Environmental control chamber

Manufactured by Okolab

Sourced in Italy

The Environmental control chamber is a laboratory equipment designed to provide a controlled and stable environment for various applications. Its core function is to maintain precise temperature, humidity, and atmospheric conditions within the chamber, allowing researchers to create and study specific environmental conditions.

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Lab products found in correlation

Icrf 193, by Santa Cruz Biotechnology (1 mentions) Ti e microscope, by Nikon (1 mentions) Ibitreat dishes, by Ibidi (1 mentions) Silicone culture insert, by Ibidi (1 mentions) Volocity software, by PerkinElmer (1 mentions) Ti microscope, by Nikon (1 mentions) Heparin, by Merck Group (1 mentions) Glass bottom microwell dishes, by MatTek (1 mentions)

Close spelling variants of this product

Environmental chamber

4 protocols using environmental control chamber

Live-cell Imaging of Topoisomerase Inhibitors

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For live-cell imaging, cells were grown on 35-mm ibiTreat µ-dishes (Ibidi). Time-lapse Z-stack images were captured on a Nikon TiE microscope equipped with a 60× phase-contrast objective, 1.4-NA, Perfect Focus mechanism, Yokogawa CSU-W1 spinning disk, and Flash 4.0 sCMOS camera. Cells were imaged in the regular growth medium; 37°C and 5% CO₂ was maintained using an environmental control chamber (Okolab). 10 µM ICRF-193 (Santa Cruz Biotechnology) and 500 µM dexrazoxane (TCI America) were added shortly before initiation of imaging. Images were acquired with NIS Elements software. Image processing (maximum-intensity projection, background subtraction, and stack combining) was done in Fiji.

Potapova T.A., Unruh J.R., Yu Z., Rancati G., Li H., Stampfer M.R, & Gerton J.L. (2019). Superresolution microscopy reveals linkages between ribosomal DNA on heterologous chromosomes. The Journal of Cell Biology, 218(8), 2492-2513.

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Cell Migration Assay in U2OS Cells

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U2OS cells were seeded and cultured in a Silicone Culture-Insert (ibidi; Matinsried, Germany) set into a 35 mm µ-Dish (ibidi) and grown for at least 24 hours. Once cells reached confluence, the insert was removed and cells were washed once with fresh media, leaving the cells to migrate for the indicated times. For the rescue experiments, U2OS cells were transiently transfected with USP45 WT or KO. Imaging was performed on a Nikon Ti microscope (Nikon, Tokyo, Japan) fitted with an OKOlab environmental control chamber (Okolab, Pozzuoli, Italy), 20x 0.45NA objective, Nikon 421 PerfectFocus System, and a Photometrics Cascade II camera (Tucson, AZ, USA) and NIS Elements software. Images were then processed using FiJi software. Measurements were made by drawing a line between the edges and measuring the distance at the indicated time points. Kymograph analyses were conducted using Volocity software (Perkin Elmer; 425 Waltham, MA, USA) to measure the velocity of movement over the time.

Conte C., Griffis E.R., Hickson I, & Perez-Oliva A.B. (2018). USP45 and Spindly are part of the same complex implicated in cell migration. Scientific Reports, 8, 14375.

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Live Imaging of Mouse Embryo Development

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Embryos were collected at indicated developmental stages following timed matings of mice maintained in a 12-hour light-dark cycle according to previously described protocols(Behringer, 2014 ). For live imaging experiments, embryos were transferred to glass-bottom microwell dishes (MatTek) and maintained in an environmental control chamber (OKOlab) and imaged every 15 minutes for 9–12 hours, sampling a depth 60 μm at 5 μm steps. For inhibitor incubations, embryos were transferred to EmbryoMax Advanced KSOM medium with the specified concentrations of inhibitors and incubated for at least 20 minutes before placing the glass-bottom dish in the environmental control chamber. For FGF4 addition experiments, zonae pellucidae were removed by brief incubation in Acid Tyrode’s solution and FGF4 was co-administered with 1 μg/ml heparin (Sigma Aldrich).

Pokrass M.J., Ryan K.A., Xin T., Pielstick B., Timp W., Greco V, & Regot S. (2020). Cell Cycle-Dependent ERK Signaling Dynamics Direct Fate Specification in the Mammalian Preimplantation Embryo. Developmental cell, 55(3), 328-340.e5.

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Analyzing Pseudopod Dynamics in Ovarian and Breast Cancer Cells

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A2780 ovarian carcinoma cells or MDA-MB-231 cells were plated onto the fibroblast-derived ECM in 6-well plate at a density of 1 × 10⁵ cells per well. After 2 h, indicated stimuli were added in the medium and 3 h later the time lapse started. Pictures of the cells were taken every 5 min over a 22 h period with a 10 × objective on a Nikon Eclipse Ti microscope equipped with a CoolSNAP HQ CCD camera (Photometrics). Cells were maintained at 37 °C and 5% CO₂ for the duration of the experiment (environmental control chamber, Okolab). The pseudopod length analysis was carried out with ImageJ. The pseudopod length was measured from the nucleus to the frontal tip of the cell. For each well, six fields were recorded and for each of them the length of the pseudopod of 30 cells measured (=180 cells measured for each experimental condition in each replicate experiment). For the experiment where iNF- and iCAF-generated CM was used as stimulus (Fig. 1), only MDA-MB-231 cells were used because these cells grow in the same medium (DMEM) as the fibroblasts.

Hernandez-Fernaud J.R., Ruengeler E., Casazza A., Neilson L.J., Pulleine E., Santi A., Ismail S., Lilla S., Dhayade S., MacPherson I.R., McNeish I., Ennis D., Ali H., Kugeratski F.G., Al Khamici H., van den Biggelaar M., van den Berghe P.V., Cloix C., McDonald L., Millan D., Hoyle A., Kuchnio A., Carmeliet P., Valenzuela S.M., Blyth K., Yin H., Mazzone M., Norman J.C, & Zanivan S. (2017). Secreted CLIC3 drives cancer progression through its glutathione-dependent oxidoreductase activity. Nature Communications, 8, 14206.

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