HAECs and HASMCs were grown until confluent and treated with ucOCN (10 ng/ml) or with control media for 2, 5, 10, and 30 min. The MILLIPLEX MAP 9‐plex Multi‐Pathway Magnetic Bead Signaling Kit (48‐680MAG; Merck Millipore) was performed according to the manufacturers’ instructions to detect changes in phosphorylated ERK/MAP kinase 1/2 (Thr185/Tyr187), AKT (Ser473), STAT3 (Ser727), JNK (Thr183/Tyr185), p70s6 kinase (Thr412), NF‐kB (Ser536), STAT5A/B (Tyr694/699), CREB (Ser133), and p38 (Thr180/Tyr182) in cell lysates using Luminex® xMAP® technology. Phosphorylated‐ and total‐mTOR (Ser2448) were also measured in the lysate samples using the Luminex system (48‐625MAG, Merck Millipore).
Milliplex map 9 plex multi pathway magnetic bead signaling kit
Manufactured by Merck Group
The MILLIPLEX MAP 9-plex Multi-Pathway Magnetic Bead Signaling Kit is a multiplex assay designed to simultaneously quantify the levels of nine different signaling proteins in a single sample. The kit utilizes magnetic beads coated with capture antibodies specific to each target analyte, allowing for the measurement of multiple analytes in a single well.
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Lab products found in correlation
2 protocols using milliplex map 9 plex multi pathway magnetic bead signaling kit
1
Phosphorylation Dynamics of Key Signaling Pathways
2
Multiplex Signaling Pathway Analysis
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The MILLIPLEX MAP 9‐plex Multi‐Pathway Magnetic Bead Signaling Kit (catalog no. 48‐680MAG; Merck Millipore) was performed per the manufacturers’ instructions to detect changes in phosphorylated ERK/MAP kinase 1/2 (Thr185/Tyr187), AKT (Ser473), STAT3 (Ser727), JNK (Thr183/Tyr185), p70s6 kinase (Thr412), NFkB (Ser536), STAT5A/B (Tyr694/699), CREB (Ser133), and p38 (Thr180/Tyr182) in cell lysates using the Luminex® xMAP® technology (48‐680MAG; Milliplex™; Merck Millipore).
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