Fungizone

L1CAM positive SKOV3ip cells were kindly provided by P. Altevogt (German Cancer Research Center, Heidelberg, Germany). Cells were established from ascitic fluid of a nu/nu mouse that was previously injected with SKOV3 cells [33 (link)]. SKOV3ip cells were maintained in DMEM medium at 37 °C. IGROV1 cells were a kind gift by Dr. Cristina Müller (Center for Radiopharmaceutical Sciences, Paul Scherrer Institute) and were maintained in RPMI 1640 medium at 37 °C. Both cell media were supplemented with 10% fetal calf serum (FCS), 2 mM glutamine, 100 units/ml penicillin, 100 μg/ml streptomycin and 0.25 μg/ml fungizone (BioConcept, Allschwil, Switzerland). The two cell lines were cultivated in a humidified atmosphere containing 5% CO₂. Cell lines were authenticated by STR profiling (Department of Molecular Medicine, Aarhus University Hospital, Denmark; DSMZ Authentication Service, Braunschweig, Germany) and were mycoplasma free (Mycoplasma test kit, AppliChem GmbH, Darmstadt, Germany). L1CAM expression in both cell lines was confirmed by flow cytometry (Additional file 1: Figure S1). The cell lines did not require ethics approval.
Chimeric monoclonal antibody mAb chCE7 (human κ light chain and human γ1 heavy chain) is an IgG1-subtype. MAb chCE7 was produced in HEK293 cells and subsequently purified from cell culture supernatant as previously described by Grünberg et al. [34 (link)].

IGROV1 human ovarian cancer cells were kindly provided by Dr. Cristina Müller (Center for Radiopharmaceutical Sciences, Paul Scherrer Institute) and analysed by STR profiling (DSMZ, Braunschweig, Germany). IGROV1 cells were maintained in a humidified atmosphere containing 5% CO₂ in RPMI 1640 medium at 37°C. The medium was supplemented with 10% fetal calf serum (FCS), 2 mM glutamine, 100 units/ml penicillin, 100 μg/ml streptomycin and 0.25 μg/ml fungizone (BioConcept, Allschwil, Switzerland). mAb chCE7 is a IgG1-subtype chimeric monoclonal antibody (human κ light chain and human γ1 heavy chain). It was produced in HEK293 cells and purified from cell culture supernatant using a protein G-Sepharose column (GE Healthcare, Glattbrugg, Switzerland) as described by Grünberg et al. [32 (link)]. An unspecific isotype-matched IgG was used as a control for experiments.