3 5 7 3h mtx 20 ci mmol

[3′,5′,7-³H]Mtx (20 Ci/mmol) was purchased from Moravek Biochemicals. Unlabelled Mtx was provided by the Drug Development Branch, National Cancer Institute, National Institutes of Health. Synthetic oligonucleotides were obtained from Invitrogen. Tissue culture reagents and supplies were purchased from assorted vendors with the exception of FBS, which was purchased from Hyclone Technologies. The cross-linking reagent 1,6-hexanediyl bismethanethiosulfonate (MTS-6-MTS) was purchased from Toronto Research Chemicals.

AGF94 [(S)-2-((5-[3-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)-propyl]-thio-phene-2-car-bonyl)-amino)-pentanedioic acid] was synthesized, as previously described [18 (link)]. Leucovorin [(6R,S) 5-formyl tetrahydrofolate] was obtained from the Drug Development Branch, National Cancer Institute, Bethesda, MD. [3′,5′,7-³H]MTX (20 Ci/mmol) was purchased from Moravek Biochemicals (Brea, CA). 5-Aza-2′-deoxycytidine (5-Aza) was purchased from Sigma–Aldrich (St. Louis, MO). Restriction and modifying enzymes were purchased from New England Biolabs (Ipswich, MA). The mammalian expression vector pcDNA3.1/myc-His(–)A was purchased from Invitrogen (Carlsbad, CA). Reporter gene vectors pGL3-Basic and pGL4.74[hRluc/TK] were purchased from Promega (Madison, WI). Other chemicals were obtained from commercial sources in the highest available purities.

[3′,5′,7-³H]MTX (20 Ci/mmol) was purchased from Moravek Biochemicals (Brea, CA). Unlabeled MTX was obtained from the Drug Development Branch, NCI, National Institutes of Health (Bethesda, MD). Folic acid, leucovorin [(6R,S)5-formyltetrahydrofolate], and fetal bovine serum (FBS) were purchased from Sigma Chemical Co. (St. Louis, MO). Other tissue culture supplies were purchased from various vendors.