To avoid diurnal variability [3 (link)], blood samples were taken at the same time for all volunteers, between 8–10 a.m. They were obtained with venipuncture into a vacutainer containing clot activator. Following inversion and clotting, the whole blood was centrifuged (LMC-3000 version V.5AD, Biosan, Riga, Latvia) for ten minutes at 3000 rpm. After that, serum was transferred into eppendorf tubes and stored frozen at −80 °C until further analysis. Within 1 to 15 days after testing, the serum samples were delivered to the clinical laboratory of the Spanish National Centre of Sport Medicine (Madrid, Spain) to determine sex hormones in order to verify hormonal profiles. Total E2, progesterone, FSH and LH were measured via ADVIA Centaur ® solid-phase competitive chemiluminescent enzymatic immunoassay (IMMULITE 1000 system; Siemens Healthineers AG, Munich, Germany). Inter- and intra-assay coefficients of variation (CV) reported by the laboratory for each variable were, respectively: 11.9% and 8.5% at 93.3 pg/mL and 6.8% and 4.7% at 166 pg/mL for E2; 23.1% and 11.8% at 0.7 ng/mL and 5.2% and 2.5% at 9.48 ng/mL for progesterone, 5.3% and 1.8% at 1.2 mIU/mL for FSH and 5.2% and 1.8% at 0.54 mIU/mL for LH.
Lmc 3000 version v 5ad
Manufactured by Biosan
Sourced in Latvia
The LMC-3000 version V.5AD is a laboratory centrifuge. It is designed to separate different components of a liquid sample based on their density and size.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using lmc 3000 version v 5ad
1
Diurnal Variation in Sex Hormone Profiles
2
Comprehensive Biomarker Analysis of Blood Samples
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All blood samples were obtained by venipuncture into a vacutainer containing clot activator. Following inversion and clotting, the blood sample was centrifuged (Biosan LMC-3000 version V.5AD, Riga, Latvia) for ten minutes at 3000 rpm and transferred into Eppendorf tubes and stored frozen at −80 °C until further analysis. Follicle stimulating hormone (FSH), LH, progesterone, 17β estradiol and IL-6 were measured with a COBAS E411 (Roche Diagnostics, GmbH, Mannheim, Germany), using electrochemiluminescence immunoassay (ECLIA) technology. TNF-α was measured with an IMMULITE 1000 system (Siemens Healthineers AG, Munich, Germany) using chemiluminescent enzymatic immunoassay. Finally, CK, myoglobin, LDH, CRP, ALT and AST were analyzed in a Beckman AU400 Clinical Biochemistry analyzer (Beckman Coulter Inc., Brea, CA, USA). Reactive was calibrated following internal laboratory calibration protocols, and controls were assessed after calibration. Coefficients of variation reported by the laboratory were 4.70% for FSH, 5.15% for LH, 4.85% for 17- ß estradiol, 6.35% for progesterone, 4.10% for IL-6, 6.50% for TNF-α, 6.43% for CK, 4.17% for myoglobin, 5.72% for LDH, 6.40% for CRP, 7.19% for AST, and 7.32% for ALT.
3
Sex Hormone Profiling for Ultradian Rhythm
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To avoid ultradian rhythm variations (Janse de Jonge 2003), blood sample tests were done at the same time for all volunteers, between 8 and 10 a.m. They were obtained with venipuncture into a vacutainer containing clot activator. Following inversion and clotting, the whole blood was centrifuged (Biosan LMC-3000 version V.5AD) for 10 min at 3000 rpm. After that, serum was transferred into Eppendorf tubes and stored frozen at -80 ºC until further analysis. Within 1-15 days after testing, the serum samples were delivered to the clinical laboratory of the Spanish National Centre of Sport Medicine (Madrid, Spain) to determine sex hormones to verify hormonal profiles. Total E2, progesterone, folliclestimulating hormone (FSH) and luteinizing hormone (LH) were measured via ADVIA Centaur ® solid-phase competitive chemiluminescent enzymatic immunoassay (Siemens City, Germany). Inter-and intra-assay coefficients of variation (CV) reported by the laboratory for each variable were, respectively: 11.9% and 8.5% at 93.3 pg/ml and 6.8% and 4.7% at 166 pg/ml for E2; 23.1% and 11.8% at 0.7 ng/ml and 5.2% and 2.5% at 9.48 ng/ml for progesterone, 5.3% and 1.8% at 1.2 mIU/ml for FSH and 5.2% and 1.8% at 0.54 mIU/ml for LH.
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