Antibody against β catenin

Antibodies against Flag-M2, hnRNPK/J and β-actin were purchased from Sigma-Aldrich. Antibody against GAPDH was obtained from ENOGENE. Antibody against β-catenin was acquired from BD Biosciences. Antibody against Twist was purchased from GeneTex. Antibody against Aurora-A was obtained from Abcam as was anti-mono/dimethyl arginine antibody. Finally, a specific antibody was custom ordered from GeneTex that allowed detection of hnRNPK S379 phosphorylation.

For isolation of total lysates, cells were pelleted after 1× PBS wash and lysed in RIPA lysis buffer (Thermo #89900) supplied with protease inhibitor cocktail (Sigma #11836170001). Lysates were collected in new pre‐chilled tubes and protein concentration was measured using Bradford reagent (Bio‐Rad #500‐025) and BSA standards (Bio‐Rad #500‐027). Equal amount of protein was mixed with 4× gel loading dye (Thermo #NP007) and analyzed by pre‐casted gel (Thermo #NP0036) and Western blotting. Final western blots were detected on a GE Healthcare Life Sciences, Amersham Imager 600, followed by analyzing band intensities with ImageJ (U.S. National Institutes of Health, Bethesda, MD, USA).
Antibodies used for western blotting were as follows: anti‐LRP6 (#S2560) primary antibody was purchased from Cell Signaling Technology, antibody against anti‐GAPDH (#GTX627408) was from GeneTex, anti‐Vinculin (#sc‐736914) primary antibody was from Santa Cruz, antibody against β‐catenin (#610153) was from BD Biosciences, anti‐MCL‐1 (#ab32087), anti‐BCL‐2 (#ab692) primary antibodies were from Abcam.