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3 protocols using orthophosphoric acid hplc grade

1

In vitro Cytotoxicity of Doxorubicin-Chitosan Nanoparticles

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Doxorubicin hydrochloride, chitosan (Mv 110,000–150,000), bovine serum albumin (fraction V), fetal bovine serum, acetonitrile (HPLC grade) and orthophosphoric acid (HPLC grade) were purchased from Sigma Chemical Co. (Merck, Darmstadt, Germany). For the in vitro cytotoxicity studies, keratinocytes HaCaT (CLS Cell Lines Service, 300493, Eppelheim, Germany) and epidermoid squamous skin carcinoma cells A-431 (CLS Cell Lines Service, 300112, Eppelheim, Germany) were used.
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2

Chitosan-based Mucoadhesive Formulation

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Chitosan (MW 100,000–300,000), propranolol hydrochloride, agar powder, porcine stomach mucin, acetonitrile, methanol and orthophosphoric acid (HPLC grade) were purchased from Sigma-Aldrich (Germany). Potassium dihydrogen phosphate, sodium hydroxide, potassium thiocyanate, potassium chloride and calcium chloride were purchased from May & Baker (Dagenham, England). Poly vinyl alcohol (25% polymerization degree 5.00–2000), Carbopol 971P (CP971P), PEG 400, propylene glycol, were purchased from Fisher Chemical (Leicestershire UK). Metoprolol (99.87%); The internal standard was supplied by Sedico Company, Egypt.
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3

Favipiravir Quantification Protocol

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Favipiravir standard (FAV) was provided by BDR Pharmaceuticals International Pvt. Ltd. (Mumbai, India) with purity of 99.98% (according to supplier certificate of analysis). Avigan® tablets, produced by Fujifilm Toyama Chemical Co., Ltd, Japan, Batch No. FG1881, labelled to contain 200 mg FAV per tablet, was purchased from the local market.
Methanol, acetonitrile, monobasic potassium phosphate, heptane sulphonic acid sodium salt, orthophosphoric acid (HPLC grade) were purchased from Sigma-Aldrich (Milwaukee, USA). Hydrochloric acid (36%), sodium hydroxide, hydrogen peroxide (30%), analytical grade, were obtained from Merck (Mumbai, India). Ultra-pure deionized water was obtained from MilliQ UF-Plus water purification system (Millipore, Bedford, MA, USA).
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