U251 and U87 human glioma cells were transfected at 80% confluence with SCAP siRNA (cat. no. sc-36462; Santa Cruz Biotechnology, Inc.), hypoxia-inducible factor 1 (HIF-1) siRNA (cat. no. sc-35561; Santa Cruz Biotechnology, Inc.), SREBP-1 siRNA (cat. no. sc-36557; Santa Cruz Biotechnology, Inc.) and control siRNA (cat. no. sc-37007; Santa Cruz Biotechnology, Inc.) using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions. Briefly, prior to treatment, Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.)/siRNA complexes were prepared in Opti-MEM medium, at the ratio of 1 μl Lipofectamine 2000 per 20 pmole siRNA. Cells then were then treated at 37°C for 48 h before being replaced with complete medium for the desired duration. The transfection efficiency of the cells was verified by western blotting.
Hif 1 sirna
Manufactured by Santa Cruz Biotechnology
HIF-1) siRNA is a synthetic small interfering RNA (siRNA) designed to target the hypoxia-inducible factor 1-alpha (HIF-1) gene. The core function of this product is to facilitate the downregulation of HIF-1 expression in cells, which can be used as a tool for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Srebp1 sirna, by Santa Cruz Biotechnology (1 mentions)
Control sirna, by Santa Cruz Biotechnology (1 mentions)
Ar c155858, by Bio-Techne (1 mentions)
U 14c glucose, by PerkinElmer (1 mentions)
Mito tempo, by Santa Cruz Biotechnology (1 mentions)
Anti hif 1α, by BD (1 mentions)
N biotinoyl n iodoacetyl ethylenediamine biam, by Thermo Fisher Scientific (1 mentions)
2 7 dichlorofluorescein diacetate h2dcfda, by Thermo Fisher Scientific (1 mentions)
U 14c lactate, by PerkinElmer (1 mentions)
Metformin, by Merck Group (1 mentions)
2 protocols using hif 1 sirna
1
Silencing Key Regulators in Glioma Cell Lines
2
Metabolic Regulation in Cancer Cells
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Unless specified, all reagents were obtained from Sigma and all the antibodies were from Santa Cruz Biotechnology, except for anti-HIF-1α (Becton Dickinson), anti-PKM2 and anti-Src-Tyr416 (Cell Signaling Technology) and anti phospho-Tyr (clone 4G10) (Millipore). HRP-conjugated streptavidin was from Pierce. HIF-1-siRNA and DEC1-siRNA were from Santa Cruz Biotechnology. N-(biotinoyl)-N-(iodoacetyl)ethylenediamine (BIAM) and 2′-7′-dichlorofluoresceindiacetate (H2-DCF-DA) were from Molecular Probe. [U-14C] lactate and [U-14C] glucose were from Perkin Elmer. All the kits used to perform miRNA extraction and quantitative reverse transcriptase PCR were from Qiagen. Lipofectamine 2000 was from Invitrogen. Metformin was obtained by Sigma. The chemical synthesis of the PKM2 activator DASA-58 was kindly performed by Dr. Richichi of the Department of Chemistry (University of Florence). The mitochondrial antioxidant MitoTEMPO was from Santa Cruz Biotechnology. The MCT1 inhibitor, AR-C155858, was from Tocris Bioscience.
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