For this assay, 5 × 103 cells per well were plated in 96-well plates. Five days after seeding, the cell media was gently aspirated without touching adherent cellular layer and fixed with 50% trichloroacetic acid solution (Sigma-Aldrich, St. Quentin Fallavier, France) for 1 h at 4 °C. Following three washing steps with ddH2O, cells were stained with a solution of 0.4% sulforhodamine B (Sigma-Aldrich, St. Quentin Fallavier, France) in 1% acetic acid. Excess dye was removed by three washes with 1% acetic acid, and protein-bound dye was then dissolved in 10 mM Tris solution (pH 10.5). Absorbance was measured at 510 nm with a TriStar2 Multimodal Reader LB942 (Berthold Technologies). Data were plotted by using the SuperPlotsOfData webapp; the plots represent three independent experiments.
Tristar2 multimodal reader lb942
Manufactured by Berthold Technologies
The TriStar2 Multimodal Reader LB942 is a laboratory equipment designed to perform various analytical measurements. It can detect and quantify a range of analytes using different detection modes. The device's core function is to provide accurate and reliable data for scientific research and analysis purposes.
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2 protocols using tristar2 multimodal reader lb942
1
Sulforhodamine B Cell Viability Assay
2
Sulforhodamine B Cell Viability Assay
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Cells were plated into 96‐well plates (103 cells/well) and left overnight at standard growth conditions. At each desired time point, cells were fixed with 50% Trichloroacetic acid at 4°C for 1 h. After removing fixative solution, cells were stained with 0.4% Sulforhodamine B (Santa Cruz) in 1% acetic acid. The dye taken up by cells was then dissolved in 10 mM Tris‐base, pH 10.5. The absorbance was measured at 560 nm with TriStar2 Multimodal Reader LB942 (Berthold Technologies).
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