Murine cytokine specific elisa kits

According to a previously described method (23 (link)), BALF was collected by flushing 1 ml ice-cold PBS back and forth three times through a tracheal cannula, and then centrifuged at 1,000 × g at 4°C for 10 min. The protein concentrations of IL-6 (cat. no. BMS603-2), IL-8 (cat. no. EMCXCL15), TNF-α (cat. no. BMS607-3), IL-17 (cat. no. BMS6001), IL-18 (cat. no. BMS618-3) and IL-1β (cat. no. EM2IL1B2) in the supernatant were measured using murine cytokine-specific ELISA kits (Invitrogen Life Technologies; Thermo Fisher Scientific, Inc.) in strict accordance with the manufacturer's protocol. The quantities of total protein (cat. no. ABIN996404), albumin (cat. no. ABIN2756308) and KGF (cat. no. ABIN2703018) in the BALF were measured as markers of epithelial permeability using ELISA kits (Antibodies-Online GmbH).

BALF was collected by flushing 1 ml ice-cold PBS back and forth three times through a tracheal cannula followed by centrifugation at 2,000 × g at 4°C for 10 min, as previously described (22 (link)). Protein concentrations of IL-4, IL-6, IL-13, IL-17, TNF-α and IFN-γ in the supernatant were measured using murine cytokine-specific ELISA kits (Invitrogen; Thermo Fisher Scientific, Inc.), according to the manufacturer's protocol. The concentration of IL-4, IL-6, IL-13, IL-17, TNF-α and IFN-γ in the BALF were measured as markers of inflammatory reaction using a microplate reader at 450 nm (Thermo Fisher Scientific, Inc.; catalog no. 51119000).