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2 protocols using cd11c apc antibodies

1

Andrographolide Modulates Inflammatory Pathways

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OVA, LPS and Andrographolide were purchased from Sigma-Aldrich (St. Louis, MO). Water-soluble Andrographolide sulfonate (Xi-Yan-Ping Injection) was provided by Jiangxi Qingfeng Pharmaceutical Co., Ltd. ELISA kits for TNF-α, IL-6, IL-4 and IL-1β were purchased from Dakewei (Beijing, China). Anti-phosphorylation of p65 and anti-p-p65 were purchased from Cell Signaling Technology (Beverly, MA). Anti-NLRP3 and anti-CASP1 (3345-1) were purchased from Epitomics. Anti-ASC and anti-Actin (sc-1616) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-mouse CD3-FITC, CD11b-PE and CD11c-APC antibodies were bought from eBioscience. GTVisin™ anti-mouse/anti-rabbit immunohistochemical analysis KIT was purchased from Gene Company (Shanghai, China). All other chemicals were obtained from Sigma-Aldrich (St. Louis, MO).
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2

Andrographolide Sulfonate Anti-Inflammatory Effects

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Andrographolide sulfonate (trade name: Xi-Yan-Ping Injection) was provided by Jiangxi Qingfeng Pharmaceutical Co., Ltd. (Ganzhou, China). LPS (from Escherichia coli (0111:B4)) was purchased from Sigma-Aldrich (St. Louis, MO, USA). ELISA kits for TNF-α, IL-6 and IL-1β were purchased from Dakewei (Beijing, China). Anti-phosphorylation of c-Jun N-terminal kinase (JNK) (Thr183/Tyr185), anti-phosphorylation of ERK1/2 (Thr202/Tyr204), anti-phosphorylation of p38 (Thr180/Tyr182), anti-phosphorylation of p65 were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-mouse CD3-FITC, CD11b-PE and CD11c-APC antibodies were bought from eBioscience. (San Diego, CA, USA) GTVisin™ anti-mouse/anti-rabbit immunohistochemical analysis KIT was purchased from Gene Company (Shanghai, China). All other chemicals were obtained from Sigma-Aldrich (St. Louis, MO, USA).
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