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4 protocols using anti pro caspase1 p10 p12 antibody

1

Immunoblotting Antibody Validation Protocol

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Flag (1:1000), c-Myc (Santacruz-SC-40; 1:750 and sc-764; 1:1000), p62 (BD; 1:2000), Actin (Abcam; 1:5000), IRGM (Abcam; 1:500), HA (CST; 1:1000), IL-1β (CST; 1:1000), Caspase-1(Adipogen; 1:1000), NLRP3 (Adipogen; 1:1000), GFP (Abcam; 1:5000), ASC (Santacruz; 1:1000), ATG-5 (CST, 1:1000), ATG7 (CST, 1:1000), NLRC4 (CST; 1:1000) and AIM2 (CST; 1:1000), IRGM Antibody Rodent specific (CST; 1:1000), c-Myc (Santa Cruz; 1:750). Phsopho-p38 MAPK (CST, 1:1000), p-38α (Santa cruz, 1:1000), Anti-pro Caspase1+p10+p12 Antibody (Abcam; 1:1000), NF-kb p65 (Santa cruz; 1:1000), p- NF-kb p65 (Santa cruz; 1:1000).
HRP conjugated secondary antibodies were purchased from Santa cruz (1:2000) or Promega (1:5000) or Abcam (1:10000).
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2

Immunoblotting Antibody Validation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Flag (1:1000), c-Myc (Santacruz-SC-40; 1:750 and sc-764; 1:1000), p62 (BD; 1:2000), Actin (Abcam; 1:5000), IRGM (Abcam; 1:500), HA (CST; 1:1000), IL-1β (CST; 1:1000), Caspase-1(Adipogen; 1:1000), NLRP3 (Adipogen; 1:1000), GFP (Abcam; 1:5000), ASC (Santacruz; 1:1000), ATG-5 (CST, 1:1000), ATG7 (CST, 1:1000), NLRC4 (CST; 1:1000) and AIM2 (CST; 1:1000), IRGM Antibody Rodent specific (CST; 1:1000), c-Myc (Santa Cruz; 1:750). Phsopho-p38 MAPK (CST, 1:1000), p-38α (Santa cruz, 1:1000), Anti-pro Caspase1+p10+p12 Antibody (Abcam; 1:1000), NF-kb p65 (Santa cruz; 1:1000), p- NF-kb p65 (Santa cruz; 1:1000).
HRP conjugated secondary antibodies were purchased from Santa cruz (1:2000) or Promega (1:5000) or Abcam (1:10000).
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3

Immunoblotting Analysis of Inflammatory Pathways

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Anti-cleaved caspase-3 antibody, anti-caspase-3 antibody, anti-β-actin antibody, anti-RIP1 (receptor-interacting protein 1) antibody, anti-p-RIP1 (phospho-RIP1) antibody, cell lysis buffer (10×), and protease/phosphatase inhibitor cocktail were all from Cell Signaling Technology (Danvers, MA). Anti-caspase-11 antibody, anti-pro caspase-1+p10+p12 antibody, anti-GSDMD antibody, anti-GSDME antibody, and propidium iodide were from Abcam (Cambridge, UK). Anti-GFP antibody, Hoechst 33342, MitoTracker Deep Red FM, and LysoTracker Deep Red were from Invitrogen. Anti-F4/80 antibody was from BD Biosciences (Franklin Lakes, NJ). TMR red kit was from Roche (Basel, Switzerland). Lipofectamine 3000 Transfection Reagent was from Thermo Fisher Scientific.
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4

GSDMD, Caspase-1, and IL-1β Detection

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Anti-GSDMD antibody, anti-pro-caspase-1 + p10 + p12 antibody, and anti-IL-1β antibody for western blotting were all purchased from Abcam (Cambridge, UK). Anti-NLRP3 antibody was purchased from Wanleibio (Shenyang, China). Anti-GSDMD antibody for immunofluorescence was purchased from Bioss (Beijing, China). MCC950 (NLRP3 inhibitor) was purchased from MedChemExpress (Monmouth Junction, NJ, USA). rTFPI was purchased from R&D (Minneapolis, MN, USA).
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