S100a8 cre

Manufactured by Jackson ImmunoResearch

S100a8-Cre is a Cre recombinase transgenic mouse line that expresses Cre under the control of the S100a8 gene promoter. The S100a8 gene encodes a calcium-binding protein that is expressed in myeloid cells. This mouse line can be used to target Cre-mediated recombination specifically in myeloid cells.

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Lab products found in correlation

Lyz2 cre, by Jackson ImmunoResearch (1 mentions) Ptpn6fl fl, by Jackson ImmunoResearch (1 mentions) C57bl 6j mice, by Jackson ImmunoResearch (1 mentions) Rosa26lsl yfp, by Jackson ImmunoResearch (1 mentions) Pc g5 tdt, by Jackson ImmunoResearch (1 mentions)

4 protocols using s100a8 cre

Genetic Manipulation of Mouse Immune Cells

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C57BL/6J mice were purchased from The Jackson Laboratory (Bar Harbor, ME) and maintained under specific pathogen–free conditions in the animal facilities at the University of Iowa. The mice were reared on a 12-hour light/dark cycle at a constant temperature of 22° ± 1°C. Ptpn6^spin (MMRRC Repository, stock no. 015198-UCD) (7 (link)), Ptpn6^fl/fl (Jackson Labs, stock no. 008336) (45 (link)),
Cd47^−/− (Jackson Labs, stock no. 003173) (43 (link)), Lyz2-Cre (Jackson Labs, stock no. 004781) (46 (link)), and S100a8-Cre (Jackson Labs, stock no. 021614) (47 (link)) mice have been described previously.
Cd47^−/− × Ptpn6^spin DKO mice were generated by breeding
Cd47^−/− and Ptpn6^spin mice. Ptpn6^Δmyeloid and Ptpn6^ΔPMN mice were generated by breeding Ptpn6^fl/fl mice with Lyz2-Cre and S100a8-Cre mice, respectively. All experimental procedures were approved by the Office of Animal Resources of the University of Iowa (Institutional Animal Care and Use Committee, protocol no. 0032004).

Mazgaeen L., Yorek M., Saini S., Vogel P., Meyerholz D.K., Kanneganti T.D, & Gurung P. (2023). CD47 halts Ptpn6-deficient neutrophils from provoking lethal inflammation. Science Advances, 9(1), eade3942.

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Genetically Engineered Mouse Models for Breast Cancer

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CD45.1⁺, Irf8^fl/fl, OT-I, and S100a8^Cre mice were purchased from Jackson Laboratory. IRF8^EGFP and MafB^iCre mice were generated as previously reported (Wang et al., 2014 (link); Wu et al., 2016 (link)). LSL-USA mice were generated in the laboratory of Dr. James Moon (Zhang et al., 2019 ). MMTV-PyMT, CD11c^Cre and Rosa26^LSL-YFP were in the lab as previously reported (Franklin et al., 2014 (link)). All mice used in these studies were on the C57BL/6 background and were female given the focus on the mammary gland and PyMT breast cancer. All mice were maintained under specific pathogen-free conditions, and animal experimentation was conducted in accordance with procedures approved by the Institutional Animal Care and Use Committee of MSKCC.

Nixon B.G., Kuo F., Ji L., Liu M., Capistrano K., Do M., Franklin R.A., Wu X., Kansler E.R., Srivastava R.M., Purohit T.A., Sanchez A., Vuong L., Krishna C., Wang X., Morse HC I.I.I., Hsieh J.J., Chan T.A., Murphy K.M., Moon J.J., Hakimi A.A, & Li M.O. (2022). Tumor-associated macrophages expressing the transcription factor IRF8 promote T cell exhaustion in cancer. Immunity, 55(11), 2044-2058.e5.

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Genetically Engineered Mouse Models

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All in vivo mouse experimental procedures were performed under the Sloan Kettering Institute (SKI) Institutional Animal Care and Utilization Committee (IACUC) - approved protocol 07-06-008. Mice were housed in specific pathogen-free animal facilities. Only female mice were used in this study. Littermates were used in all experiments, when possible, otherwise age-matched cage mates were used. Itgax-Cre, S100a4-Cre, S100a8-Cre, Rosa26^LSL-YFP, Cdh1^mCFP and GCaMP5 (also known as PC-G5-tdT) mice were obtained from Jackson Laboratories. Gzmc^tdT-T2A-iCre mice were generated in the Li lab^{32 (link)}. IL-15^2A-eGFP mice were previously described^{28 (link)}. Il15^fl mice were generated in the Ikuta lab and will be reported elsewhere. Briefly, the Il15^fl allele was established by flanking exon 5 with two loxP sequences by standard homologous recombination in embryonic stem cells (schematic provided in Extended Data Fig. 6c).

Kansler E.R., Dadi S., Krishna C., Nixon B.G., Stamatiades E.G., Liu M., Kuo F., Zhang J., Zhang X., Capistrano K., Blum K.A., Weiss K., Kedl R.M., Cui G., Ikuta K., Chan T.A., Leslie C.S., Hakimi A.A, & Li M.O. (2022). Cytotoxic Innate Lymphoid Cells Sense Cancer Cell-Expressed Interleukin-15 to Suppress Human and Murine Malignancies. Nature immunology, 23(6), 904-915.

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Genetically Modified Mouse Models

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S100a8-Cre-ires/eGFP mice (48 (link)), hereafter called S100a8-Cre, were purchased from The Jackson Laboratory. Inhba^fl/fl mice were genotyped as described (49 (link)). Acvr1b^fl/fl (50 (link)) and Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo} mice (51 (link)), herein called Rosa-Tomato^fl/fl, were kindly provided by Dr. G. Xanthou and Dr. A. Klinakis [Biomedical Research Foundation of the Academy of Athens (BRFAA), Athens, Greece], respectively. S100a8-Cre were crossed to Inhba^fl/fl to generate S100a8-Cre/Inhba^fl/fl, to Acvr1b^fl/fl to generate S100a8-Cre/Acvr1b^fl/fl, and to Rosa-Tomato^fl/fl to generate S100a8-Cre/Rosa-Tomato^fl/fl mice, all maintained in C57BL/6 background. S100a8-Cre, Inhba^fl/fl, Acvr1b^fl/fl, and Rosa-Tomato^fl/fl mice were used as controls. In all experiments, 10-12-week-old female mice, housed at the animal house facility of the BRFAA as reported (52 (link)), were used.

Divolis G., Synolaki E., Doulou A., Gavriil A., Giannouli C.C., Apostolidou A., Foster M.L., Matzuk M.M., Skendros P., Galani I.E, & Sideras P. (2024). Neutrophil-derived Activin-A moderates their pro-NETotic activity and attenuates collateral tissue damage caused by Influenza A virus infection. Frontiers in Immunology, 15, 1302489.

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