IFS procedures were previously used in our laboratory [14 (link)], and they were performed during the light phase (14:00–18:00h). The shock apparatus consisted of a shock generator (Coulbourn Instruments, Lehigh Valley, PA, USA) and a shock chamber (26×30×30 cm, model H10-11R-TC) with grid flooring inside a sound attenuated cubicle (80×50×50 cm). Foot-shocks were delivered through the grids by a computerized shock system (Habitest system; Graphic State 3.0 software). Each rat was placed individually in the chamber, left undisturbed for 2 min before receiving a total of 10 foot-shocks of 1mA intensity, each of 5s duration, with an inter-shock interval from 24s to 244s (mean 90s). The apparatus was thoroughly cleaned with a 20% ethanol solution between animals.
H10 11rtc
Manufactured by Harvard Apparatus
Sourced in United States
The H10-11RTC is a laboratory instrument designed for precise temperature control. It features a temperature range of 4°C to 70°C and can be used to maintain a steady temperature in various experimental setups.
Automatically generated - may contain errors
Lab products found in correlation
Freezeframe software, by Actimetrics (4 mentions)
Sound attenuating cubicle, by Med Associates (1 mentions)
Freezeframe, by Actimetrics (1 mentions)
Env 007, by Med Associates (1 mentions)
Phm 100, by Med Associates (1 mentions)
Med pc 4 software, by Med Associates (1 mentions)
Vah95ab, by Instech (1 mentions)
10 protocols using h10 11rtc
1
Foot-Shock Conditioning Protocol
2
Contextual Fear Conditioning in Mice
3
Contextual Fear Conditioning in Mice
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On day 1 of the behavioral experiment, mice were subjected to contextual fear conditioning, which consisted of three training sessions each separated by 3 h. At the start of each fear conditioning session, the mouse was transferred to a plexi-glass box with a grid floor (Coulbourn Instruments, H10-11RTC, 120 wide × 100 deep × 120 high) contained within an isolation chamber. Foot shocks (2 s each, 0.70 mA) were delivered at 198, 278, 358, and 438 s, with a total session time of 500 s. Mice were returned to their home cage in between each session. On day 4, mice were subjected to a 500-s retrieval test. Mice were placed in the context used for fear conditioning but did not receive foot shocks during the testing session. The sessions were recorded with an above digital camera and freezing behavior was quantified using Actimetrics FreezeFrame software. The bout length of freezing was set to 1 s, and the threshold for freezing was determined by an experimenter blinded to group. Mice in the home cage group remained in their cage throughout the duration of the experiment.
4
Contextual Fear Conditioning Chamber Setup
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The CFC chamber (H10-11R-TC, Coulbourn Instruments, Whitehall, PA, USA, 30.48 × 25.4 × 30.48 cm) has transparent acrylic back and front walls, and steel side panels. The grid floor has electrifiable stainless steel-bars (0.5 cm in diameter, separated by 1.0 cm) connected to a shock generator (H13-15, Coulbourn). A digital camera (SenTech, Carrollton, TX, USA) was located on the ceiling of the chamber, and a red light and a white light were located on the opposite sidewalls. Both the shock generator and the lights were connected to a USB interface (ACT-710, Actimetrics, Wilmette, IL, USA), which, along with the camera, were controlled by the FreezeFrame (Actimetrics) software installed in a computer running Microsoft Windows XP. To avoid auditory disturbances, a speaker emitting white noise (60 dB) was present during all behavioral procedures. The chamber was located inside a sound-attenuating cubicle (Med Associates Inc., USA) located in a sound-attenuated room.
5
Threat Conditioning and Passive Avoidance
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Threat conditioning occurred in standard conditioning boxes (H10-11R-TC: Coulbourn Instruments, Whitehall PA). USAA training and PIT testing occurred in two-way shuttleboxes (H10-11R-SC: Coulbourn Instruments). Conditioning boxes and shuttleboxes were housed in sound-attenuating chambers (model H10-24A). All boxes were equipped with house lights, infrared indicator lights, video cameras and 8 Ohm speakers to deliver the tone CS (generated by a programmable tone generator: model A12-33). The scrambled footshock US was delivered through stainless steel grid floors (model H10-11R-TC-SF). Shuttleboxes were also equipped with infrared beams to automatically detect movement between the two chamber sides.
6
Contextual Fear Conditioning in Mice
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Male and female, 2–3 month old mice were singly housed for at least 3 days prior to behavioral testing. On day 0, mice were submitted to one contextual FC trial. FC trials were performed in a specialized chamber (Coulbourn Instruments; H10-11RTC, 120W × 100D × 120H) and consisted of 500 s with 2 s long, 0.7 mA foot shocks administered at 198, 278, 358, and 438 s.
7
Operant Conditioning Chambers for Rat Experiments
8
Contextual Modulation of Pavlovian Conditioning
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Pavlovian conditioning took place in context A, a set of standard training chambers manufactured by Coulbourn Instruments (26 × 28 × 20 cm, length × width × height; model no H10-11R- TC) with stainless steel grid floors to deliver the footshock US and an 8-Ω speaker for the 30-s 5-kHz tone CS presentations. Avoidance training and PIT testing took place in context B, which was a two-way shuttle chamber (50.8 × 25.4 × 30.5 cm, model no H10- 11R-SC) also manufactured by Coulbourn. Stainless steel grid floors similar to those in context A presented the footshock US and had an 8-Ω speaker on each side of the chamber to present the tone CS. All chambers were housed in light and sound attenuating shells. Follow-up tests for CS-elicited freezing were conducted in context C. Context C was another set of standard training chambers also manufactured by Coulbourn in a different room and made different from context A by insertion of striped patterns on the plastic walls and peppermint scent (Dr. Bronner’s Magic Soaps) in the waste pan. Additionally, the floor was made from thin mesh-wiring.
9
Contextual Fear Conditioning in Mice
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Male and female, 2-3 month old heterozygous Camk2a-tTA/tetO-EGFP-L10a mice on a C57BL/6J background were singly housed and either left in their cage until tissue dissection or submitted to a single contextual fear conditioning trial. Fear conditioning trials were performed in a specialized chamber (Coulbourn Instruments; H10-11RTC, 120W × 100D × 120H) and consisted of 500 seconds with 2 second, 0.7 mA foot shocks administered at 198, 278, 358, and 438 seconds. Freezing behavior was measured using a digital camera connected to a computer with Actimetrics FreezeFrame software. Before shock freezing scores were obtained by averaging freezing during minute 2 and 3 of the trial. After shock freezing scores were obtained by averaging freezing during the final 40 seconds of the trial.
10
Contextual Fear Conditioning in Mice
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At 14 days after stereotaxic surgery, mice underwent a single contextual fear conditioning trial in a specialized chamber (H10-11RTC, 120W × 100D × 120H; Coulbourn Instruments). The trial lasted 500 s with 2 s, 0.7 mA foot shocks administered at 198, 278, 358 and 438 s. Freezing behavior was measured using Actimetrics FreezeFrame software during two intervals before the first shock (0–60 s, 60–180 s), and during intervals starting 20 s after each of the four shocks (220–260 s, 300–340 s, 380–420 s, and 460–500 s).
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