A Zeiss Sigma FEG VP SEM with EDS and an FEI Tecnai F20 HRTEM equipped with STEM and EDS capabilities operated at an accelerating voltage of 200 kV were used for the imaging and chemical analysis of the samples. EELS data acquisition was carried out using a JEOL F200 STEM microscope equipped with a Gatan GIF Quantum IS that features a direct detection K2 camera which is capable of acquiring EELS data in electron counting mode, as well as low-dose energy filtered and TEM images at a frame rate up to 1,600 images in in situ mode (Hart et al., 2017 (link)). For SEM analysis, samples were loaded onto a standard aluminum stub with a double-sided carbon and single-sided copper tape. For TEM analysis, samples were drop-cast onto 300 mesh copper grids with lacey carbon coatings (Ted Pella Inc., # 01895, Redding, California).
Tecnai f20 hrtem
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Tecnai F20 HRTEM is a high-resolution transmission electron microscope (HRTEM) designed and manufactured by Thermo Fisher Scientific. It provides high-resolution imaging and analysis capabilities for a wide range of materials and applications. The Tecnai F20 HRTEM is capable of achieving a resolution of up to 0.19 nanometers, allowing for the detailed examination of atomic-scale structures.
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Lab products found in correlation
Spectrum two, by PerkinElmer (1 mentions)
Zetasizer nano zs90, by Malvern Panalytical (1 mentions)
Polarstar omega microtitre plate reader, by BMG Labtech (1 mentions)
Cm100 transmission electron microscope, by Philips (1 mentions)
Tecnai f20, by Thermo Fisher Scientific (1 mentions)
Cary 100 uv vis, by Agilent Technologies (1 mentions)
Spectrum two ftir, by PerkinElmer (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Nicotinamide, by Merck Group (1 mentions)
Thiamine hydrochloride, by Merck Group (1 mentions)
Pyridoxine hydrochloride, by Merck Group (1 mentions)
Calcium d pantothenate, by Merck Group (1 mentions)
6 protocols using tecnai f20 hrtem
1
Multi-Modal Microscopy Analysis Methodology
2
Structural Characterization of Colloidal Samples
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HRTEM image was used to obtain detailed structural and morphological information about the samples and was carried out using a FEI Tecnai F20 HRTEM. The samples for HRTEM images were prepared by depositing a drop of a diluted colloidal solution on a carbon grid and allowing the liquid to dry in air at room temperature.
3
Characterization of Silver Nanoparticles
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HRTEM analysis was performed by the addition of a drop of each AgNP solution on carbon-coated copper grids, then left to dry under ambient conditions. The shape and size of AgNPs were analyzed using TecnaiF20 HRTEM (FEI Company, Hillsboro, OR, USA) with an accelerating voltage of 300 kV at the Electron Microscope Unit (University of Cape Town, South Africa). In addition, the core size distribution of the AgNPs was calculated using ImageJ software (National Institutes of Health, Bethesda, MD, USA).
4
Biogenic Gold Nanoparticle Synthesis and Characterization
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The biogenic AuNPs were washed thrice in double distilled water and centrifuged at 14,000 rpm for 15 min, the AuNPs were resuspended in equal volume of double distilled water. The SPR of the AuNPs was determined using a POLARstar Omega microtitre plate reader (BMG Labtech, Offenburg, Germany) at a wavelength range of 400–800 nm. The hydrodynamic diameter, PDI and zeta potential of the AuNPs were determined using a Nano-ZS90 Zetasizer instrument (Malvern Panalytical Ltd, Enigma Business Park,, UK). A Perkin Elmer Spectrum Two Fourier transform infrared (FTIR) spectrophotometer (Waltham, MA, USA) was used to determine the functional groups in HBE, MGF, and their respective AuNPs. A high-resolution transmission electron microscope (TecnaiF20 HR-TEM, FEI Company, Hillsboro, OR, USA) linked with energy dispersive X-ray spectroscopy (EDX) was used to determine the morphology and crystalline nature of the AuNPs.
5
Nanoparticle Morphology and Crystallography
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The microstructure and morphology were investigated using a Philips CM100 Transmission Electron Microscope (TEM) with an acceleration voltage U = 80 kV. The quantitative analysis of the nanoparticle sizes and shapes was performed manually by measuring the nanoparticle sizes in the TEM images using the program Scion. The amount of nanoparticles for each different shape was counted manually and normalized by the total amount of nanoparticles used for the statistical evaluation. A statistically sufficient amount of nanoparticles (>> 500) was used for the quantitative analysis. A FEI TECNAI F20 HRTEM with an acceleration voltage U = 200 kV was used for a detailed structural analysis. Electron diffraction measurements were also conducted with the FEI TECNAI F20. Since the distances and angles between the reflexes are material and zone axis specific, indexing the diffraction patterns yields information on the orientation and crystal faces of the different particles.
6
Synthesis and Characterization of Nicotinamide-Derived Carbon Quantum Dots
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All standards or chemical reagents used were of pharmaceutical and analytical grades. Nicotinamide, pyridoxine hydrochloride (vitamin B6), thiamine hydrochloride (vitamin B1), calcium D-pantothenate (vitamin B5), and sodium hydroxide were purchased from Merck (Darmstadt, Germany) and cyanocobalamin (vitamin B12) and citric acid (CA) were supplied from Sigma-Aldrich.
Ultrapure water was puri ed with a Millipore (0.05 µS cm - 1 ) instrument. All UV-Vis absorption spectra were measured by a UV-Vis spectrophotometer (Cary 100 UV-Vis, Agilent Technologies) from 200 to 800 nm. Fluorescence spectra of VB12 standard and sample solutions were recorded from 370 to 700 nm by a uorescence spectrophotometer (LS-55, Perkin Elmer) equipped with a Xenon lamp source and a 1.0 cm quartz cell. Surface morphology microscopy of NA-CQDs were performed using a SEM (ZEISS SIGMA VP FE-SEM with Oxford EDS and Mapping) and high-resolution TEM (FEI Tecnai F20 HR-TEM) microscope.
The FT-IR spectrum of NA-CQDs were acquired using a Fourier transform infrared spectrometer (FT-IR Spectrum two, Perkin Elmer) from 400 to 4000 cm - 1 .
Ultrapure water was puri ed with a Millipore (0.05 µS cm - 1 ) instrument. All UV-Vis absorption spectra were measured by a UV-Vis spectrophotometer (Cary 100 UV-Vis, Agilent Technologies) from 200 to 800 nm. Fluorescence spectra of VB12 standard and sample solutions were recorded from 370 to 700 nm by a uorescence spectrophotometer (LS-55, Perkin Elmer) equipped with a Xenon lamp source and a 1.0 cm quartz cell. Surface morphology microscopy of NA-CQDs were performed using a SEM (ZEISS SIGMA VP FE-SEM with Oxford EDS and Mapping) and high-resolution TEM (FEI Tecnai F20 HR-TEM) microscope.
The FT-IR spectrum of NA-CQDs were acquired using a Fourier transform infrared spectrometer (FT-IR Spectrum two, Perkin Elmer) from 400 to 4000 cm - 1 .
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