1 2 dipalmitoyl sn glycero 3 phosphoethanolamine

All chemical materials used in this study were analytical grade unless stated otherwise. 1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPC), cholesterol, tween-20, dimethyldioctadecylammonium bromide (DDAB), complete freund’s adjuvant (CFA), incomplete freund’s adjuvant (IFA), and o-phenylenediamine dihydrochloride (OPD) tablets were purchased from Sigma-Aldrich. p-methylbenzhydrylamine (MBHA)^. HCl resin was purchased from Novabiochem. N, N’-dimethylformamide (DMF), trifluoroacetic (TFA) acid, acetonitrile, protected (tert-butyloxycarbonyl) Boc-amino acids and 1-[bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium3-oxid hexafluorophosphate (HATU) were purchased from Mimotopes. Chloroform, methanol, skim milk powder, and other solvents were purchased obtained from Merck. Goat antimouse IgG (H+L)-HRP conjugate secondary antibody was purchased from Bio-Rad, Australia).
Analytical reverse-phase high performance liquid chromatography (RP-HPLC) and preparative RP-HPLC were performed on a Shimadzu instrument using Vydac analytical (C4 and C18) and preparative (C4 and C18) columns. HPLC solvent A: 0.1% TFA in water and solvent B: 0.1% TFA in acetonitrile: water (90:10); flow rate: 1 mL/min for analytical RP-HPLC and 20 mL/min for preparative RP-HPLC; detection: UV absorbance at 214 nm.

E. pacifica was purchased from Kawashu Inc. (Iwate, Japan) and Kokuyou Inc. (Iwate, Japan), and was originally harvested from the Pacific Ocean near Iwate, Japan. E. superba was purchased from HiromatsuKyu Fishery Co.,Ltd (Fukuoka, Japan). Balanus rostratus Hoek was purchased directly from a fisherman culture in Ofunato, Iwate, Japan. Marsupenaeus japonicus was purchased from a local fisherman in Aichi, Japan. Fatty acids, HEPEs, hydroxyeicosatetraenoic acids (HETEs) and hydroxy docasahexaenoic acids (HDoHEs) were purchased from Cayman Chemical (Ann Arbor, MI). Dipalmitoyl Phosphocholine, 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine, glyceryl tripalmitate, DL-a-palmitin and 1-palmitoyl-sn-glycero-3-phosphocoline were purchased from Sigma-Aldrich (St. Louis, MO).