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Anti pro caspase1 p10 p12 antibody

Manufactured by Abcam
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Sourced in United Kingdom

Anti-pro Caspase1+p10+p12 Antibody is a laboratory reagent used in research applications. It recognizes the pro-form of Caspase-1 as well as the p10 and p12 subunits of the active Caspase-1 enzyme.

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Lab products found in correlation

Nf kb p65, by Santa Cruz Biotechnology (2 mentions) Flag tag (flag), by Santa Cruz Biotechnology (2 mentions) Irgm antibody rodent specific, by Santa Cruz Biotechnology (2 mentions) Phsopho p38 mapk, by Santa Cruz Biotechnology (2 mentions) P nf kb p65, by Santa Cruz Biotechnology (2 mentions) Hrp conjugated secondary antibody, by Santa Cruz Biotechnology (2 mentions) C myc, by Santa Cruz Biotechnology (2 mentions) Nlrp3, by Adipogen (2 mentions) Caspase 1, by Adipogen (2 mentions) Anti gsdmd antibody, by Abcam (2 mentions) Anti caspase 3 antibody, by Cell Signaling Technology (1 mentions) Tmr red kit, by Roche (1 mentions) Cell lysis buffer 10, by Cell Signaling Technology (1 mentions) Lysotracker deep red, by Thermo Fisher Scientific (1 mentions) Anti f4 80 antibody, by BD (1 mentions) Lipofectamine 3000 transfection reagent, by Thermo Fisher Scientific (1 mentions) Anti caspase 11 antibody, by Abcam (1 mentions) Anti rip1, by Cell Signaling Technology (1 mentions) Anti gfp antibody, by Thermo Fisher Scientific (1 mentions) Anti cleaved caspase 3 antibody, by Cell Signaling Technology (1 mentions)

4 protocols using anti pro caspase1 p10 p12 antibody

1

Immunoblotting Antibody Validation Protocol

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Flag (1:1000), c-Myc (Santacruz-SC-40; 1:750 and sc-764; 1:1000), p62 (BD; 1:2000), Actin (Abcam; 1:5000), IRGM (Abcam; 1:500), HA (CST; 1:1000), IL-1β (CST; 1:1000), Caspase-1(Adipogen; 1:1000), NLRP3 (Adipogen; 1:1000), GFP (Abcam; 1:5000), ASC (Santacruz; 1:1000), ATG-5 (CST, 1:1000), ATG7 (CST, 1:1000), NLRC4 (CST; 1:1000) and AIM2 (CST; 1:1000), IRGM Antibody Rodent specific (CST; 1:1000), c-Myc (Santa Cruz; 1:750). Phsopho-p38 MAPK (CST, 1:1000), p-38α (Santa cruz, 1:1000), Anti-pro Caspase1+p10+p12 Antibody (Abcam; 1:1000), NF-kb p65 (Santa cruz; 1:1000), p- NF-kb p65 (Santa cruz; 1:1000).
HRP conjugated secondary antibodies were purchased from Santa cruz (1:2000) or Promega (1:5000) or Abcam (1:10000).
Mehto S., Jena K.K., Nath P., Chauhan S., Kolapalli S.P., Das S.K., Sahoo P.K., Jain A., Taylor G.A, & Chauhan S. (2019). The Crohn’s Disease Risk Factor IRGM Limits NLRP3 Inflammasome Activation by Impeding Its Assembly and by Mediating Its Selective Autophagy. Molecular Cell, 73(3), 429-445.e7.
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2

Immunoblotting Antibody Validation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Flag (1:1000), c-Myc (Santacruz-SC-40; 1:750 and sc-764; 1:1000), p62 (BD; 1:2000), Actin (Abcam; 1:5000), IRGM (Abcam; 1:500), HA (CST; 1:1000), IL-1β (CST; 1:1000), Caspase-1(Adipogen; 1:1000), NLRP3 (Adipogen; 1:1000), GFP (Abcam; 1:5000), ASC (Santacruz; 1:1000), ATG-5 (CST, 1:1000), ATG7 (CST, 1:1000), NLRC4 (CST; 1:1000) and AIM2 (CST; 1:1000), IRGM Antibody Rodent specific (CST; 1:1000), c-Myc (Santa Cruz; 1:750). Phsopho-p38 MAPK (CST, 1:1000), p-38α (Santa cruz, 1:1000), Anti-pro Caspase1+p10+p12 Antibody (Abcam; 1:1000), NF-kb p65 (Santa cruz; 1:1000), p- NF-kb p65 (Santa cruz; 1:1000).
HRP conjugated secondary antibodies were purchased from Santa cruz (1:2000) or Promega (1:5000) or Abcam (1:10000).
Mehto S., Jena K.K., Nath P., Chauhan S., Kolapalli S.P., Das S.K., Sahoo P.K., Jain A., Taylor G.A, & Chauhan S. (2019). The Crohn’s Disease Risk Factor IRGM Limits NLRP3 Inflammasome Activation by Impeding Its Assembly and by Mediating Its Selective Autophagy. Molecular Cell, 73(3), 429-445.e7.
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3

Immunoblotting Analysis of Inflammatory Pathways

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Anti-cleaved caspase-3 antibody, anti-caspase-3 antibody, anti-β-actin antibody, anti-RIP1 (receptor-interacting protein 1) antibody, anti-p-RIP1 (phospho-RIP1) antibody, cell lysis buffer (10×), and protease/phosphatase inhibitor cocktail were all from Cell Signaling Technology (Danvers, MA). Anti-caspase-11 antibody, anti-pro caspase-1+p10+p12 antibody, anti-GSDMD antibody, anti-GSDME antibody, and propidium iodide were from Abcam (Cambridge, UK). Anti-GFP antibody, Hoechst 33342, MitoTracker Deep Red FM, and LysoTracker Deep Red were from Invitrogen. Anti-F4/80 antibody was from BD Biosciences (Franklin Lakes, NJ). TMR red kit was from Roche (Basel, Switzerland). Lipofectamine 3000 Transfection Reagent was from Thermo Fisher Scientific.
Sun P., Zhong J., Liao H., Loughran P., Mulla J., Fu G., Tang D., Fan J., Billiar T.R., Gao W, & Scott M.J. (2021). Hepatocytes Are Resistant to Cell Death From Canonical and Non-Canonical Inflammasome-Activated Pyroptosis. Cellular and Molecular Gastroenterology and Hepatology, 13(3), 739-757.
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4

GSDMD, Caspase-1, and IL-1β Detection

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Anti-GSDMD antibody, anti-pro-caspase-1 + p10 + p12 antibody, and anti-IL-1β antibody for western blotting were all purchased from Abcam (Cambridge, UK). Anti-NLRP3 antibody was purchased from Wanleibio (Shenyang, China). Anti-GSDMD antibody for immunofluorescence was purchased from Bioss (Beijing, China). MCC950 (NLRP3 inhibitor) was purchased from MedChemExpress (Monmouth Junction, NJ, USA). rTFPI was purchased from R&D (Minneapolis, MN, USA).
Dai Y., Xu R., Yang K., Jiang T., Wei Y., Liu Y., Chen W., Fu Y, & Zhao Y. (2024). Effect of tissue factor pathway inhibitor on the pyroptosis of vascular smooth muscle cells induced by angiotensin II. Cardiovascular Diagnosis and Therapy, 14(1), 72-83.
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