Fitc cd14 clone m5e2

Mo‐DCs and Mo‐M were detached using non‐enzymatic cell dissociation solution (Sigma Aldrich) and washed once in FACS buffer prior to staining for flow cytometry. Antibodies used were; FITC‐CD14 clone M5E2 (1:10), APC‐HLA‐DR clone L243 (1:50), PE‐CD80 clone L307.4 (1:15), PE‐CD86 clone IT2.2 (1:15), PE‐CD83 clone HB15e (1:15), PE‐CD1a clone HI149 (1:15), APC‐ CD206 clone 19.2 (1:20), APC‐CD209 clone DCN46 (1:15) and APC‐CD33 clone WM53 (1:10), all from BD Biosciences (San Jose, Ca, USA). All analyses were performed using 7AAD as a cell death discriminator. Cells were analyzed using a FACS Calibur (BD Biosciences). All antibodies and dilutions were optimized previously [35, 36], adhering to the guidelines by Cossarizza et al [37].