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Camedia c 5060

Manufactured by Olympus
Sourced in Japan

The Camedia C-5060 is a digital camera designed for professional and advanced amateur use. It features a 5.0-megapixel CCD image sensor and a 3x optical zoom lens. The camera supports various file formats, including JPEG and TIFF, and offers manual controls for exposure, white balance, and other settings.

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12 protocols using camedia c 5060

1

Senescence Analysis via SA-β-gal Staining

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After repeated mimic-miRNA siRNA transfection (twice during 6 days) cells were washed in 1xPBS and fixed in 2% formaldehyde/0.2% glutaraldehyde for 15 minutes at room temperature. Cells treated with 100 nM Adriamycin were used as positive control. Cells were washed and incubated with fresh SA-β-gal staining solution containing 1mg/ml X-gal, 40 mM citric acid/sodium phosphate (pH 6.0), 5 mM potassium ferrocyanide, 5 mM potassium ferrocyanide, 150 mM NaCl and 2 mM MgCl2 for 16-18 h at 37 °C. Blue-stained senescent cells were counted using a light microscopy (Olympus IX71, Olympus America, PA, USA); images were captured using a digital camera (Olympus-Camedia C-5060).
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2

Assessment of Root Tissue Viability

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The viability of root tissue was assessed using the fluorescein diacetate/propidium iodide (FDA/PI) staining mixture method as described by Pan et al. (2001) (link). The tips of roots (about 2.5 cm in length) were gently incubated in a FDA/PI mixture (containing FDA 12.5 g mL-1 and PI 5.0 g mL-1) for 10 min and then washed with distilled water. Images were observed with a fluorescent microscope (Olympus BX41; Olympus Corporation, Tokyo, Japan) under blue light excitation (460–495 nm) and emission at 510 nm and analyzed using a photographic apparatus (Olympus CamediaC-5060; Olympus, Tokyo, Japan) and analySIS software (Soft Imaging Solutions GmbH, Münster, Germany).
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3

Histological Evaluation of Fibrosis and Inflammation

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Serial sections were stained with haematoxylin and eosin (HE) and Sirius red to evaluate the presence of inflammation, fibrosis and tumours12 (link). Three images per section were captured at 100 × magnification using an Olympus BX41 microscope (Olympus, Tokyo, Japan) equipped with a 10 × /0.40 numerical aperture objective lens and an Olympus Camedia C-5060 camera. The percentage of Sirius red staining, which indicated the presence of collagen fibres, was evaluated for the whole area of each image using the ImageJ software (NIH, Bethesda, MD, USA).
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4

Staining and Quantifying Osteoclasts

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The 24-well culture plates from the osteoclast cultures were processed for TRAP cell staining immediately. The method was modified from Boeyens et al. (2014) (link). Briefly, the cells were fixed with fixative buffer then washed with warm deionized water. A 500 μL of 0.125 mg/mL naphthol-AS-BI phosphate in acetate-tartrate buffer was added and incubated at 37 °C for 30 min. Then, pararosaniline dye in acetate-tartrate buffer was added and incubated for 15 min. Hematoxylin was used for counter staining. All TRAP-positive multinucleated cells containing more than 4 nuclei in each well were counted as osteoclasts under 10× objective lens of an inverted microscope (Olympus CK40). Images were taken with an Olympus camedia C-5060 camera.
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5

Documenting Pupal Pigmentation Differences

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To reveal the pigmentation differences between the SEW and SEM strains, we observed and documented the pigmentation time courses of the SEW and SEM pupae with a digital camera (Olympus Corp., Camedia C-5060, Japan) under white light. The pupae of both strains were observed and pictured immediately after pupation (zero time point), once per 15 min in the first two hours post pupation, once per 2 hours from 2 to 12 hours, once per 4 hours from 12 to 24 hours, and then once per day until eclosion.
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6

Histopathological Evaluation of Rabbit Eyes

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After seven days of treatment, the rabbits were euthanized. The whole right and left eyes were dissected from all groups and fixed in 10% neutral-buffered formalin for 48 h. Then, these eyes were sliced just behind the cornea to divide the ball into anterior and posterior parts. Selected samples represented the cornea, sclera, ciliary body and their processes, iris, retina, and choroid were fixed in 10% neutral buffered formalin for 24 h, processed, sectioned at 4–6 µm thickness, stained by Hematoxylin and Eosin (H and E), and finally, examined by light microscope (Olympus microscope, CX3I, Tokyo, Japan) and photographed using a digital camera (Olympus, Camedia C-5060, Tokyo, Japan). Tissues were scored from 0 (normal) to 4 (marked) signs of inflammation. Scores were combined to give a total inflammatory score of 20 using the following criteria: (i) edema or congestion in the cornea, iris, ciliary process, and choroid, (ii) inflammatory cell infiltration in the cornea, ciliary process, and retinal tissues, and (iii) neovascularization in the cornea [44 (link)].
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7

Particle Characterization Using Microscopy

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The particles were examined with the use of an Olympus BX41 light microscope (Olympus, Hamburg, Germany). Images were registered with a photographic camera (Olympus Camedia C-5060, Hamburg, Germany). Images obtained at 40× magnification were binarized and segmented (Fiji image processing). ImageJ software was used to measure particle size parameters.12 Feret diameter and circularity were selected as the most representative shape descriptors. The results were analyzed in GrapPad Prism 5.0 (GrapPad Software, San Diego, CA). The surface layer of the samples was observed with a TESCAN VEGA3 LMU scanning electron microscope (SEM) (Tescan, Brno, Czech Republic). A graphite double-sided adhesive tape was covered with the sample; afterwards, the sample was dehydrated and sputter-coated with gold (Emitech K550X, Quorum Technologies, UK). The analyses were performed at acceleration voltage of 30 kV.
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8

Histopathological Analysis of Avian Tissues

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Fresh specimens from liver, bursae and lung of chicken from 3 birds at 3, 5, 7, 9, and 35 day post infection were collected and fixed in 10% neutral-buffered formalin. The tissues were dehydrated in a graded alcohol series, cleared with methyl benzoate, embedded in paraffin wax, sectioned at 4-μm thickness and stained with haematoxylin and eosin for histopathological examination by light microscopy (Olympus CX31, Japan) and photographed using digital camera (Olympus, Camedia C-5060, Japan) [30 (link)].
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9

Histopathological Analysis of Cecal Lesions

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Fresh cecal specimens from five birds per experimental group were formalin-fixed and processed for histopathological analysis. Hematoxylin and eosin were applied to 5 µm-thick paraffin sections and stained with hematoxylin and eosin (H&E). The microscopic examination was conducted by light microscopy (Olympus CX31, Tokyo, Japan) and photographed with a digital camera (Olympus, Camedia C-5060, Tokyo, Japan) in the Photomicrograph Lab of the Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, Assiut University [29 (link),30 ]. All detected microscopic lesions were scored according to their severity, as described elsewhere [11 (link)] as follows: 0: normal, 1: minimal severity, 2: mild severity, 3: moderate, 4: marked, and 5: severe.
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10

Histopathological Examination of Tissues

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5.6.1. Histopathological examination: Tissue specimens from bursa, thymus, spleen, lung, liver, intestine and heart of all experimental groups were collected at 21 of age and fixed in 10% neutral buffered formalin. The tissues were prepared for routine histopathological examination (Bancroft et al., 2013) and examined using the light microscope (Olympus CX31, Japan) and photographed using a digital camera (Olympus, Camedia C-5060, Japan).
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