in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Spectramax m5 microplate reader, by Molecular Devices (5 mentions) Elisa maxisorp 96 well plates, by Thermo Fisher Scientific (5 mentions) Amplex red soluble fluorophore, by Thermo Fisher Scientific (5 mentions) Horseradish peroxidase hrp conjugated secondary antibody, by Thermo Fisher Scientific (5 mentions) Amplexultrared soluble fluorophore, by Thermo Fisher Scientific (2 mentions) Aspectramax m5, by Molecular Devices (2 mentions) Akt pathway total and phospho panels, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

RPLPO (3 citations)

7 protocols using rplpo antibody

1

Direct Binding Duplex ELISA Immunoassay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Direct binding duplex ELISAs measured immunoreactivity with results
normalized to large acidic ribosomal protein (RPLPO) [33 (link)]. Immunoreactivity to target proteins was
detected with horseradish peroxidase-conjugated secondary antibody and Amplex
UltraRed soluble fluorophore (Invitrogen, Carlsbad, CA). RPLPO antibody
(Proteintech Group Inc, Chicago, IL) was biotinylated, and its immunoreactivity
was detected with streptavidin-conjugated alkaline phosphatase and the
4-Methylumbelliferyl phosphate (4-MUP) substrate. Fluorescence intensities
(Amplex Red: Ex 565 nm/Em 595 nm; 4-MUP: Ex360/Em450) was measured in a
SpectraMax M5 (Molecular Devices, Sunnyvale, CA). Antibody omission controls
were included. The calculated target protein/RPLPO ratios were used for
inter-group comparisons.
Andreani T., Tong M., Gundogan F., Silbermann E, & de la Monte S.M. (2016). Differential Effects of 3rd Trimester-Equivalent Binge Ethanol and Tobacco-Specific Nitrosamine Ketone Exposures on Brain Insulin Signaling in Adolescence. Journal of diabetes and related disorders, 1(1), 105.
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2

Monoclonal Antibody Generation and Purification

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Pharmaceutical grade ethanol was used in all experiments. The A85G6 and A85E6 monoclonal antibodies to ASPH were generated to human recombinant protein (de la Monte et al., 2009 (link)) and purified over Protein G columns (Healthcare, Piscataway, NJ). Otherwise, antibodies used for duplex ELISAs were purchased from Abcam (Cambridge, MA). RPLPO) antibody was from the Proteintech Group Inc (Chicago, IL). ELISA MaxiSorp 96-well plates were purchased from Nunc (Rochester, NY). Horseradish peroxidase (HRP)-conjugated secondary antibody, Amplex Red soluble fluorophore, and the Akt Pathway Total and Phospho panels were purchased from Invitrogen (Carlsbad, CA). HRP-labeled polymer conjugated secondary antibody was purchased from Dako Corp (Carpinteria, CA). The SpectraMax M5 microplate reader was purchased from Molecular Devices Corp. (Sunnyvale, CA). BCA reagents were from Pierce Chemical Corp. (Rockford, IL). All other fine chemicals, including NNK were purchased from CalBiochem (Carlsbad, CA), Pierce (Rockford, IL), or Sigma (St. Louis, MO).
Re E., Tong M, & de la Monte S.M. (2016). Tobacco Nitrosamine Exposures Contribute to Fetal Alcohol Spectrum Disorder Associated Cerebellar Dysgenesis. International journal of biology, 8(3), 10-21.
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3

Purification and Characterization of ASPH Antibodies

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Pharmaceutical grade ethanol was used in the in vivo experiments. The A85G6 and A85E6 monoclonal antibodies to ASPH and Humbug were generated into human recombinant proteins and purified over Protein G columns (Healthcare, Piscataway, NJ, USA) [34 (link)]. All other primary antibodies used for the duplex ELISAs were purchased from Abcam (Cambridge, MA, USA). RPLPO antibody was sourced from the Proteintech Group Inc (Chicago, IL, USA). ELISA MaxiSorp 96-well plates were purchased from Nunc (Rochester, NY, USA). Horseradish peroxidase (HRP)-conjugated secondary antibody, and Amplex Red soluble fluorophore were purchased from Invitrogen (Carlsbad, CA, USA). The SpectraMax M5 microplate reader was purchased from Molecular Devices Corp. (Sunnyvale, CA, USA). BCA reagents were obtained from Pierce Chemical Corp. (Rockford, IL, USA). All other fine chemicals were purchased from CalBiochem (Carlsbad, CA, USA), Pierce (Rockford, IL, USA), or Sigma (St. Louis, MO, USA).
Tong M., Ziplow J.L., Mark P, & de la Monte S.M. (2022). Dietary Soy Prevents Alcohol-Mediated Neurocognitive Dysfunction and Associated Impairments in Brain Insulin Pathway Signaling in an Adolescent Rat Model. Biomolecules, 12(5), 676.
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4

Monoclonal Antibody Production and Purification

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Pharmaceutical grade ethanol was used in the in vivo experiments. The A85G6 and A85E6 monoclonal antibodies to ASPH were generated to human recombinant protein [46 (link)] and purified over Protein G columns (Healthcare, Piscataway, NJ, USA). Otherwise, antibodies used for duplex ELISAs were purchased from Abcam (Cambridge, MA, USA). RPLPO antibody was from the Proteintech Group Inc. (Chicago, IL, USA). ELISA MaxiSorp 96-well plates were purchased from Nunc (Rochester, NY, USA). Horseradish peroxidase (HRP)-conjugated secondary antibody and Amplex Red soluble fluorophore were purchased from Invitrogen (Carlsbad, CA, USA). The SpectraMax M5 microplate reader was purchased from Molecular Devices Corp. (Sunnyvale, CA, USA). BCA reagents were from Pierce Chemical Corp. (Rockford, IL, USA). All other fine chemicals were purchased from CalBiochem (Carlsbad, CA, USA), Pierce (Rockford, IL, USA) or Sigma (St. Louis, MO, USA).
Tong M., Gonzalez-Navarrete H., Kirchberg T., Gotama B., Yalcin E.B., Kay J, & de la Monte S.M. (2017). Ethanol-Induced White Matter Atrophy Is Associated with Impaired Expression of Aspartyl-Asparaginyl-β-Hydroxylase (ASPH) and Notch Signaling in an Experimental Rat Model. Journal of drug and alcohol research, 6, 236033.
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5

Direct Binding Duplex ELISA Immunoassay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Direct binding duplex ELISAs measured immunoreactivity with results
normalized to large acidic ribosomal protein (RPLPO) [33 (link)]. Immunoreactivity to target proteins was
detected with horseradish peroxidase-conjugated secondary antibody and Amplex
UltraRed soluble fluorophore (Invitrogen, Carlsbad, CA). RPLPO antibody
(Proteintech Group Inc, Chicago, IL) was biotinylated, and its immunoreactivity
was detected with streptavidin-conjugated alkaline phosphatase and the
4-Methylumbelliferyl phosphate (4-MUP) substrate. Fluorescence intensities
(Amplex Red: Ex 565 nm/Em 595 nm; 4-MUP: Ex360/Em450) was measured in a
SpectraMax M5 (Molecular Devices, Sunnyvale, CA). Antibody omission controls
were included. The calculated target protein/RPLPO ratios were used for
inter-group comparisons.
Andreani T., Tong M., Gundogan F., Silbermann E, & de la Monte S.M. (2016). Differential Effects of 3rd Trimester-Equivalent Binge Ethanol and Tobacco-Specific Nitrosamine Ketone Exposures on Brain Insulin Signaling in Adolescence. Journal of diabetes and related disorders, 1(1), 105.
+ Open protocol
+ Expand
6

Monoclonal Antibody Generation and Purification

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Pharmaceutical grade ethanol was used in all experiments. The A85G6 and A85E6 monoclonal antibodies to ASPH were generated to human recombinant protein (de la Monte et al., 2009 (link)) and purified over Protein G columns (Healthcare, Piscataway, NJ). Otherwise, antibodies used for duplex ELISAs were purchased from Abcam (Cambridge, MA). RPLPO) antibody was from the Proteintech Group Inc (Chicago, IL). ELISA MaxiSorp 96-well plates were purchased from Nunc (Rochester, NY). Horseradish peroxidase (HRP)-conjugated secondary antibody, Amplex Red soluble fluorophore, and the Akt Pathway Total and Phospho panels were purchased from Invitrogen (Carlsbad, CA). HRP-labeled polymer conjugated secondary antibody was purchased from Dako Corp (Carpinteria, CA). The SpectraMax M5 microplate reader was purchased from Molecular Devices Corp. (Sunnyvale, CA). BCA reagents were from Pierce Chemical Corp. (Rockford, IL). All other fine chemicals, including NNK were purchased from CalBiochem (Carlsbad, CA), Pierce (Rockford, IL), or Sigma (St. Louis, MO).
Re E., Tong M, & de la Monte S.M. (2016). Tobacco Nitrosamine Exposures Contribute to Fetal Alcohol Spectrum Disorder Associated Cerebellar Dysgenesis. International journal of biology, 8(3), 10-21.
+ Open protocol
+ Expand
7

Monoclonal Antibody Production and Purification

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Pharmaceutical grade ethanol was used in the in vivo experiments. The A85G6 and A85E6 monoclonal antibodies to ASPH were generated to human recombinant protein [46 (link)] and purified over Protein G columns (Healthcare, Piscataway, NJ, USA). Otherwise, antibodies used for duplex ELISAs were purchased from Abcam (Cambridge, MA, USA). RPLPO antibody was from the Proteintech Group Inc. (Chicago, IL, USA). ELISA MaxiSorp 96-well plates were purchased from Nunc (Rochester, NY, USA). Horseradish peroxidase (HRP)-conjugated secondary antibody and Amplex Red soluble fluorophore were purchased from Invitrogen (Carlsbad, CA, USA). The SpectraMax M5 microplate reader was purchased from Molecular Devices Corp. (Sunnyvale, CA, USA). BCA reagents were from Pierce Chemical Corp. (Rockford, IL, USA). All other fine chemicals were purchased from CalBiochem (Carlsbad, CA, USA), Pierce (Rockford, IL, USA) or Sigma (St. Louis, MO, USA).
Tong M., Gonzalez-Navarrete H., Kirchberg T., Gotama B., Yalcin E.B., Kay J, & de la Monte S.M. (2017). Ethanol-Induced White Matter Atrophy Is Associated with Impaired Expression of Aspartyl-Asparaginyl-β-Hydroxylase (ASPH) and Notch Signaling in an Experimental Rat Model. Journal of drug and alcohol research, 6, 236033.
+ Open protocol
+ Expand

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