Silica gel 70 f254 tlc plates

The starting materials, reagents, and solvents were purchased and used without further purification. We used Silica gel 70 F254 TLC plates (Wako) for analytical Thin-layer chromatography (TLC), while Silica gel 60 N (spherical, neutral, Kanto Chemical) were for column chromatography. For preparative flash chromatography, used were an automated system (Smart Flash EPCLC AI-580S, Yamazen Corp., Japan) equipped with universal columns of silica gel. Melting points and IR spectra were determined with a MP-500P (Yanaco) and a Nicolet 6700 spectrometer with anATR attachment, respectively. ¹H and ¹³C NMR spectra were determined on a JEOL ECA-500 instrument (500 MHz for ¹H and 126 MHz for ¹³C). Mass spectra were obtained with a high-resolution electro-spray ionization mass spectrometer, JMS-T100LC (JEOL). UV/visible absorption spectra were determined with a spectrophotometer, Cary 60 (Agilent Technologies) (scan speed 600 nm/min; data interval 1 nm). BL and chemiluminescence spectra were measured with a precision spectrophotometer, AB-1850 (ATTO) (data interval: 1 nm). BL intensities were monitored using luminometers, AB-2270 (ATTO) and GL-201A (Microtec Co.). BL imaging was performed with a multifunctional in vivo imaging system (IVIS Spectrum, PerkinElmer).

The starting materials, reagents, and solvents were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan), FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan), Kanto Chemical Co., Inc. (Tokyo, Japan) and Sigma-Aldrich (Tokyo, Japan), and used without further purification. Silica gel 70 F254 TLC plates (FUJIFILM Wako, Osaka, Japan) were used for analytical Tin-layer chromatography (TLC), while Silica gel 60 N (spherical, neutral, Kanto Chemical (Tokyo, Japan)) was for column chromatography. For preparative flash chromatography, used was an automated system (Smart Flash EPCLC AI-580S, Yamazen Corp., Osaka, Japan) equipped with universal columns of silica gel. ¹H and ¹³C NMR spectra were determined with a JEOL ECA-500 instrument (500 MHz for 1 H and 126 MHz for ¹³C) (JEOL, Tokyo, Japan). Mass spectra were obtained with a high-resolution electrospray ionization mass spectrum (JMS-T100LC) (JEOL, Tokyo, Japan) and Matrix-assisted laser desorption/ionization mass spectrum (JMS-S3000 SpiralTOF^TM-plus 2.0) (JEOL, Tokyo, Japan).

The starting materials, reagents, and solvents were purchased from Tokyo Chemical Industry Co., Ltd., FUJIFILM Wako Pure Chemical Corporation, Kanto Chemical Co., Inc., and Sigma-Aldrich, and were used without further purification. Silica gel 70 F254 TLC plates (Wako) were used for analytical thin-layer chromatography (TLC), whereas Silica gel 60 N (spherical, neutral; Kanto Chemical) was applied in column chromatography. For preparative flash chromatography, an automated system (Smart Flash EPCLC AI-580S; Yamazen Corp., Japan) equipped with universal columns of silica gel was used. The ¹H and ¹³C NMR spectra were determined using a JEOL ECA-500 instrument (500 MHz for 1 H and 126 MHz for 13C). Mass spectra were obtained with a high-resolution electrospray ionization mass spectrometer (JMS-T100LC; JEOL) and a matrix-assisted laser desorption/ionization mass spectrometer (JMS-S3000 SpiralTOF^TM-plus 2.0; JEOL).