Mg132

BA (Meilunbio, Dalian, China; MB5971), Chloroquine (Sigma, St. Louis, MO, USA; C6628), E-64d (Sigma, E8640) and Pepstain A (Sigma, P4265) were dissolved in DMSO (Sigma, D2650). Acridine orange (Sigma, A6014) and 3-MA (Meilunbio, MB5063) were dissolved in PBS. MG132 (Meilunbio, MB5137), Etoposide (Meilunbio, MB1102-S) and CHX (Meilunbio, MB2208) were dissolved in DMSO. LC3 (MBL, Beijing, China; PM036) was bought for immunoblot or immunofluorescence analysis. Antibodies including AKT, phosphorylation of AKT (Ser473; 4060), MTOR, phosphorylation of MTOR (Ser2443; 5535), PARP (116 and 89 kd; 9542S), BAX (5023), phosphorylation of p53 (Ser15; 9286) and GAPDH (Santa Cruz Biotechnology(CA, USA) 5174) were purchased from CST (Beverly, MA, USA). Cleaved-PARP antibody was purchased from Sigma (SAB4500487). p53 (SC-126) and LAMP1 (SC-5570) were purchased from Santa Cruz Biotechnology (CA, USA) for immunoblot or immunofluorescence analyses.

The following antibodies were used: PARP16 (ARP33751) was obtained from Aviva (Aviva Systems Biology, USA); Aβ 6E10 (800304) was obtained from BioLegend (BioLegend, Inc. USA); sAPPβ (10321) and sAPPα (11088) were obtained from IBL (Immuno-Biological Laboratories Co., Ltd., Japan); p-IRE1α (PA1-16927) was obtained from ThermoFisher (Thermo Fisher Scientific Inc., USA); XBP-1 (ab37152), and Bcl-2 (ab196495) were obtained Abcam (Abcam); APP (2450), p-PERK (3179), p-eIF2α (3597), Bax (2772), Caspase 3 (9662), and p53 (2524) were purchased from CST (Cell Signaling Technology, Inc., USA); COX-2 (A3560), IL-1β (A1112) and IL-6 (A0286) were obtained from ABclonal (ABclonal Technology Co.,Ltd. China); iNOS (18985-1-AP), BIP (11587-1-AP), Calnexin (10427-2-AP), β-tubulin (10094-1-AP), β-Actin (66009-1-Ig), and GAPDH (60004-1-Ig) were purchased from Proteintech (Proteintech Group, Inc., USA). Actinomycin D was purchased from Linkchem (Shanghai LinkChem Technology Co., Ltd. China). MG132 was purchased from Meilunbio (Dalian, China). 3-Methyladenine (3-MA) and tauroursodeoxycholic acid (TUDCA) were obtained from MCE (MedChemExpress, USA). Aβ_1-42 (A9810) was purchased from Sigma-Aldrich (Sigma-Aldrich, USA).

Human prostate cancer cell line C4-2 was obtained from Dr. Jun Qin (Shanghai Institutes of Nutrition and Health Sciences, Chinese Academy of Sciences); PC-3, VCaP, and LNCaP cells were kindly provided by Cell Bank, Chinese Academy of Sciences; Mouse prostate cancer cell line Myc-CaP was obtained from Dr. Xuanming Yang (School of Life Sciences and Biotechnology, Shanghai Jiao Tong University); Human embryonic kidney 293 (HEK293T) cell line was obtained from Dr. Wenyi Wei (Harvard Medical School, MA). All the cell lines were cultured according to ATCC instructions. Cells were detected to be mycoplasma-free, and were characterized by Genesky Biopharma Technology using short tandem repeat (STR) markers. Reagents used in this study included MG132 (Meilun Biotech), Puromycin (Meilun Biotech), and Cycloheximide (CHX) (MedChemExpress).