Azd5363

Manufactured by Adooq

AZD5363 is a laboratory reagent that functions as an inhibitor of the protein kinase AKT. It is commonly used in scientific research applications to study the role of the AKT signaling pathway in various biological processes.

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Lab products found in correlation

Cm h2dcfda, by Thermo Fisher Scientific (1 mentions) Torin 1, by Adooq (1 mentions) Azd1208, by Adooq (1 mentions) Pim447, by Novartis (1 mentions) Anti β actin, by Merck Group (1 mentions) Anti irs 1, by Merck Group (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Anti 4e bp1, by Cell Signaling Technology (1 mentions) C myc, by Cell Signaling Technology (1 mentions) Anti pim1, by Cell Signaling Technology (1 mentions) Anti phopho s6 ser 235 236, by Cell Signaling Technology (1 mentions) Anti eif4b, by Cell Signaling Technology (1 mentions) Bkm 120, by Adooq (1 mentions) Anti s6, by Cell Signaling Technology (1 mentions)

2 protocols using azd5363

Cell Signaling Pathway Inhibition Assay

Cited 1 time

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PIM447 (17 (link)) was provided by Novartis Oncology. Buparlisib (BKM120) (18 (link)), AZD1208 (19 (link)), AZD5363 (20 (link)), and Torin1 (21 (link)) were purchased from AdooQ Bioscience. CM-H₂DCF-DA (22 (link)) was from Molecular Probes (Thermo Scientific). All other chemicals were purchased from Sigma-Aldrich.

Song J.H., Singh N., Luevano L.A., Padi S.K., Okumura K., Olive V., Black S.M., Warfel N.A., Goodrich D.W, & Kraft A.S. (2018). Mechanisms behind resistance to PI3K Inhibitor treatment induced by the PIM kinase. Molecular cancer therapeutics, 17(12), 2710-2721.

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Antibody and Kinase Inhibitor Validation

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The following antibodies were purchased from Cell Signaling Technology anti-Pim1 (Cat#2907), anti-S6(Cat#9202), anti-phopho-S6 (Ser 235/236 Cat#4856), anti-EDC3 (Cat #14495), anti-phospho-GSK3B, (Ser9, Cat#9336), anti-phopho-IRS1 S1101 (Cat#2385), anti-phospho-eIf4B S406 (Cat #5399), anti-eIF4B (Cat#3592), anti-AKT (Cat#9272), and anti-phopho-AKT S473 (Cat #4058), anti- phosphor 4E-BP1 (T37/46) (Cat#2855), anti-4E-BP1 (Cat#9644), c-Myc (Cat# 9402). The anti-phospho-EDC3 (S161 Cat#600-401-J38) was purchased from Rockland Antibodies while the HRP conjugated anti-β-actin (Cat#A3854) was from Sigma. Anti-IRS1 (Cat #06-248) was obtained from Merck Millipore. Antibodies bought from Santa Cruz Biotechnology included anti-EDC3 (Cat# sc-365024) and anti-GAPDH HRP (Cat. #0411). The kinase inhibitors BKM 120 (Cat# A11016) and AZD5363 (Cat#A11759) were purchased from Adooq Bioscience. VBT5445 was purchased from Chembridge.

Gnawali G.R., Okumura K., Perez K., Gallagher R., Wulfkuhle J., Petricoin E.F., Padi S.K., Bearss J., He Z., Wang W, & Kraft A.S. (2022). Synthesis of 2-Oxoquinoline Derivatives as Dual Pim and mTORC Protein Kinase Inhibitors. Medicinal chemistry research : an international journal for rapid communications on design and mechanisms of action of biologically active agents, 31(7), 1154-1175.

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