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Enzyme linked immunosorbent instrument

Manufactured by Thermo Fisher Scientific
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The enzyme-linked immunosorbent instrument is a laboratory equipment used to perform enzyme-linked immunosorbent assays (ELISA). This instrument is designed to detect and quantify specific proteins, antibodies, or other analytes in a sample by employing enzyme-labeled reagents and a colorimetric or fluorometric detection method.

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Lab products found in correlation

96 well plate, by Corning (2 mentions) Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions) Anti il 10, by Abcam (1 mentions) Rhil 10, by R&D Systems (1 mentions) Cell counting kit 8 (cck8), by Dojindo Laboratories (1 mentions)

2 protocols using enzyme linked immunosorbent instrument

1

Cell Proliferation Assay with CCK8

Check if the same lab product or an alternative is used in the 5 most similar protocols
The cell proliferation ability was determined using the Cell Counting Kit-8 (CCK8, Dojindo, Japan). Briefly, 3000 cells were seeded into 96-well plates (Corning, USA). At the indicated times, each well medium was replaced with an equal volume of fresh medium containing 10% CCK-8 reagent. After cells were incubated at 37 °C for 2.5 h, absorbance was measured using an Enzyme-linked immunosorbent instrument (Thermo-Fisher, USA) at 450 nm. Recombinant human interleukin 10 (rhIL-10, R&D Systems, USA) and its neutralizing antibody (Anti-IL-10, Abcam, UK) were used in this study.
Yuan H., Lin Z., Liu Y., Jiang Y., Liu K., Tu M., Yao N., Qu C, & Hong J. (2020). Intrahepatic cholangiocarcinoma induced M2-polarized tumor-associated macrophages facilitate tumor growth and invasiveness. Cancer Cell International, 20, 586.
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2

Cell Proliferation Assessment with CCK-8

Check if the same lab product or an alternative is used in the 5 most similar protocols
The cell proliferation ability was determined using the Cell Counting Kit-8 (Dojindo, Kumamoto, Japan). Briefly, 3000 cells were seeded into 96-well plates (Corning). Each well medium was replaced with an equal volume of fresh medium containing 10% CCK-8 reagent at the indicated times. After incubating cells at 37 °C for 2 h, absorbance was measured using an enzyme-linked immunosorbent instrument (Thermo Fisher Scientific, Waltham, MA) at 450 nm.
Gao C.Q., Chu Z.Z., Zhang D., Xiao Y., Zhou X.Y., Wu J.R., Yuan H., Jiang Y.C., Chen D., Zhang J.C., Yao N., Chen K.Y, & Hong J. (2023). Serine/threonine kinase TBK1 promotes cholangiocarcinoma progression via direct regulation of β-catenin. Oncogene, 42(18), 1492-1507.
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