6210 tof mass spectrometer

Manufactured by Agilent Technologies

Sourced in Germany, United States

The 6210 TOF mass spectrometer is a high-performance, time-of-flight mass spectrometer designed for accurate mass measurements. It utilizes a time-of-flight analyzer to separate and detect ionized molecules based on their mass-to-charge ratio. The instrument is capable of providing precise mass data for a wide range of applications.

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6210 ESI/TOF mass spectrometer (12 citations) Agilent 6210 ESI/TOF mass spectrometer (11 citations) Spectrometers (10 citations) 6210 LC-TOF mass spectrometer (6 citations) Agilent-6210-LC/TOF mass spectrometer (5 citations) Agilent 6210 TOF mass spectrometer (4 citations) Agilent 6210 LC/MSD TOF mass spectrometer (3 citations) 6210 TOF LC/MS mass spectrometer (2 citations) 6210 ESI TOF LCMS mass spectrometer (2 citations) 6210 time-of-flight mass spectrometer (TOF–MS) (2 citations)

11 protocols using 6210 tof mass spectrometer

Metabolome Profiling by CE-TOFMS

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CE-TOFMS was performed on an Agilent CE Capillary Electrophoresis System equipped with an Agilent 6210 TOF mass spectrometer, Agilent 1100 isocratic HPLC pump, Agilent G1603A CE-MS adapter kit, and Agilent G1607A CE-ESI-MS sprayer kit (Agilent Technologies, Waldbronn, Germany). Metabolome measurements were performed at Human Metabolome Technologies as previously described (Soga and Heiger 2000 (link); Soga et al. 2002 (link); Soga et al. 2003 (link)). For details of the measurement, see supporting information.

Tani T., Okamoto K., Fujiwara M., Katayama A, & Tsuruoka S. (2019). Metabolomics analysis elucidates unique influences on purine / pyrimidine metabolism by xanthine oxidoreductase inhibitors in a rat model of renal ischemia-reperfusion injury. Molecular Medicine, 25, 40.

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Spectroscopic Analysis of Organic Compounds

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Optical rotations were recorded on a Perkin-Elmer 241 polarimeter. UV spectra were measured on a Shimadzu UV 2201 spectrophotometer. IR spectra were recorded on a Bruker IFS 55 spectrometer. Bruker AV-400 and AV-600 spectrometers were used in the NMR experiments. Chemical shift values were expressed in δ (ppm) using the peak signals of the solvent DMSO-d₆ (δ_H 2.50 and δ_C 39.51) as references, and coupling constants (J in Hz) were given in parentheses. HRESIMS data were acquired on an Agilent 6210 TOF mass spectrometer. Silica gel GF₂₅₄ prepared for TLC was purchased from Qingdao Marine Chemical Factory (Qingdao, China). Silica gel (200–300 mesh, Qingdao Marine Chemical Factory, Qingdao, China), Sephadex LH-20 (Pharmacia, USA), and octadecyl Silica gel (Merck Chemical Company Ltd., German) were used for column chromatography (CC). RP-HPLC separations were conducted using an LC-6AD liquid chromatograph and a SPD-20A UV detector (Shimadzu, Kyoto, Japan) with a RP-C₁₈ column (250 × 20 mm, 120 Å, 5 μm, YMC Co. Ltd.).

Sun C.P., Qiu C.Y., Zhao F., Kang N., Chen L.X, & Qiu F. (2017). Physalins V-IX, 16,24-cyclo-13,14-seco withanolides from Physalis angulata and their antiproliferative and anti-inflammatory activities. Scientific Reports, 7, 4057.

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LC/ESI-MS Protein Analysis Protocol

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LC/ESI-MS was performed on a 6210 TOF mass spectrometer coupled to a HPLC system (1100 series, Agilent Technologies). All solvents used were HPLC grade; the HPLC mobile phases were A: H2O 95%, ACN 5%, TFA 0.03%, B: ACN 95%, H2O 5%, TFA 0.03%. Protein samples were desalted on-line on a C8 reverse phase micro-column (Zorbax 300SB-C8, 5μm, 5x0.3mm, Agilent Technologies) for 3 minutes at 100 μl/min with 100% of mobile phase A, then eluted at 50 μl/min with 70% of mobile phase B. MS acquisition was carried out in the positive ion mode in the 300–3000 m/z range and the data processed with MassHunter software (v. B.02.00, Agilent Technologies). The mass spectrometer was calibrated with tuning mix (ESI-L, Agilent Technologies). The mass spectrometer settings were the following: gas temperature (azote) 300°C, drying gas (azote) 7 liters/min, nebulizer gas (azote): 10 psig, Vcap: 4 kV, fragmentor: 250 V, skimmer: 60 V, Vpp (octopole RF): 250 V.

Pellegrini E., Signor L., Singh S., Boeri Erba E, & Cusack S. (2017). Structures of the inactive and active states of RIP2 kinase inform on the mechanism of activation. PLoS ONE, 12(5), e0177161.

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HPLC-TOFMS Analysis of Metabolites

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Analysis was carried out with a method reported by McMahen et al. (2015) (link). Briefly, an Agilent 1100 HPLC interfaced with an Agilent 6210 (TOF) mass spectrometer fitted with an electrospray ionization (ESI) source was used. The HPLC was performed with a Zorbax Eclipse Plus C18 column (2.1 × 50 mm, 3.5 μm, Agilent Technologies) fitted with a Phenomenex guard column (Torrance, CA). The method consisted of the following: 0.2 mL/min flow rate; at 30 °C; mobile phases: A: ammonium formate buffer (0.4 mM) and DI water:methanol (95:5 v/v), and B: ammonium formate (0.4 mM) and methanol:DI water (95:5 v/v); gradient: 0–5 min a linear gradient from 50:50 A:B to 100% B; 5–15 min, 100% B; 15–18 min re-equilibration to 50% A and 50% B.

Vasylieva N., Barnych B., Wan D., El-Sheikh E.S., Nguyen H.M., Wulff H., McMahen R., Strynar M., Gee S.J, & Hammock B.D. (2017). Hydroxy-fipronil is a new urinary biomarker of exposure to fipronil. Environment international, 103, 91-98.

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Spectroscopic Analysis of Natural Compounds

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Optical rotations were measured with a PerkinElmer 241 polarimeter. UV
spectra were recorded on a Shimadzu UV 2201 spectrophotometer, and IR
(4000–400 cm⁻¹) spectra (KBr pellets) were recorded
on a Bruker IFS 55 spectrometer. NMR experiments were performed on Bruker
ARX-300 and AV-600 spectrometers. Chemical shifts are stated relative to TMS and
expressed in δ values (ppm), with coupling constants reported in Hz.
HRESIMS were obtained on an Agilent 6210 TOF mass spectrometer. Silica gel GF254
prepared for TLC and silica gel (200–300 mesh) for column chromatography
(CC) were obtained from Qingdao Marine Chemical Factory (Qingdao,
People’s Republic of China). Sephadex LH-20 was a product of Pharmacia
(Uppsala, Sweden). Octadecyl silica gel (ODS) was purchased from Merck Chemical
Company Ltd. RP-HPLC separations were conducted using a LC-6AD liquid
chromatograph with a YMC Pack ODS-A column (250 × 20 mm, 5
μm, 120 Å) and SPD-10A VP UV/vis detector.
All reagents were HPLC or analytical grade and were purchased from Tianjin Damao
Chemical Company (Tianjin, People’s Republic of China). Spots were
detected on TLC plates under UV light or by heating after spraying with
anisaldehyde/H₂SO₄ reagent.

Chen L.X., Xia G.Y., He H., Huang J., Qiu F, & Zi X.L. (2016). New withanolides with TRAIL-sensitizing effect from Physalis pubescens L.. RSC advances, 6(58), 52925-52936.

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Analytical Characterization of Natural Compounds

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Optical rotations were measured with a PerkinElmer 241 polarimeter. UV spectra were collected in a Shimadzu UV 2201 spectrophotometer. ECD spectra were recorded on a Bio-Logic Science MOS-450 spectrometer. IR spectra were obtained on a Bruker IFS 55 spectrometer. NMR experiments were performed on Bruker AV-400 and AV-600 spectrometers. Chemical shifts are reported as δ (ppm) related to the solvent pyridine-d₅ (δ_H 7.58 and δ_C 135.91) as references, and coupling constants (J values) are given in Hz. HRESIMS data were obtained on an Agilent 6210 TOF mass spectrometer. Silica gel GF₂₅₄, obtained from Qingdao Marine Chemical Factory, was used for TLC. Sephadex LH-20 for gel-permeation chromatography was obtained from Pharmacia. Column chromatography (CC) was performed on silica gel (200–300 mesh, Qingdao Marine Chemical Factory) and octadecyl silica gel (Merck Chemical Company Ltd., Darmstadt, Germany). RP-HPLC separations were conducted using an LC-6AD liquid chromatograph, SPD-20A UV detector (Shimadzu, Kyoto, Japan), equipped with a YMC Pack ODS-A column (250 × 20 mm, 120 Å, 5 μm).

Zhang M., Zhang B., Guang C., Jiang B., He X., Cao S., Ding L., Kang N., Chen L, & Qiu F. (2020). New withanolides from Physalis minima and their cytotoxicity against A375 human melanoma cells. RSC Advances, 10(38), 22819-22827.

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Chiral Compound Characterization Protocol

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The melting point (uncorrected) was determined on an X-4 digital display micromelting point apparatus. Optical rotations were measured with a Perkin-Elmer 241 polarimeter. UV spectra were recorded on a Shimadzu UV 2201 spectrophotometer. IR spectra were recorded on a Bruker IFS 55 spectrometer. CD spectra were recorded on a Bio-Logic Science MOS-450 spectrometer. NMR experiments were performed on Bruker ARX-300 and AV-600 spectrometers. HRESIMS were obtained on an Agilent 6210 TOF mass spectrometer. Silica gel GF254 prepared for TLC and silica gel (200–300 mesh) for column chromatography were obtained from Qingdao Marine Chemical Factory (Qingdao, People’s Republic of China). Octadecyl silica gel was purchased from Merck Chemical Company Ltd. RP-HPLC separations were conducted using an LC-6AD liquid chromatograph with a YMC Pack ODS-A column (250 × 20 mm, 5 μm, 120 Å) and an SPD-10A VP UV/VIS detector. Analysis and chiral purifications of racemates of 1–4 were carried out on a Chiralpak IE column (150 mm × 4.6 mm, 5 μm; Daicel Chemical Industries, Ltd). All reagents were of HPLC or analytical grade and were purchased from Tianjin Damao Chemical Company. Spots were detected on TLC plates under UV light or by heating after spraying with anisaldehyde-H₂SO₄.

Xia G.Y., Sun D.J., Ma J.H., Liu Y., Zhao F., Owusu Donkor P., Ding L.Q., Chen L.X, & Qiu F. (2017). (+)/(−)-Phaeocaulin A-D, four pairs of new enantiomeric germacrane-type sesquiterpenes from Curcuma phaeocaulis as natural nitric oxide inhibitors. Scientific Reports, 7, 43576.

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N-Glycan Analysis by Nano-HPLC-Chip-TOF-MS

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N-glycans were analyzed using an Agilent (Santa Clara, CA) 6200 series nanoHPLC-chip-TOF-MS, consisting of an autosampler, which was maintained at 8°C, a capillary loading pump, a nanopump, HPLC-chip-MS interface and an Agilent 6210 TOF mass spectrometer [34 (link)]. The microfluidic chip (glycan chip II, Agilent) contained a 9 × 0.075 mm i.d. enrichment column coupled to a 43 × 0.075 mm i.d. analytical column, both packed with 5 μm porous graphitized carbon (PGC). N-glycans from IgG were reconstituted in 50 μL of water and 1 μL of sample was used for injection. Upon injection, the sample was loaded onto the enrichment column using 3% ACN containing 0.1% formic acid (FA, Fluka, St. Louis, MO). After the analytical column was switched in-line, the nano-pump delivered a gradient of 3%ACN with 0.1% FA (solvent A) and 90% ACN with 0.1% FA (solvent B). The mass spectrometer was operated in the positive mode, and ions were scanned over a mass range from m/z 400-3000.

Ruhaak L.R., Barkauskas D.A., Torres J., Cooke C.L., Wu L.D., Stroble C., Ozcan S., Williams C.C., Camorlinga M., Rocke D.M., Lebrilla C.B, & Solnick J.V. (2015). The Serum Immunoglobulin G Glycosylation Signature of Gastric Cancer. EuPA open proteomics, 6, 1-9.

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Mass Spectrometry Analysis of Proteins

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To assess the mass of the different proteins, a 6210 TOF mass spectrometer coupled to a HPLC system (1100 series, Agilent Technologies) was used. The mass spectrometer was calibrated with tuning mix (ESI‐L, Agilent Technologies). The following instrumental settings were used: gas temperature (nitrogen) 300°C, drying gas (nitrogen) 7 l/min, nebulizer gas (nitrogen) 10 psi, Vcap 4 kV, fragmentor 250 V, skimmer 60 V, and Vpp (octopole RF) 250 V. The HPLC mobile phases were prepared with HPLC‐grade solvents. Mobile phase A composition was: H2O 95%, ACN 5%, and TFA 0.03%. Mobile phase B composition was as follows: ACN 95%, H2O 5%, and TFA 0.03%. Each protein was diluted to 5 uM using mobile phase A. Four μl of each sample (20 pmol) was injected into HPLC system MS analysis and was first desalted online for 3 min with 100% of mobile phase A (flow rate of 50 μl/min), using a C8 reverse phase micro‐column (Zorbax 300SB‐C8, 5 μm, 5 × 0.3 mm, Agilent Technologies). The sample was then eluted with 70% of mobile phase B (flow rate of 50 μl/min), and MS spectra were acquired in the positive ion mode in the 300–3,000 m/z range (Boeri Erba et al, 2018 (link)). Data were processed using MassHunter software (v. B.02.00, Agilent Technologies) and GPMAW software (v. 7.00b2, Lighthouse Data, Denmark).

Laroussi H., Juarez‐Martinez A.B., Le Roy A., Boeri Erba E., Gabel F., de Massy B, & Kadlec J. (2023). Characterization of the REC114‐MEI4‐IHO1 complex regulating meiotic DNA double‐strand break formation. The EMBO Journal, 42(16), e113866.

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Plasma Oligosaccharide Analysis by nanoHPLC-TOF-MS

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Plasma derived MO were analyzed using an Agilent (Santa Clara, CA) 6200 series nanoHPLC-chip-TOF-MS, consisting of an autosampler, which was maintained at 8 °C, a capillary loading pump, a nanopump, HPLC-chip-MS interface and an Agilent 6210 TOF mass spectrometer. The chip (Glycan Chip II, Agilent) contained a 9 × 0.075 mm i.d. enrichment column coupled to a 43 × 0.075 mm i.d. analytical column; both packed with 5 μm porous graphitized carbon (PGC). Plasma oligosaccharide samples were reconstituted in 50 μL of water prior to analysis; 5 μL of sample was used for injection. Upon injection, the sample was loaded onto the enrichment column using 3% ACN containing 0.1% formic acid (FA, Fluka, St. Louis, MO). The analytical column was then switched on-line so that the nano-pump delivered a gradient of 3% ACN with 0.1% FA (solvent A) to 90% ACN with 0.1% FA (solvent B). The gradient increased from 0% B to 33% B over 13 minutes, followed by an increase to 36% B at 16.5 minutes and 100% B at 17 minutes. The column was then washed at 100% B for 5 minutes, followed by 10 minutes reequilibration at 0% B. Positive ions were generated and mass spectra were acquired over a mass window of 400 m/z to 3000 m/z.

Ruhaak L.R., Stroble C., Underwood M.A, & Lebrilla C.B. (2014). Detection of milk oligosaccharides in plasma of infants. Analytical and bioanalytical chemistry, 406(24), 5775-5784.

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